Log In Sign up
The largest database of trusted experimental protocols

Pathscan enabler 4

Manufactured by Meyer Instruments
Visit Supplier
Sourced in United States

The PathScan Enabler IV is a lab equipment product designed to enable high-throughput, automated Western blot analysis. It features a compact, user-friendly design and is capable of processing multiple samples simultaneously.

Automatically generated - may contain errors

Lab products found in correlation

Leica application suite x software, by Leica Microsystems (4 mentions) Pathscan enabler 5, by Meyer Instruments (2 mentions) Dcf700t, by Leica Microsystems (2 mentions) Dm rb research microscope, by Leica Microsystems (2 mentions) Leica dcf700t, by Leica Microsystems (2 mentions) Lsm 710, by Zeiss (2 mentions) Matlab, by MathWorks (1 mentions) Mabs1251, by Merck Group (1 mentions) Envision flex hrp system, by Agilent Technologies (1 mentions) Dfc310fx, by Leica Microsystems (1 mentions) Dmi6000b, by Leica Microsystems (1 mentions) Microm hm 355, by Thermo Fisher Scientific (1 mentions) Ab32570, by Abcam (1 mentions) Ab125212, by Abcam (1 mentions) Ab68672, by Abcam (1 mentions) Ab8295, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole dapi dye, by Thermo Fisher Scientific (1 mentions) Af835, by R&D Systems (1 mentions) Bright field microscope, by Leica camera (1 mentions) Fluorescent mounting media, by Vector Laboratories (1 mentions)

36 protocols using pathscan enabler 4

1

Quantification of Lacrimal Gland Inflammation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantification of lacrimal gland inflammation by focus scoring was performed as previously described [49 (link)]. Briefly, hematoxylin and eosin (H&E)-stained sections of formalin-fixed, paraffin-embedded, exorbital lacrimal glands were analyzed by standard light microscopy using a 10× objective. Foci composed of a minimum of 50 mononuclear cells were counted and slides scanned to obtain digital images using PathScan Enabler IV (Meyer Instruments, Houston, TX, USA). Tissue areas were measured using ImageJ software [50 (link)] and focus scores quantified as number of inflammatory cell foci per 4 mm2 tissue area. Representative low-magnification digital images were obtained with a PathScan Enabler IV slide scanner (Meyer Instruments).
Chaly Y., Barr J.Y., Sullivan D.A., Thomas H.E., Brodnicki T.C, & Lieberman S.M. (2018). Type I Interferon Signaling Is Required for Dacryoadenitis in the Nonobese Diabetic Mouse Model of Sjögren Syndrome. International Journal of Molecular Sciences, 19(10), 3259.
+ Open protocol
+ Expand
2

Quantifying Myocardial Infarct Size

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Masson’s trichrome-stained sections were used to assess infarct size. Histological sections were scanned with a digital pathology slide scanner (PathScan Enabler IV, Meyer Instruments), and infarct size was measured using MIQuant software as described[25 (link)]. The infarcted LV wall was calculated by the midline length measurement (calculated by dividing the midline length of the infarcted LV wall by the midline length of the total LV wall) as previously validated by Nascimento et al.[25 (link)]. Only regions with infarct in > 50% of the whole thickness of the myocardium were considered for infarct midline[26 (link)].
Bellio M.A., Kanashiro-Takeuchi R.M., Takeuchi L., Kulandavelu S., Lee Y.S., Balkan W., Young K.C., Hare J.M, & Khan A. (2022). Systemic delivery of large-scale manufactured Wharton’s Jelly mesenchymal stem cell-derived extracellular vesicles improves cardiac function after myocardial infarction. The journal of cardiovascular aging, 2, 9.
+ Open protocol
+ Expand
3

Histological Analysis of Harvested Heart Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following DECT imaging, harvested hearts were sectioned for histological analysis. Sections reserved for histology were fixed in 10% buffered formalin, embedded in paraffin, and sliced into five-micron sections. Slices were stained with hematoxylin/eosin (H&E) and Masson’s trichrome. Images were acquired with a PathScan Enabler IV pathology slide scanner (Meyer Instruments). Collagen volume fraction (CVF) was obtained from whole slice images; positively stained pixels were calculated as a fraction of total pixels using available image processing tools (Matlab, The Mathworks, Natick, MA; Fig 1).
Kumar V., McElhanon K.E., Min J.K., He X., Xu Z., Beck E.X., Simonetti O.P., Weisleder N, & Raman S.V. (2017). Non-contrast Estimation of Diffuse Myocardial Fibrosis with Dual Energy CT: A Phantom Study. Journal of cardiovascular computed tomography, 12(1), 74-80.
+ Open protocol
+ Expand
4

