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Malvern zetasize nano zs90

Manufactured by Malvern Panalytical
Sourced in United Kingdom

The Malvern Zetasize Nano-zs90 is a dynamic light scattering (DLS) instrument designed for the measurement of particle size and zeta potential in nanomaterials and colloidal systems. It determines the size of particles or molecules in suspension by measuring their Brownian motion and relating this to their size using the Stokes-Einstein equation. The instrument can measure particle sizes ranging from 0.3 nanometers to 10 micrometers.

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4 protocols using malvern zetasize nano zs90

1

Aniseed Essential Oil Nano-Emulsion Preparation

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The NE of aniseed EO was prepared following the method of Hamouda et al. [74 (link)] with slight modifications [29 (link),75 (link)]. Briefly, coarse emulsion was prepared by mixing aniseed EO (14% v/v), ethanol (3% v/v), and biosurfactant non-ionic Tween 80 (3% v/v), representing 20% (v/v) of the total emulsion [29 (link)]. Then, the coarse emulsion was mixed and kept for 1 h at 86 °C. It was subsequently mixed with water (80%), kept for 3 min at room temperature (25 ± 3 °C) and finally centrifuged at 10,000× g. The aniseed NE was stored in dark bottles at ambient temperature until further analysis.
The aniseed NE was characterized by assessing the droplet size distribution (analysis by volume), which was determined by the dynamic laser light-scattering method (DLS). The zeta potential and polydispersity index PDI were investigated by photon correlation spectroscopy using a ZetaPlus tool (Malvern Zetasize Nano-zs90, Malvern Instruments Ltd., Enigma Business Park, Grovewood Road, Malvern, Worcestershire WR14 1XZ, UK) [29 (link)].
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2

Characterization of ZnO/Alg-NCMs Nanoparticles

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The characterization of ZnO/Alg-NCMs was performed by Fourier transform infrared spectroscopy (FTIR) using a (JASCO Europe S.r.l., Cremella, Italy), FT-IR 5300 spectrophotometer. Morphology and particle size of the nanoparticles were characterized via scanning electron microscopy JEOL JSM-6510/v, Tokyo, Japan (SEM), and transmission electron microscopy (JEOL JSM-6510/v, Tokyo, Japan), (TEM). The chemical contents of the nanoparticles were scanned by energy dispersive X-ray spectroscopy (EDS) using a Zeta potential analyser (Malvern Zeta size Nano-Zs90) Malvern United States.
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3

Characterization of GMP-BSA Particles

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The shape and surface morphology of the GMP-BSA were observed using confocal microscopy and a JSM-7500F field-emission scanning electron microscope (SEM; JEOL, Tokyo, Japan). For the confocal laser-scanning microscopy (CLSM) observation, the particles were first fluorescently labeled with rhodamine B or FITC on the glucan particle shell or BSA as described earlier, and then particle morphology and protein distribution were observed by confocal microscopy (Nikon Eclipse TE300; MRC-1024; BioRad, Hercules, CA, USA). The particles were also characterized for size with a Malvern Zetasize Nano ZS90 (Malvern Instruments, Malvern, UK).
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4

Characterization of Fe3O4@ZIF-8 Nanocomposite

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The morphology and structure of Fe3O4@ZIF-8 and DOX- Fe3O4@ZIF-8 were observed by transmission electron microscopy (TEM, OLYMPUS, Japan) and scanning electron microscopy (SEM, JSM-6309LV, Japan), and particle size distribution was analyzed in triplicate by Malvern Zeta size NanoZS90 at 25°C, scattering Angle 90°(British Malvern Instrument Co. Ltd, UK). The composition was determined by X-ray diffraction of the samples (PXRD, Malvern Nano ZS, UK), and the samples were characterized by Fourier infrared spectrometer 9 from Bruker (FT-IR), FTIR analyses were conducted in terms of DFZ at the speed of 2 mm/s by and in the range of 500–4000/cm (VERTEX 70, Broker, Germany). The loading efficiency and release rate were measured by UV-vis spectrophotometer (UH4150, Japan).
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