Mouse fc block clone 2.4g2

Manufactured by BD

Mouse Fc block (clone 2.4G2) is a laboratory product designed to block Fc receptors on mouse cells. It binds to the Fc portion of mouse immunoglobulins, preventing their interaction with Fc receptors.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Perm wash buffer, by BD (1 mentions) Golgistop, by BD (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Cell staining buffer, by BioLegend (1 mentions) Anti cd28, by BD (1 mentions) Anti cd3e, by BioLegend (1 mentions) Ifn γ apc clone xmg1.2, by BioLegend (1 mentions) Fixation permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Il 17 pe clone tc11 18h10, by BioLegend (1 mentions) Cd4 apc cy7 clone rm4 5, by BioLegend (1 mentions) Cd8a percp cy5 5 clone 53 6, by BD (1 mentions) Cd4 fitc, by Thermo Fisher Scientific (1 mentions) Cd3 pe, by Thermo Fisher Scientific (1 mentions) Cd11c apc, by Thermo Fisher Scientific (1 mentions) Cd8a efluor 450, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Fc block (765 citations) Mouse BD Fc Block (226 citations) Fc block (2.4G2, (41 citations) Fc Block (clone 2.4G2; (27 citations) Clone 2.4G2 (24 citations) Mouse Fc block (2.4G2), (4 citations) Anti-mouse CD16/CD32 Fc Block (clone 2.4G2, (2 citations) Anti-mouse CD16/CD32 (mouse BD Fc block) mAb clone 2.4G2 (2 citations)

2 protocols using mouse fc block clone 2.4g2

Cytokine Profiling of CNS-infiltrating T cells

Cited 1 time

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To assess cytokine production on T cells infiltrating the CNS, immunized mice were sacrificed at peak disease and mononuclear cells from brain tissues were isolated as previously described (Garcia et al., 2013 (link)). T cells were stimulated for 4 h at 37°C and 5% CO₂ under the following conditions: cRMPI [RPMI 1640, Gibco; 10% Fetal bovine serum, Atlanta Biologicals; 1% Pen-Strep (Gibco), anti-CD3e (BioLegend), anti-CD28 (BD Pharmingen) and GolgiStop (BD Biosciences)]. For intracellular cytokine staining, the following reagents were used: Mouse Fc block (clone 2.4G2, BD Pharmingen); fixation/permeabilization buffer (Invitrogen); perm/wash buffer (BD Biosciences); cell staining buffer (BioLegend) and the following antibody cocktail: TCR-b V450 (clone H57-597, BD Horizon), CD4-APC-Cy7 (clone RM4-5, BioLegend), CD8a-PerCP/Cy5.5 (clone 53-6.7, BD Pharmingen), IL-17-PE (clone TC11-18H10.1, BioLegend) and IFN-γ-APC (clone XMG1.2, BioLegend) Samples were acquired using an ImageStreamX-Imaging Flow Cytometer-ISX-MKII (EMD Millipore) at the Cell Analysis Core, UTSA. Data analysis was performed using IDEAS software version 6.2.

Cardona S.M., Kim S.V., Church K.A., Torres V.O., Cleary I.A., Mendiola A.S., Saville S.P., Watowich S.S., Parker-Thornburg J., Soto-Ospina A., Araque P., Ransohoff R.M, & Cardona A.E. (2018). Role of the Fractalkine Receptor in CNS Autoimmune Inflammation: New Approach Utilizing a Mouse Model Expressing the Human CX3CR1I249/M280 Variant. Frontiers in Cellular Neuroscience, 12, 365.

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Multicolor Flow Cytometry Panel

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Cells were blocked with CD16/CD32 (Mouse Fc Block, Clone 2.4G2; BD Pharmingen, Franklin Lakes, NJ) for 15 minutes on ice, then incubated with antibodies to the proteins of interest (45 minutes, on ice). Antibodies were from eBiosciences (San Diego, CA): CD3‐PE (phycoerythrin) (clone 145‐2C11); CD4‐FITC (Clone GK1.5); CD8a‐eFluor450 (Clone 53‐6.7); CD11b‐allophycocyanin (APC)‐eFluor780 (Clone M1/70); CD11c‐APC (Clone N418); CD45R(B220)‐PE‐cyanin 7 (Clone RA3‐6B2); and major histocompatibility complex (MHC) class II (I‐A/I‐E)‐APC (Clone M5/114.15.2). Staining for FcγRI expression was done using CD64‐PE (Clone 290322) from R&D Systems (Minneapolis, MN). Unstained cells were used to establish flow cytometer settings. Single‐color positive controls were used for compensation. Flow cytometric data were acquired on a FacsCalibur (Becton and Dickenson, Franklin Lakes, NJ) using FlowJo. The data were analyzed with FlowJo Software (Tree Star, Ashland, OR).

Harmon E.Y., Fronhofer V., Keller R.S., Feustel P.J., Zhu X., Xu H., Avram D., Jones D.M., Nagarajan S, & Lennartz M.R. (2014). Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(6), e001232.

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