Quantifying Pancreatic Acinar Necrosis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As described previously24 (link), whole pancreas H&E-stained sections were examined by a trained morphologist blinded to the sample. Briefly, all pancreatic parenchymal area was imaged using the PathScan Enabler IV slide scanner (Meyer Instruments, Huston, TX) and images were evaluated for acinar necrosis. Necrotic area and total acinar area were measured in pixels for each pancreas. Percentage necrosis was reported as a percentage of total area for each pancreas.
de Oliveira C., Khatua B., El-Kurdi B., Patel K., Mishra V., Navina S., Grim B.J., Gupta S., Belohlavek M., Cherry B., Yarger J., Green M.D, & Singh V.P. (2020). Thermodynamic interference with bile acid demicelleization reduces systemic entry and injury during cholestasis. Scientific Reports, 10, 8462.
+ Open protocol
+ Expand
5

Quantification of Glandular Inflammation

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitation of lacrimal and salivary gland inflammation was performed as previously described [53 (link)]. Briefly, exorbital lacrimal glands and submandibular salivary glands were fixed in formalin, processed, embedded in paraffin, and 5 µm sections were stained with hematoxylin and eosin (H&E). Inflammation was quantified in a blinded manner by standard light microscopy at 10× objective using standard focus-scoring with a focus defined as an aggregate of at least 50 mononuclear cells and the focus score defined as the number of foci per 4 mm2 of tissue. Tissue areas were calculated by ImageJ software [54 (link)] using low magnification digital images obtained by scanning H&E-stained sections with the PathScan Enabler IV (Meyer Instruments, Houston, TX, USA). The WT lacrimal and salivary glands in Figure 1 were previously published in comparison to another KO NOD strain [21 (link)] from the same colony as the Tlr7 KO samples to which they were compared in this study. Representative H&E-stained sections in the figure were obtained by whole-slide scans using the PathScan Enabler 5 (Meyer Instruments).
Debreceni I.L., Chimenti M.S., Serreze D.V., Geurts A.M., Chen Y.G, & Lieberman S.M. (2020). Toll-Like Receptor 7 Is Required for Lacrimal Gland Autoimmunity and Type 1 Diabetes Development in Male Nonobese Diabetic Mice. International Journal of Molecular Sciences, 21(24), 9478.
+ Open protocol
+ Expand
6

Correlating Probe Accumulation with Osteoarthritis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The probes' ability to detect osteoarthritic tissue was verified using histological methods, which involved determining the locations of probe accumulation and FR expression on human osteoarthritic tissue. Human osteoarthritic tissue incubated with probes (100 µg/mL for 2 hours) were cryosectioned into two consecutive sections. One section was used for staining FR expression. The second section was used for NIR imaging to visualize the probe distribution. Subsequently, the slide was stained with Safranin O to assess the severity of cartilage tissue degeneration on the surface of the osteoarthritic cartilage. The Safranin O staining was carried out as described earlier 40 (link). After the staining, the slice was scanned through a pathology slide scanner (PathScan Enabler IV, Meyer Instruments, TX, USA). By merging NIR images and Safranin O images, we would be able to determine whether the probes preferentially accumulated on and diagnosed the location of the degeneration tissue.
Li S., Cong W., Hakamivala A., Huang Y., Borrelli J J.r, & Tang L. (2018). Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage. Nanotheranostics, 2(4), 347-359.
+ Open protocol
+ Expand
7

