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Multiscan fc plate reader

Manufactured by Thermo Fisher Scientific
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The Multiscan FC plate reader is a versatile instrument that can be used to measure various types of assays in microplates. It is capable of detecting absorbance, fluorescence, and luminescence signals. The Multiskan FC offers a range of optical configurations to accommodate different applications.

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Lab products found in correlation

Mts reagent, by Promega (7 mentions) 96 well flat bottomed plate, by Corning (7 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions) Elisa kit, by USCN (2 mentions) 2n h2so4, by R&D Systems (2 mentions) Igg total mouse uncoated elisa kit, by Thermo Fisher Scientific (2 mentions) Star117p, by Bio-Rad (2 mentions) Tmb substrate solution, by Thermo Fisher Scientific (2 mentions) Cck 8 kit, by Beyotime (1 mentions) Elisa, by Vector-Best (1 mentions) 24 well plate, by Corning (1 mentions) Cell death detection elisaplus kit, by Roche (1 mentions) Bn 2 equipment, by Siemens (1 mentions) Immulite 2000 analyzer, by Siemens (1 mentions) Dimension rxl equipment, by Siemens (1 mentions) Skanit for multiscan fc software, by Thermo Fisher Scientific (1 mentions) Elisa, by Thermo Fisher Scientific (1 mentions) Elisa kit, by MyBioSource (1 mentions) Foetal calf serum, by Thermo Fisher Scientific (1 mentions) Hek293, by Thermo Fisher Scientific (1 mentions)

32 protocols using multiscan fc plate reader

1

Serum IgG Quantification and Tp2-specific ELISA

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The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer’s instructions. Serum samples were diluted 1:10,000 before addition to ELISA plates. The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
To assess Tp2-specific IgG production, Nunc MaxiSorp™ Flat bottom 96-well ELISA plates were coated with Tp2 monomer (10 µg mL-1, 100 µL per well) at 4°C overnight. After blocking (1xPBS + 0.1% Tween™20 + 1% BSA) for 2h at RT, mouse serum samples (diluted 1:30) were added to each well and incubated with cross-absorbed anti-mouse IgG HRP polyclonal antibody (diluted 1:10,000) (STAR117P, Bio-Rad) for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific) at medium shaking speed. After four washes in 1xPBS with 0.05% Tween™20, plates were developed with TMB substrate solution (Invitrogen) for 20 min at RT. The enzymatic reaction was stopped by adding 2N H2SO4 (R&D Systems) to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
Goh S., Kolakowski J., Holder A., Pfuhl M., Ngugi D., Ballingall K., Tombacz K, & Werling D. (2021). Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle. Frontiers in Immunology, 12, 674484.
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2

ELISA for Mouse IgG and Tp2-specific IgG

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The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer's instructions. Serum samples were diluted 1:10,000 before addition to ELISA plates. The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
To assess Tp2 specific IgG production, Nunc MaxiSorp™ Flat bottom 96-well ELISA plates were coated with Tp2 monomer (10 µg mL -1 , 100 µL per well) at 4°C overnight. After blocking (1xPBS + 0.1% Tween™20 + 1% BSA) for 2h at RT, mouse serum samples (diluted 1:30) were added to each well and incubated with cross-absorbed anti-mouse IgG HRP polyclonal antibody (diluted 1:10,000) (STAR117P, Bio-Rad) for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific) at medium shaking speed. After four washes in 1xPBS with 0.05% Tween™20, plates were developed with TMB substrate solution (Invitrogen) for 20 min at RT.
The enzymatic reaction was stopped by adding 2N H2SO4 (R&D Systems) to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
Goh S., Kolakowski J., Holder A., Pfuhl M., Ngugi D., Ballingall K.T., Tombácz K., & Werling D. (2021). Development of a yeast-based vaccine forTheileria parvainfection in cattle.
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3

