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Inh h151

Manufactured by InvivoGen
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The Inh-h151 is a lab equipment product from InvivoGen. It is a cell inhibitor that targets a specific molecular pathway. The core function of the Inh-h151 is to inhibit the activity of the targeted molecule.

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Lab products found in correlation

Carbonyl cyanide 4, by Merck Group (2 mentions) Ru360, by Merck Group (2 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) C576065, by Cell Biologics (1 mentions) M1168, by Cell Biologics (1 mentions) Cc 3202, by Lonza (1 mentions) S6575, by Selleck Chemicals (1 mentions) S1537, by Selleck Chemicals (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Vac nacga23, by InvivoGen (1 mentions) Bms6002, by Thermo Fisher Scientific (1 mentions) Synergy h1 hybrid reader, by Agilent Technologies (1 mentions) Inh ru521, by InvivoGen (1 mentions)

6 protocols using inh h151

1

Endothelial Cell Senescence Assays

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hRECs (PB-CH-160-8511, Pelo Bioscience) were maintained in EGM-2 MV medium (CC-3202, Lonza) under treatment conditions for 2 weeks. In addition to the 2 treatment conditions with 1000 U IFN-β (8499-IF-010, R&D Systems) and 33 μg/mL cGAMP (tlrl-nacga23m, InvivoGen), a control arm was performed. Meanwhile, the cells underwent 3 passages (p7–p9) and the medium was changed every other or every third day. After the last passaging step, cells were split into a 96-well plate for the readout assay.
Primary mRECs (C576065, Cell Biologics) were cultured in Complete Mouse Endothelial Cell Medium (M1168, Cell Biologics) at 37°C in an incubator containing 5% CO2. The medium was changed every other day until the cells were confluent for use. For the flow cytometry cell senescence assay and the ELISA experiment, mRECs were seeded in a 6-well plate at a concentration of 0.2 million cells per well. For the confocal imaging cell senescence assay, mRECs were seeded on a cell culture cover glass (NC0620709, Thermo Fisher Scientific) and laid on the bottom of a 24-well plate (3527, Corning) at 0.1 million cells per well. After 24 hours of incubation, mRECs were treated with 0.1% DMSO (control), cGAMP (20 μg/mL), STING inhibitor (2 μg/mL; inh-h151, Invivogen), or IFN-β (1000 U/mL; 12400-1, R&D Systems) for an additional 1, 2, or 4 days, respectively.
Liu H., Ghosh S., Vaidya T., Bammidi S., Huang C., Shang P., Nair A.P., Chowdhury O., Stepicheva N.A., Strizhakova A., Hose S., Mitrousis N., Gadde S.G., MB T., Strassburger P., Widmer G., Lad E.M., Fort P.E., Sahel J.A., Zigler JS J.r., Sethu S., Westenskow P.D., Proia A.D., Sodhi A., Ghosh A., Feenstra D, & Sinha D. (2023). Activated cGAS/STING signaling elicits endothelial cell senescence in early diabetic retinopathy. JCI Insight, 8(12), e168945.
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2

Calcification Modulation in RH30 Cells

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RH30 cells are cultured in 24-well tissue culture plate at the concentration of 200,000 cells per well and allowed to adhere overnight (o/n) before o/n treatment with complete growth medium supplemented with following concentrations of CaCl2 and Na3PO4: Very Low CaP: 0.7575 mM CaCl2 and 5.63 mM Na3PO4, Low CaP: 1.095 mM CaCl2 and 6.13 mM Na3PO4; Moderate CaP: 1.77 mM CaCl2 and 6.63 mM Na3PO4 for different time points. Different reagents used in calcification experiments include Ru360 (557,440, Sigma-Aldrich), Antimycin A (A8674, Sigma-Aldrich), Carbonyl cyanide 4-(trifluoromethoxy) phenyl-hydrazone Ready Made Solution, FCCP (SML2959, Sigma-Aldrich), Universal Type I IFN Protein (Recombinant Human IFN-alpha A/D [BglII]), 11,200–1, R&D systems), H-151 (inh-h151, InvivoGen), and VBIT-4 (S3544, Selleckchem).
Duvvuri B., Pachman L.M., Hermanson P., Wang T., Moore R., Wang D.D., Long A., Morgan G.A., Doty S., Tian R., Sancak Y, & Lood C. (2023). Role of mitochondria in the myopathy of juvenile dermatomyositis and implications for skeletal muscle calcinosis. Journal of autoimmunity, 138, 103061.
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3

Calcification Modulation in RH30 Cells

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RH30 cells are cultured in 24-well tissue culture plate at the concentration of 200,000 cells per well and allowed to adhere overnight (o/n) before o/n treatment with complete growth medium supplemented with following concentrations of CaCl2 and Na3PO4: Very Low CaP: 0.7575 mM CaCl2 and 5.63 mM Na3PO4, Low CaP: 1.095 mM CaCl2 and 6.13 mM Na3PO4; Moderate CaP: 1.77 mM CaCl2 and 6.63 mM Na3PO4 for different time points. Different reagents used in calcification experiments include Ru360 (557,440, Sigma-Aldrich), Antimycin A (A8674, Sigma-Aldrich), Carbonyl cyanide 4-(trifluoromethoxy) phenyl-hydrazone Ready Made Solution, FCCP (SML2959, Sigma-Aldrich), Universal Type I IFN Protein (Recombinant Human IFN-alpha A/D [BglII]), 11,200–1, R&D systems), H-151 (inh-h151, InvivoGen), and VBIT-4 (S3544, Selleckchem).
Duvvuri B., Pachman L.M., Hermanson P., Wang T., Moore R., Wang D.D., Long A., Morgan G.A., Doty S., Tian R., Sancak Y, & Lood C. (2023). Role of mitochondria in the myopathy of juvenile dermatomyositis and implications for skeletal muscle calcinosis. Journal of autoimmunity, 138, 103061.
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4