Immunohistochemical Screening of Adrenal Zones

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies toward steroid or catecholamine synthetic enzymes with adrenal zone-selective expression (Figure 1) were used for IHC to screen candidate adrenals for zone capture. IHC staining was performed using antibodies against CYP11B2 (mouse monoclonal clone 41–17B; 1:1250; Millipore; number MABS1251) (Gomez-Sanchez et al., 2014 (link)), VSNL1 (mouse monoclonal; 1:1000; Millipore; number MABN762), HSD3B2 (mouse monoclonal; 1:5000, kindly provided by Dr. Gomez-Sanchez) (Gomez-Sanchez et al., 2017 (link)), CYB5A (mouse monoclonal; 1:5000; Acris; number AM31963PU-N), and TH (rabbit monoclonal; 1:15,000, kindly provided by Dr. John Porter) (Porter, 1986 (link)) for 60 minutes on FFPE sections. Tissue sections were boiled for 15 min in Tris/EDTA pH 9 antigen retrieval buffer (Vector Laboratories, Burlingame, CA). The FLEX HRP EnVision System (Dako) was used for detection. Diaminobenzidine chromogen was then applied for approximately 45 seconds, 30 seconds, 30 seconds, 30 seconds, and 15 seconds respectively. Slides were counterstained with Harris hematoxylin for 5 seconds and then dehydrated and coverslipped. IHC images were scanned using PathScan Enabler IV (Meyer Instruments, Houston, TX).
Baker J.E., Plaska S.W., Qin Z., Liu C.J., Rege J., Rainey W.E, & Udager A.M. (2021). Targeted RNA sequencing of adrenal zones using immunohistochemistry-guided capture of formalin-fixed paraffin-embedded tissue. Molecular and cellular endocrinology, 530, 111296.
+ Open protocol
+ Expand
8

Perfusion-Fixed Brain Tissue Sectioning

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals were euthanized under anesthesia, and then perfused transcardially with 200 ml normal saline followed by 200 ml ice-cold 4% paraformaldehyde for fixation. Brains were stored in 4% paraformaldehyde overnight at 4 °C, then transferred to a 30% sucrose solution for 3 days for cryoprotection. Brains were then sectioned coronally (40 µm) on a freezing microtome with approximately 500 µm distance between slices. Sections were mounted onto gelatin coated slides, dried overnight and stained with 0.2% cresyl violet for measurement of brain tissue loss. The stained sections were photographed using a digital pathology slide scanner (PathScan Enabler IV, Meyer Instruments, USA).
Chiluwal A., Narayan R.K., Chaung W., Mehan N., Wang P., Bouton C.E., Golanov E.V, & Li C. (2017). Neuroprotective Effects of Trigeminal Nerve Stimulation in Severe Traumatic Brain Injury. Scientific Reports, 7, 6792.
+ Open protocol
+ Expand
9

Histopathological Examination of Infected Eyes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following infection and drug treatment, eyes were enucleated and fixed in 4% formalin for histopathological examination. The embedding, sectioning, and hematoxylin & eosin (H&E) staining was performed by Excalibur Pathology, Inc. (Oklahoma City, OK, USA). Pathscan Enabler IV (Meyer Instruments, Inc.) was used to scan H&E stained slides.
Singh S., Singh P.K., Jha A., Naik P., Joseph J., Giri S, & Kumar A. (2021). Integrative metabolomics and transcriptomics identifies itaconate as an adjunct therapy to treat ocular bacterial infection. Cell Reports Medicine, 2(5), 100277.
+ Open protocol
+ Expand
10

Quantification of Glandular Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Exorbital lacrimal and submandibular salivary glands were harvested and fixed in buffered formalin, processed, embedded in paraffin, and sectioned. Five micrometer sections of paired glands were stained with hematoxylin and eosin (H&E) and analyzed by standard light microscopy. Inflammation was quantified using standard focus scoring57 (link). Focus scores (number of inflammatory foci per 4 mm2) were calculated by a blinded observer by counting the total number of foci (composed of ≥ 50 mononuclear cells) by standard light microscopy using a 10× objective, scanning slides to obtain digital images using PathScan Enabler IV (Meyer Instruments), and measuring surface area of sections using ImageJ software58 (link). Samples with diffuse inflammation resulting in coalescence of individual foci were assigned focus score values greater than the highest calculable value for that set of comparisons. Representative images were captured on a Leitz DM-RB research microscope with a Leica DCF700T digital camera using Leica Application Suite X software (Leica Microsystems, Wetzlar, Germany).
Barr J.Y., Wang X., Meyerholz D.K, & Lieberman S.M. (2017). CD8 T cells contribute to lacrimal gland pathology in the nonobese diabetic mouse model of Sjögren syndrome. Immunology and cell biology, 95(8), 684-694.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!