Ivis spectrum ct pre clinical

Mice were imaged using an IVIS Spectrum CT Pre-clinical in-vivo imaging system (PerkinElmer, MA, USA) with the following parameters: CT was set to 50ms, total projections were 720, voltage was set to 50kV while the current to 1mA the binning was determined to 2 and the X-ray filter was set to 440 A1. Hounsfield calibration was performed in air-0.004168 and water-0.5513. Two groups of tumors bearing mice (n=5 each) were divided to control and test group. The mice were weighed weekly and tumor progression was monitored by weekly IVIS scans (PerkinElmer). Epi-fluoresce scans were acquired under the following parameters: excitation 570nm, emission 620nm, at 3 seconds exposure. Therapeutic treatment began four weeks after the initial tumor cells injection. The test group received 300μl dose of collagozomes for two consecutive days, while the control group remained untreated. On the third day, both groups received a single dose of 10 mg/kg micellar paclitaxel.^{19 (link)} Tumor dimensions were assessed using the ROI tool in the IVIS imaging software (Living Image, PerkinElmer).