I1884

Manufactured by Merck Group

Sourced in United States

I1884 is a laboratory equipment item manufactured by Merck Group. It serves as a core functional component for scientific research and experimentation. The detailed specifications and intended use of this product are not available for this response.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using i1884

Cochlear Basilar Membrane Culture and Nicotine Exposure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cochlear basilar membrane culture was performed as previously described [10 (link)]. Briefly, anesthetized rat pups were decapitated, the cochlea was separated, and placed in Hank’s balanced salt solution under a dissecting microscope. After exposing the membranous labyrinth, the basilar membrane was quickly dissected and transferred to collagen gel-coated culture dishes with Basal Medium Eagle (BME; Sigma-Aldrich, B1522) containing 10 mg/mL bovine serum albumin, 1% serum-free supplement (Sigma-Aldrich, I1884), 2 mM glutamine (Sigma-Aldrich, G6392), 120 mg/mL glucose, and 100 IU/mL penicillin G. The cultures were maintained under 5% (v/v) CO₂ at 37°C in a humidified incubator. After overnight incubation, the cultures were treated with different concentrations of nicotine for 48 h. Nicotine solution was prepared from liquid nicotine (N3876; Sigma-Aldrich, St. Louis, MO, USA) and diluted in culture medium to achieve final concentrations. This solution was prepared daily as needed and the original liquid was kept away from air and light at 4°C

Zhao Y., Liang Y., Pan C., Tang X., Sun Y., Xu C., Sun J, & Sun J. (2021). Nicotine induced ototoxicity in rat cochlear organotypic cultures. Translational Neuroscience, 12(1), 407-414.

+ Open protocol

+ Expand

Cell Culture Conditions for Prostate Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

PC3, DU-145, 22rV1, LAPC4, LNCaP, and THP1 cell lines were cultured in RPMI-1640 (21875-034, Life Technologies) supplemented with 10% fetal bovine serum (FBS-11A, Capricorn Scientific), and 1% penicillin/streptomycin (15140-122, Life Technologies) with 5% CO₂ at 37 °C. LAPC4 was also supplemented with 1 nM dihydrotestosterone (DHT).
The LNCaP-abl cell line was cultured in phenol red-free RPMI-1640 (11835063, Life Technologies) containing 10% CSS (FBS, charcoal-stripped, A3382101, Life Technologies) and 1% penicillin/streptomycin with 5% CO₂ at 37 °C.
VCaP and HEK 293T cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (61965059, Life Technologies) supplemented with 10% FBS and 1% penicillin/streptomycin with 5% CO₂ at 37 °C.
MDA-PCa-2b cell line was cultured in ATCC-formulated F-12K medium (30-2004) supplemented with 20% FBS, 25 ng/ml cholera toxin (C8252, Sigma), 10 ng/ml epidermal growth factor (EGF) (AF-100-15, PeproTech), 0.005 mM phosphoethanolamine (P1348, Sigma), 100 pg/ml hydrocortisone (H0135, Sigma), 45 nM selenium acid (211176, Sigma), 0.005 mg/ml human recombinant insulin (I1884, Sigma), and 1% penicillin/streptomycin with 5% CO₂ at 37 °C.

Bolis M., Bossi D., Vallerga A., Ceserani V., Cavalli M., Impellizzieri D., Di Rito L., Zoni E., Mosole S., Elia A.R., Rinaldi A., Pereira Mestre R., D’Antonio E., Ferrari M., Stoffel F., Jermini F., Gillessen S., Bubendorf L., Schraml P., Calcinotto A., Corey E., Moch H., Spahn M., Thalmann G., Kruithof-de Julio M., Rubin M.A, & Theurillat J.P. (2021). Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nature Communications, 12, 7033.