Ebselen Inhibition of Trypanothione Synthase

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Under identical assay conditions described in section High-throughput TryS assay, 5 µM Ebselen (IC50 for TbTryS) (stocks prepared at 80 µM in water with DMSO 20% v/v) was pre-incubated for 1 h at 28 °C with: TbTryS (6.1 × 10−6 µmol/min mL; Sample A) or MM (Sample B). Next, MM and TbTryS were added to sample A and B, respectively, and a reaction (Sample C) containing Ebselen (5 µM), TbTryS (6.1 × 10−6 µmol/min mL) and MM was prepared. All samples were incubated for 1 h at 28 °C and then BIOMOL GREENTM reagent was added to detect Pi formation. A620 nm was measured with a MultiScan FC plate reader (Thermo Fisher Scientific). Control samples (TbTryS + MM) lacking Ebselen were run in parallel. All conditions were tested in 6 replicates and data analysed as described in the aforementioned section.
Benítez D., Franco J., Sardi F., Leyva A., Durán R., Choi G., Yang G., Kim T., Kim N., Heo J., Kim K., Lee H., Choi I., Radu C., Shum D., No J.H, & Comini M.A. (2022). Drug-like molecules with anti-trypanothione synthetase activity identified by high throughput screening. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 912-929.
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4

DPPH Radical Scavenging Assay

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The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity was tested as described previously [39 (link)]. The compound solutions (120 µL) were dispensed into wells of a 96-well microplate. The DPPH (Sigma-Aldrich, Germany) was dissolved in MeOH at concentration of 7.5 µM and the solution (30 µL) was added to each well. The concentrations of tested compounds in the mixtures were 10 µM. The mixtures were shaken and left for 30 min. The absorbance of the resulting solutions was measured at λ = 520 nm with a MultiscanFC plate reader (Thermo Fisher Scientific, Waltham, MA, USA). The scavenging of the DPPH radical in comparison with control (MeOH) (%) was calculated for each compound. Ascorbic acid at 10 µM was used as positive control.
Sintsova O., Gladkikh I., Monastyrnaya M., Tabakmakher V., Yurchenko E., Menchinskaya E., Pislyagin E., Andreev Y., Kozlov S., Peigneur S., Tytgat J., Aminin D., Kozlovskaya E, & Leychenko E. (2021). Sea Anemone Kunitz-Type Peptides Demonstrate Neuroprotective Activity in the 6-Hydroxydopamine Induced Neurotoxicity Model. Biomedicines, 9(3), 283.
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5

Assessing Cell Viability with MTT Assay

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MTT assay was used to assess the cell viability after exposure to pesticides.37 After 24‐hour exposure, the medium containing pesticides was removed, and cells were washed three times in PBS and incubated with 100 µL/well MTT (3‐(4,5‐dimethyl‐thiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide) in a concentration of 0.5 mg/mL for 2 hours. The MTT was removed, and 100 µL/well DMSO was added to dissolve the crystals. The plate was shaken at 900 rpm for 1 minute, and absorbance was measured at 550 nm using a Multiscan FC plate reader (Thermo Fisher Scientific, Denmark).
Mathiesen L., Mørck T.A., Poulsen M.S., Nielsen J.K., Mose T., Long M., Bonefeld‐Jørgensen E., Bossi R, & Knudsen L.E. (2020). Placental transfer of pesticides studied in human placental perfusion. Basic & Clinical Pharmacology & Toxicology, 127(6), 505-515.
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6

Measuring Cell Viability in HT1080 Cells

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In these studies, 5,000 HT1080 cells/well were seeded in 96-well plates (Cat. #167008, Thermo Fisher Scientific; Waltham, MA) and incubated for 12 h/37 °C. After the indicated treatment, medium was replaced with fresh serum-free EMEM containing WST-8 reagent (the cell count reagent SF, Cat. #07553, Nacalai Tesque, Kyoto, Japan) and incubated for 1 h at 37 °C. Plates were scanned at 450 nm with a Multiscan FC plate reader (Thermo Fisher Scientific).
Zheng H., Jiang L., Tsuduki T., Conrad M, & Toyokuni S. (2021). Embryonal erythropoiesis and aging exploit ferroptosis. Redox Biology, 48, 102175.
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7