Cytokine-induced Macrophage Activation

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THP-1 cells were differentiated with 100 ng/ml PMA (P1585,Sigma) for 24 h then treated with 100 ng/ml IFN-γ (300-02, PeproTect), or 50 ng/ml IL-6 (200-06, PeproTect), otherwise 20 ng/ml IL-4 (200-04, PeproTect) for another 24 h. RAW264.7s were incubated with 100 ng/ml IFN-γ (315-05, PeproTect), or 50 ng/ml IL-6 (216-16, PeproTect), or 20 ng/ml IL-4 (214-14, PeproTect), or 1 µg/ml LPS (L4524, Sigma)for 24 h. RAW264.7s and THP-1s were incubated with medium containing 1% (v/v) FBS for 24 h, after stimulation as indicated. Cell supernatants were harvested as conditional medium (CM) by centrifugation at 1500 g for 5 min to remove all the debris. If not otherwise indicated, small molecule compounds, inhibitors, agonists were added to the cells 2 h before stimulation using the following concentrations: 2 µm PP (CAS No.76296-75-8, Apexbio, Houston) for RAW264.7 and 1 µm PP for THP-1, 2 µm C-176 (S6575, Selleck) and 50 µg/ml DMXAA (S1537, Selleck) for RAW264.7, 1 µm H-151 for THP-1 (inh-h151, invivoGen).
Yu J., Deng H, & Xu Z. (2020). Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer. Scientific Reports, 10, 21360.
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5

Measuring Type I IFN Levels in Mouse Retina

Cited 1 time
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Mouse IFN-α (42115-1, pbl Assay Science) and IFN-β (42410, pbl Assay Science) ELISA kits were used to measure the level of IFN-β in mouse retinal and primary mREC culture medium. Mice were euthanized by CO2 asphyxiation. The retina was freshly isolated and sonicated in a sample buffer provided in the ELISA kit with 1% phosphatase and 1% protease inhibitors. The mREC culture medium was collected 4 days after cGAMP (20 μg/mL; vac-nacga23, Invivogen) and STING inhibitor (2 μg/mL; inh-h151, Invivogen) treatment. Following the kit instructions, the absorbance of each sample was measured by a microplate reader (BioTek Synergy H1 Hybrid reader) at 450 nm. Lastly, the IFN titer of the samples was determined by plotting the optical densities using a 4-parameter fit for the standard curve in GraphPad Prism 9 (version 9.3.1). The retinal explants from WT, STING-KO, and STINGGT mice were cultured in presence or absence of LG or HG as explained above and in a previous report (55 (link)). The spent media from the cultures were analyzed by ELISA for HMGB1 (Novus Biologicals, NBP2-62767) and IL-1β (Invitrogen, BMS6002) using commercially available kits and following the manufacturer’s guidelines.
Liu H., Ghosh S., Vaidya T., Bammidi S., Huang C., Shang P., Nair A.P., Chowdhury O., Stepicheva N.A., Strizhakova A., Hose S., Mitrousis N., Gadde S.G., MB T., Strassburger P., Widmer G., Lad E.M., Fort P.E., Sahel J.A., Zigler JS J.r., Sethu S., Westenskow P.D., Proia A.D., Sodhi A., Ghosh A., Feenstra D, & Sinha D. (2023). Activated cGAS/STING signaling elicits endothelial cell senescence in early diabetic retinopathy. JCI Insight, 8(12), e168945.
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6

Evaluating Inhibitor Effects on Cell Transfection

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Cells were seeded onto sterile 96-well plates as described above. The cells were cultured overnight in media containing various concentrations of RU 521 (0.2 µg/mL, 1 µg/mL, 2 µg/mL, and 10 µg/mL, #inh-ru521, InvivoGen) or H-151 (0.5 µM, 1 µM, 5 µM, and 10 µM, #inh-h 151, InvivoGen) and then incubated with DNA or DNA–NP complexes. Experiments were carried out in triplicate. DNA treatments had a DNA concentration of 1 µg/mL. Cell treatments were carried out in Opti-MEM containing respective concentrations of RU 521 and H-151. After a 24-h incubation, the cell media was harvested and centrifuged at 3,000 rcf for 15 min to remove any cell debris. The supernatant was collected and stored at −20 °C until the ELISA was conducted.
Anees F., Montoya D.A., Pisetsky D.S, & Payne C.K. (2024). DNA corona on nanoparticles leads to an enhanced immunostimulatory effect with implications for autoimmune diseases. Proceedings of the National Academy of Sciences of the United States of America, 121(11), e2319634121.
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