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2 protocols using fluorescent conjugated anti human antibodies

1

Mesenchymal Stem Cell Differentiation

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Dulbecco’s modified eagle’s medium (DMEM), Oil red O, alizarin red, dexamethasone, iso butyl methyl xanthine, indomethacin, insulin, β- glycerophosphate, ascorbic acid and basic fibroblast growth factor were purchased from Sigma Aldrich (Steinheim, Germany). Tissue culture plastic plates and flasks were from Eppendorf (Germany). Fluorescent conjugated anti-human antibodies were from BD biosciences (Germany). Fetal bovine serum (FBS) and real-time PCR reagents were purchased from Thermo Fisher scientific (USA).
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2

Adipogenic and Chondrogenic Differentiation

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Dulbecco’s modified eagle’s medium (DMEM), fibronectin, leukocyte alkaline phosphatase kit, Oil red O, Safranin O, dexamethasone, iso butyl methyl xanthine, indomethacin, insulin, β- glycerophosphate and ascorbic acid were purchased from Sigma Aldrich (Steinheim, Germany). Tissue culture plastic plates and flasks were from BD biosciences (Heidelberg, Germany). Fluorescent conjugated anti-human antibodies were from BD biosciences. Anti-Oct4 antibody was from Santa Cruz. Fetal bovine serum (FBS), recombinant human BDNF, chondrogenic differentiation media, neurobasal media, neuronal supplements and Tetramethylrhodamine, ethyl ester (TMRE) were purchased from Thermofisher scientific (Paisley, UK).
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