Cell Viability Assay using CCK-8

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Based on protocols of CCK-8 kits (Beyotime Institute of Biotechnology, Shanghai, China), cells were seeded, cultured for 24 h, and further cultured in 100 μL medium with 10 μL CCK-8 reagent. Absorbance at 450 nm at 14, 48 and 72 h was determined using a Multiscan FC plate reader (Thermo Fisher).
Lei J., Chen P., Zhang F., Zhang N., Zhu J., Wang X, & Jiang T. (2021). M2 macrophages‐derived exosomal microRNA-501-3p promotes the progression of lung cancer via targeting WD repeat domain 82. Cancer Cell International, 21, 91.
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8

Crystal Violet Cytotoxicity Assay

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Cells were seeded into the culture plates and treated with PTX (1 µM), Vin (0.1 µM), and Dox (0.5 µM) for 96 hrs. Cell medium was aspirated, and 5 mL of 0.5% crystal violet staining solution was added to each well and incubated for 20 min at room temperature. Plates were washed four times in a stream of tap water and kept inverted on filter paper to air-dry 3 mL of 1% SDS was added to each plate and kept on a shaker at room temperature for 1 h. The optical density was measured at 570 nm wavelength using a MultiScan FC plate reader (Thermo Fisher Scientific, Waltham, MA, USA).
Boichuk S., Bikinieva F., Valeeva E., Dunaev P., Vasileva M., Kopnin P., Mikheeva E., Ivoilova T., Mustafin I, & Galembikova A. (2023). Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 Subline. Diagnostics, 13(16), 2646.
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9

Metabolic Activity Assessment of Larvae

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Metabolic activity of larvae was evaluated by the MTT test, which we initially used to assess the time-dependent effects of SB, SCH and DHSB at concentrations of 5 μM and 50 μM after 4 h, 24 h and 72 h of cultivation. Larvae exposed to FLs were transferred to pre-weighed tubes for hypoxic conditions and to 24-well plates for aerobic cultivation in 1 mL of RPMI medium. 4 h before the selected period of exposure, 40 μL of 0.5 % MTT was added to the tubes/plates. The supernatant was then removed, larvae were washed with PBS and homogenized with a pestle and mortar in 200 μL of DMSO. After centrifugation at 3000 rpm for 10 min, DMSO containing formazan was transferred to a 96-well plate and absorbance was measured at 550 nm with the reference filter at 630 nm using a Multiscan FC Plate Reader (Thermo Scientific, Waltham, MA, USA). The absorbance obtained for the real weight of larval tissue in tubes was re-calculated for 50 mg of tissue/sample, and these values were used to express changes in metabolic activity relative to untreated control samples (100%) for each time point and concentration.
Hrčková G., Kubašková T.M., Benada O., Kofroňová O., Tumová L, & Biedermann D. (2018). Differential Effects of the Flavonolignans Silybin, Silychristin and 2,3-Dehydrosilybin on Mesocestoides vogae Larvae (Cestoda) under Hypoxic and Aerobic In Vitro Conditions. Molecules, 23(11), 2999.
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10

Cytokine-Induced NK Cell Activation

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105 cells of an individual NK cell clone (at week 5 of cultivation) were transferred to a new 96-well round-bottom plate with fresh complete medium for 24 h, then washed and incubated with IL-12 (10 ng/ml) and IL-15 (10 ng/ml) for 18 h. Supernatants were then collected and IFN-γ level was analyzed by ELISA (Vector-Best, Russia). Optical density was measured using Multiscan FC plate reader (Thermo Fisher Scientific, USA) with 450 nm basic filter and 620 nm reference filter. Concentrations were calculated based on standard curves and formulas provided with the kit.
Streltsova M.A., Erokhina S.A., Kanevskiy L.M., Lee D.A., Telford W.G., Sapozhnikov A.M, & Kovalenko E.I. (2018). Analysis of NK cell clones obtained using interleukin-2 and gene-modified K562 cells revealed the ability of “senescent” NK cells to lose CD57 expression and start expressing NKG2A. PLoS ONE, 13(12), e0208469.
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