Aleuria aurantia lectin‐biotin and wheat germ hemagglutinin (WGA) were purchased from J‐OIL MILLS (Tokyo, Japan). Sambucus nigra lectin (SNA‐1) and its horseradish peroxidase (HRP) and biotin derivatives were obtained from EY Laboratories, Inc. (San Mateo, CA). Mouse monoclonal antibodies against sialyl‐Lewisa (CA19‐9, NS19‐9) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Anti‐rabbit IgG‐HRP conjugate and anti‐mouse IgG‐HRP conjugate were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). BlotGlyco beads were obtained from Sumitomo Bakelite Co., Ltd. (Tokyo, Japan).
Blotglyco beads
Manufactured by Sumitomo Bakelite
Sourced in Japan
BlotGlyco beads are a type of laboratory equipment used for the analysis of glycans. They are composed of agarose-based beads that can be used for the enrichment and purification of glycoproteins and glycopeptides from complex biological samples. BlotGlyco beads are designed to provide a simple and efficient method for the isolation and analysis of glycosylated biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Autoflex speed, by Bruker (2 mentions)
Anti rabbit igg hrp conjugate, by Jackson ImmunoResearch (1 mentions)
Anti mouse igg hrp conjugate, by Jackson ImmunoResearch (1 mentions)
Human serum, by Merck Group (1 mentions)
Peptide n glycosidase f, by Roche (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride tcep, by Merck Group (1 mentions)
Pngase f, by Roche (1 mentions)
4 protocols using blotglyco beads
1
Glycan Analysis Using Lectins and Antibodies
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Aleuria aurantia lectin‐biotin and wheat germ hemagglutinin (WGA) were purchased from J‐OIL MILLS (Tokyo, Japan). Sambucus nigra lectin (SNA‐1) and its horseradish peroxidase (HRP) and biotin derivatives were obtained from EY Laboratories, Inc. (San Mateo, CA). Mouse monoclonal antibodies against sialyl‐Lewisa (CA19‐9, NS19‐9) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Anti‐rabbit IgG‐HRP conjugate and anti‐mouse IgG‐HRP conjugate were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). BlotGlyco beads were obtained from Sumitomo Bakelite Co., Ltd. (Tokyo, Japan).
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Aleuria aurantia lectin‐biotin and wheat germ hemagglutinin (WGA) were purchased from J‐OIL MILLS (Tokyo, Japan). Sambucus nigra lectin (SNA‐1) and its horseradish peroxidase (HRP) and biotin derivatives were obtained from EY Laboratories, Inc. (San Mateo, CA). Mouse monoclonal antibodies against sialyl‐Lewisa (CA19‐9, NS19‐9) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Anti‐rabbit IgG‐HRP conjugate and anti‐mouse IgG‐HRP conjugate were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). BlotGlyco beads were obtained from Sumitomo Bakelite Co., Ltd. (Tokyo, Japan).
2
Serum Glycan Analysis using TCEP
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Human serum and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Disialyloctasaccharide (A2GN1) and methylamine (MeNH2) were purchased from FUJI FILM Wako Pure Chemical Corporation (Osaka, Japan). C 57BL/6NCr mice were maintained and six-month-old mice were used for glycemic analysis (n = 6). All experiments using laboratory animals were approved by the animal experiment committee of the Tokyo Metropolitan Institute of Gerontology and carried out according to its guidelines (Permit Number: 17010, 18,006). Peptide-N-Glycosidase F (PNGase F) was purchased from Roche (Mannheim, Germany). O-Benzylhydroxylamine was purchased from Tokyo Chemical Industry (Tokyo, Japan). BlotGlyco beads were purchased from Sumitomo Bakelite Co., Ltd. (Tokyo, Japan). MultiScreen Solvinert 0.45 μm low-binding hydrophilic polytetrafluoroethylene plates were purchased from Merck Millipore (Darmstadt, Germany). Aminooxy-functionalized tryptophanylarginine methyl ester (aoWR) was prepared as previously described [35 (link)]. Other solvents and reagents were of the highest grade commercially available.
3
Characterization of N-Glycan Profiles
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Approximately 800 ng of gpERAD substrate (6xMyc-mCD3-δ-ΔTM-HA or ATF6α(C)–3xMyc) reacted with 100–150 ng (depending on molecular weight) of α1,2-mannosidase at 37°C for 24 hr were treated with 500 units of PNGase (New England Biolabs) at 37°C overnight. The released N-glycans were captured and labeled with aminooxy-functionalized tryptophanyl arginine methyl ester (aoWR) by BlotGlyco beads (Sumitomo Bakelite) as described previously (Furukawa et al., 2008 (link); Uematsu et al., 2005 (link)). Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analyses of aoWR-labeled glycans were performed on Autoflex Speed (Bruker Daltonics) operated in positive-ion reflector mode. For MS acquisition, aoWR-labeled glycans in acetonitrile were mixed 1:1 with dihydrobenzoic acid (10 mg/ml in 50% acetonitrile) and spotted on the target plate.
4
Efficient N-Glycan Profiling from Cell Lysates
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Whole cell lysates were prepared from MEFs collected by lysing in Tris-buffered saline (TBS) (50 mM tris(hydroxymethyl)aminomethane, 150 mM NaCl, pH 7.4), containing 1% Triton X-100, 1 mM EDTA, and a protease inhibitor cocktail (cOmpleteTM, Roche, Basel, Switzerland) as described previously [104 (link),105 (link)]. N-glycans were released from the whole-cell lysate protein (25 μg) with 2 units of PNGase F (Roche) after reductive alkylation and trypsin digestion. The released N-glycans were captured and labeled with Nα-((aminooxy)acetyl)tryptophanylarginine methyl ester (aoWR) by BlotGlyco beads (Sumitomo Bakelite, Tokyo, Japan) as described previously [106 (link),107 (link)]. After removal of excess reagents by MassPrep HILIC μElution plate, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analyses of aoWR-labeled glycans were performed on Autoflex Speed (Bruker Daltonics, Billerica, MA, USA) operated in positive-ion reflector mode. For MS acquisition, aoWR-labeled glycans in acetonitrile were mixed 1:1 with dihydrobenzoic acid (10 mg/mL in 50% acetonitrile) and spotted on the target plate.
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