Chod iodide test kit

The study was approved by the appropriate institutional review board (ethics committee of the Medical University of Graz; 27–320 ex 14/15) and written informed consent was obtained. Human LDL (1.020 to 1.063 g/mL) was obtained from the plasma of normolipemic (Lp(a) < 5 mg/dL), fasting (12 to 14 h) male donors (a total of 7 healthy volunteers aged between 29 and 44 years) by potassium bromide sequential ultracentrifugation [12 (link)]. Pefabloc (50 μM, Sigma-Aldrich, Vienna, Austria), butylated hydroxytoluene (20 μM, Sigma-Aldrich), and EDTA (1 g/L, Merck, Darmstadt, Germany) were present during all steps of lipoprotein preparation to prevent lipid peroxidation and apoB cleavage by contaminating bacteria or proteinases. The samples were sterile-filtered and stored at 4°C in the dark until use. The protein content of LDL was measured using the Lowry method [13 (link)]. Total cholesterol of the isolated LDL was determined enzymatically with the CHOD-iodide test kit (Boehringer-Mannheim, Germany).

The appropriate institutional review board (ethics committee of the Medical University of Graz; 27-320 ex 14/15) approved this study. Written informed consent was obtained from all participants. Human LDL (1.020 to 1.063 g/mL) was obtained from the plasma of four normolipemic (Lp(a) < 5 mg/dL), fasting (12 to 14 h) male young individuals by sequential ultracentrifugation with potassium bromide [27 (link),28 (link)]. Pefabloc (50 µM, Sigma Aldrich, Vienna, Austria), EDTA (1 g/L, Merck, Darmstadt, Germany), and butylated hydroxytoluene (BHT, 20 µM, Sigma, St. Louis, MO, USA) were present during all steps of lipoprotein preparation in order to prevent lipid peroxidation and apolipoprotein B (apoB) cleavage by proteinases and possible contaminating bacteria. The samples were sterile filtered and stored at 4 °C in the dark until use. The Lowry method was applied to measure the protein content of LDL [29 (link)]. Total cholesterol of the isolated LDL was determined enzymatically using the CHOD-iodide test kit (Boehringer-Mannheim, Mannheim, Germany).