+ Open protocol

+ Expand

Mouse Follicle Culture and Oocyte Maturation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse follicle culture was conducted as previously reported [21] (link), [22] (link). Briefly, secondary follicles with a diameter of 110–130 µm were isolated from 13-day-old KM female mice and cultured in 96 well plates (single follicle per well) with MEM Glutamax culture medium (a-MEM, 32571; Gibco) with 5% FBS (10108; Gibco), 10 mIU/mL FSH (Serono), 10 mIU/mL LH (Serono), and 5 µl/mL insulin, 5 µl/mL transferrin, and 5 µl/mL selenium (I1884; Sigma). These follicles were exposed to blank (control), propylene glycol (vehicle), or progesterone (either 2 µM or 4 µM) dissolved in propylene glycol. On day 4, 8, and 12, half of the medium was refreshed, and the follicle morphology was analyzed, and the diameters were measured using a microscope. On day 8, the culture medium from 10 follicles in each group was collected for hormone measurement using chemiluminescence (Abbott Biologicals B.V.). On day 12, follicles were stimulated with 1.5 IU/mL hCG (Serono) and 5 ng/mL epidermal growth factor (EGF; 53003; Gibco) at 4:00 PM. On day 13, the maturation of oocytes was assessed: those with polar body extrusion were identified as MII oocytes; those without a polar body but with germinal vesicles broken down were identified as GVBD oocytes; and those without a polar body but with germinal vesicles were identified as germinal-vesicle (GV) oocytes.

Long H., Yu W., Yu S., Yin M., Wu L., Chen Q., Cai R., Suo L., wang L., Lyu Q, & Kuang Y. (2021). Progesterone affects clinic oocyte yields by coordinating with follicle stimulating hormone via PI3K/AKT and MAPK pathways. Journal of Advanced Research, 33, 189-199.

+ Open protocol

+ Expand

Establishment of ccRCC Organoids from Patients

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor tissues from 11 patients with ccRCC were collected to establish ccRCC organoids as described previously with some modifications (59 (link), 60 ). In brief, these tissues were cut into small pieces (1 to 2 mm in size), digested with type II collagenase (17101015, Gibco) for 120 min, and centrifuged at 350 g/s for 5 min at 4°C. The resuspended cells were embedded in 75% (v/v) Matrigel (Corning, NY, USA) solution and cultured with 500 μl of Advanced DMEM/F-12 (Gibco) supplemented with 5% FBS (Gibco), 1× B27 (17504044, Gibco), 10 mM Y-27632 dihydrochloride (M1817, AbMole BioScience, Houston, TX, USA), noggin (100 ng/ml), fibroblast growth factor 2 (20 ng/ml; 100-18B, PeproTech, Rocky Hill, NJ, USA), 1× ITS [insulin (5 μg/ml), transferrin (5 μg/ml), and selenium (5 ng/ml); I1884, Sigma-Aldrich], and 1× penicillin/streptomycin (15140122, Gibco) per well after Matrigel solidifying. The detailed clinical information of the patients was summarized in table S3.

Peng S., Wang Z., Tang P., Wang S., Huang Y., Xie Q., Wang Y., Tan X., Tang T., Yan X., Xu J., Lan W., Wang L., Zhang D., Wang B., Pan T., Qin J., Jiang J, & Liu Q. (2023). PHF8-GLUL axis in lipid deposition and tumor growth of clear cell renal cell carcinoma. Science Advances, 9(31), eadf3566.

+ Open protocol

+ Expand

Chondrogenic Differentiation of BM-MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were transferred to 1 ml eppendorf tubes (50 × 10⁴ cells per tube) and centrifuged at 300 g for five minutes. The resulting cell pellets were statically cultured in DMEM supplemented with 1 mM sodium pyruvate (13360-039, Gibco), 0.1 mM ascorbic acid-2-phosphate (A8960, Sigma-Aldrich), 1% ITS (25 mg/mL insulin, 25 transferrin mg/mL, and 25 ng/mL sodium selenite; I1884, Sigma-Aldrich), 1.25 mg/mL human serum albumin (059487, Octapharma), 500 ng/mL bone morphogenic protein-2 (181720, InductOs) and 10 ng/mL TGFβ1 (240-B, R&D Systems). For controls, the cell pellets were cultured in standard growth medium without stimulation. Chondrogenic medium and standard growth medium were changed twice a week for 21 days in accordance to other studies^{10 (link),55 (link)}. After 21 days all cells were subjected to RNA isolation and RT-qPCR analysis, few samples were stained with Alcian Blue to detect proteoglycans (please see Supplementary Methods for protocol of staining). For positive control of chondrogenic differentiation, the BM-MSCs were stimulated with standard growth medium and chondrogenic medium.

Bogdanova M., Zabirnyk A., Malashicheva A., Enayati K.Z., Karlsen T.A., Kaljusto M.L., Kvitting J.P., Dissen E., Sullivan G.J., Kostareva A., Stensløkken K.O., Rutkovskiy A, & Vaage J. (2019). Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. Scientific Reports, 9, 12934.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!