Apc conjugated cd90 antibody

Manufactured by BD

Sourced in United States

The APC-conjugated CD90 antibody is a laboratory reagent used for the identification and analysis of CD90-positive cells in flow cytometry applications. It provides a direct fluorescent label for the detection of CD90, a cell surface glycoprotein, without the need for additional labeling steps.

Automatically generated - may contain errors

Lab products found in correlation

Pkh26 red fluorescent cell linker kit, by Merck Group (1 mentions) Fitc conjugated cd44 antibody, by BD (1 mentions) Fitc conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Cy3 conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Pcmv neo bam p53 r249s, by Addgene (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Cisplatin, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) H1299, by American Type Culture Collection (1 mentions) Pe conjugated cd31 antibody, by BD (1 mentions) Astrios sorter, by BD (1 mentions)

2 protocols using apc conjugated cd90 antibody

Lung Cancer Cell Line Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

A549, H460, H661, and H1299 lung cancer cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured with RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Hyclone, South Logan, UT, USA). Cisplatin, 4′,6-diamidino-2-phenylindole (DAPI), and a PKH-26 Red Fluorescent Cell Linker Kit were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). FITC-conjugated CD44 antibody, APC-conjugated CD90 antibody, and corresponding isotype controls were purchased from BD Biosciences (San Jose, CA, USA). Anti-human CD44, CD90, and p53 primary antibodies were purchased from Abcam (Cambridge, MA, USA). CCR2 antibody was obtained from R&D (Minneapolis, MN, USA); CD68 antibody and Lipofectamine 2000 reagent were purchased from Invitrogen (Carlsbad, CA, USA). Secondary antibodies FITC-conjugated goat anti-rabbit IgG and Cy3-conjugated goat anti-mouse IgG were purchased from Jackson ImmunoResearch (West Grove, PA, USA). pCMV-Neo-Bam p53 R249S was a gift from Bert Vogelstein (Addgene plasmid # 16438), and pCMV-Neo-Bam p53 wt (wt p53) was a gift from Bert Vogelstein (Addgene plasmid # 16434).

Xu Y., Xu Z., Li Q., Guo L., Wang Y., Zhou J., Wang G, & Liu Y. (2019). Mutated p53 Promotes the Symmetric Self-Renewal of Cisplatin-Resistant Lung Cancer Stem-Like Cells and Inhibits the Recruitment of Macrophages. Journal of Immunology Research, 2019, 7478538.

+ Open protocol

+ Expand

Isolation and Characterization of Neonatal Rat Cardiac Cells

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cardiac cells were isolated from the ventricles of 2-day old neonatal rat hearts according to previously described methods [25 (link), 28 (link)]. Animals were sacrificed using humane standards approved by the Institutional Animal Care and Use Committee (IACUC) of Duke University. Ventricles were harvested, minced, and enzymatically dissociated by serial digestion with trypsin and collagenase. Isolated cells were resuspended in DMEM/F-12 with 10% Fetal Bovine Serum and 10% Horse Serum, and pre-plated for 45 min at 37 °C to increase the fraction of cardiomyocytes in the cell suspension [28 (link), 29 (link)]. To assess cardiomyocyte purity, pre-plated cells were fixed and permeabilized according to the manufacturer instructions (BD Biosciences # 554714), then stained with cardiac troponin T (cTnT) primary antibody (Abcam ab45932) and Alexa Fluor-conjugated secondary antibody. To assess fraction of endothelial cells, pre-plated cells were washed with PBS and stained with PE-conjugated CD31 antibody (BD Biosciences #555027) and APC-conjugated CD90 antibody (BD Biosciences #561409). Stained cells were analyzed in Beckman Astrios Sorter and BD FACSCanto A analyzer at the Duke Cancer Institute’s Flow Cytometry Shared Resource. Endothelial cells and fibroblasts were identified as CD31⁺ and CD90⁺ /CD31⁻ [30 (link)] cells, respectively.

Jackman C.P., Ganapathi A.M., Asfour H., Qian Y., Allen B.W., Li Y, & Bursac N. (2018). Engineered cardiac tissue patch maintains structural and electrical properties after epicardial implantation. Biomaterials, 159, 48-58.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!