Rabbit anti rig 1

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-RIG-I is a primary antibody that recognizes the RIG-I (Retinoic Acid-Inducible Gene I) protein. RIG-I is a pattern recognition receptor that plays a crucial role in the innate immune response by detecting viral RNA and initiating a signaling cascade that leads to the production of type I interferons and other antiviral cytokines.

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Lab products found in correlation

Close spelling variants of this product

RIG-I (37 citations) Anti-RIG-I (28 citations) Rabbit anti-RIG-I (D14G6) (4 citations)

5 protocols using rabbit anti rig 1

Western Blotting Analysis of Cell Signaling

Cited 1 time

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Western blotting was performed as described earlier [45 (link)] using rabbit anti-RIG-I, rabbit anti-phospho-cdc2(Tyr15), rabbit anti-phospho-Chk2(Thr68) (Cell Signaling Technology Inc., Boston, MA, USA), and rabbit anti-DUSP6 (Abcam) monoclonal antibodies. In addition, rabbit anti-cyclinB1 and rabbit anti-CDK1(cdc2), and rabbit anti-Chk2 (Proteintech Group Inc., Wuhan, China) polyclonal antibodies were also used. All primary antibodies were diluted at 1:1000. The same membrane was stripped and reprobed with mouse anti-GAPDH, anti-β-actin, and anti-α-tubulin monoclonal antibodies (Proteintech Group Inc.) as internal controls.

Li L., Lv L., Xu J.C., He Q., Chang N., Cui Y.Y., Tao Z.C., Zhu T, & Qian L.T. (2023). RIG-I Promotes Tumorigenesis and Confers Radioresistance of Esophageal Squamous Cell Carcinoma by Regulating DUSP6. International Journal of Molecular Sciences, 24(6), 5586.

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Primary Antibodies Used in Research

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The following primary antibodies were used: rabbit anti-CypA (Enzo; BML-SA296-0100), rabbit anti-CypB (Abcam; ab16045), rabbit anti-PKR (Abcam; ab32035), rabbit anti- RIG-I (Cell Signalling; 3743), mouse-anti-β-Actin (Sigma Aldrich; A1978), sheep anti-GST (in-house) and sheep-anti-NS5A (in-house) [22 (link)]. Secondary IRDye 680 and 800 labelled antibodies were obtained from Li-COR.

Chen S, & Harris M. (2023). Mutational analysis reveals a novel role for hepatitis C virus NS5A domain I in cyclophilin-dependent genome replication. The Journal of general virology, 104(9), 10.1099/jgv.0.001886.

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Immunoblotting Antibody Validation for IFN-γ Signaling

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The antibodies were listed above for flow cytometry at in vitro coculture assay. For immunoblotting, the following antibodies were used: mouse anti–MHC-I (Santa Cruz Biotechnology, sc-55582), rabbit anti-pSTAT1 (Cell Signaling Technology, 7649), rabbit anti-STAT1 (CST, 14994), rabbit anti-IFNGR1 (Millipore, MABF753), mouse anti–β-actin (Sigma-Aldrich, A5441), rabbit anti–RIG-I (Cell Signaling Technology, 3743), rabbit anti–MDA-5 (Cell Signaling Technology, 5321), rabbit anti-MAVS (Cell Signaling Technology, 3993), rabbit anti-pIRF3 (Cell Signaling Technology, 29047), rabbit anti-IRF3 (Cell Signaling Technology, 4302), rabbit anti-p-p65 (Cell Signaling Technology, 3033), rabbit anti-p65 (Cell Signaling Technology, 8242), rabbit anti-pSTING (Cell Signaling Technology, 72971), and rabbit anti-STING (Cell Signaling Technology, 13647). rabbit anti-p65 (Cell Signaling Technology, 8242) was used for RIP and ChIP, and normal rabbit immunoglobulin G (IgG; Cell Signaling Technology, 2729) was served as a negative control.

Wang Y., Zhao Y., Guo W., Yadav G.S., Bhaskarla C., Wang Z., Wang X., Li S., Wang Y., Chen Y., Pattarayan D., Xie W., Li S., Lu B., Kammula U.S., Zhang M, & Yang D. (2022). Genome-wide gain-of-function screening characterized lncRNA regulators for tumor immune response. Science Advances, 8(49), eadd0005.

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Western Blotting of Innate Immune Sensors

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Cells (1–1.2 × 10⁶) were lysed in 50 mM Tris-HCl pH 8, 150 mM NaCl, 1% Triton X-100, and 1 mM EDTA with the addition of protease inhibitors (cOmplete, Roche). Protein samples were separated at 100 V by 10% SDS-PAGE electrophoresis and blotted on a nitrocellulose membrane. Membranes were incubated with specific antibodies: mouse anti-α-tubulin (1:8000, Sigma-Aldrich), rabbit anti-MDA5 (1:800, Cell Signaling), rabbit anti-RIGI (1:800, Cell Signaling), rabbit anti-fibrillarin (1:10,000, Abcam). HRP-conjugated anti-rabbit (1:80,000) and anti-mouse (1:5000) antibodies were purchased from Sigma-Aldrich. Proteins were revealed with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). All immunoblots blots were performed in triplicate and the quantification of the protein bands from all three experiments is shown in each figure.

Wiatrek D.M., Candela M.E., Sedmík J., Oppelt J., Keegan L.P, & O'Connell M.A. (2019). Activation of innate immunity by mitochondrial dsRNA in mouse cells lacking p53 protein. RNA, 25(6), 713-726.

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Immunoblotting Techniques for Protein Analysis

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SDS/PAGE and Western blotting were performed as previously described (63 (link)). The following antibodies were purchased from Thermo Fisher Scientific: Mouse anti-Puromycin (MABE343MI), Rabbit anti-Actin (PIPA1183), Mouse anti-SYNCRIP (MAB11004MI). The following antibodies were purchased from Abcam (Toronto, ON, Canada): Rabbit anti-PABP (ab21060), Rabbit anti-CSDE1 (ab201688), Rabbit anti-TRIM25 (ab167154), Rabbit anti-PABPC4 (ab220832, Abcam), Rabbit anti-PABPN1 (ab75855), Mouse anti-HRNPNK (ab39975). The following antibodies were purchased from Proteintech (Rosemont, IL, USA): Rabbit anti-SRP9 (11195-1-AP), Rabbit anti-SRP14 (11528-1-AP), Rabbit anti-STRAP (18277-1-AP). The following additional antibodies were used: Mouse anti-Tubulin (T6074, Sigma-Aldrich), Rabbit anti-RIG-I (3743S, Cell Signaling Technologies, Danvers, MA, USA), Rabbit anti-EIF3K (CSB-PA866204ESR1HU, CusaBio), and Mouse anti-DHX36 (Clone 12F33, made in-house).

Booy E.P., Gussakovsky D., Choi T, & McKenna S.A. (2020). The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells. The Journal of Biological Chemistry, 296, 100036.

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