Thalli of Chara australis R.Br. used for microscopic investigation were grown as described in Schmoelzer et al. (2011 (link)). Internodal cells were isolated from the main axis with a small pair of scissors and left in artificial freshwater (AFW; 10−3 M NaCl, 10−4 M KCl, 10−4 M CaCl2) until use. For visualization of the pH in the external medium along the cell surface, 50 μM phenol red (phenolsulfonphthalein; Sigma-Aldrich, St. Louis, USA) was added to the AFW or to AFW containing 1.5 × 10−4 M NaHCO3.
Phenolsulfonphthalein
Manufactured by Merck Group
Sourced in United States
Phenolsulfonphthalein is a laboratory chemical used as a pH indicator. It displays a range of colors depending on the pH of the solution it is added to, allowing for the determination of acidity or basicity.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions)
Phenol red, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nf κb p50 p65 transcription factor assay kit, by Abcam (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Hank s buffered salt solution, by Thermo Fisher Scientific (1 mentions)
5 chloromethylfluorescein diacetate cmfda, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid, by Thermo Fisher Scientific (1 mentions)
Milli q plus water, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Mk 571, by Enzo Life Sciences (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Aniline, by Merck Group (1 mentions)
Dm2700m, by Leica (1 mentions)
Pli 100a, by Warner Instruments (1 mentions)
5 protocols using phenolsulfonphthalein
1
Microscopic Investigation of Chara australis
2
In Vitro Barrier Dysfunction Assessment
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Dulbecco’s modified Eagle’s medium (DMEM), non-essential amino acids (NEAA), and fetal bovine serum (FBS) were obtained from GIBCO Laboratories (Grand Island, NY, United States). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenolsulfonphthalein were obtained from Sigma-Aldrich (St. Louis, MO, United States). Fluoroisothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) were purchased from Lianke Biotechnology Co (Hangzhou, China). Antibodies specific for claudin 1, claudin 2, zo3, occludin, MLC (phospho S20), MLC (pan), and MLCK and the NF-κB p50/p65 transcription factor assay kit were all purchased from Abcam (Cambridge, MA, United States). Enzyme-linked immunosorbent (ELISA) kits for TNF-α, IL-6 and IL-8 were obtained from eBioscience (San Diego, CA, United States).
3
Quantification of Intracellular MTX and its Transporters
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Indican, indoxyl sulfate (IS) and phenolsulfonphthalein (PSP) were obtained from Sigma–Aldrich Chemical Co. (St. Louis, MO, U.S.A.). Methotrexate (MTX) (25.0 mg/mL) was obtained from Wyeth Pharma Gmbh (Wolfratshausen, Germany). MK 571 (purity 98%) was obtained from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). Dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), 3-(4′,5′-dimethylthiazol-2′-yl)-2,5-diphenyltetrazolium bromide (MTT) and triton X-100 were supplied by Sigma (St. Louis, MO, USA). Fetal Bovine Serum (FBS) was obtained from Biological Industries Inc. (Kibbutz, Beit Haemek, Israel). Penicillin-Streptomycin-Glutamine, Dulbecco’s Modified Eagle Medium (DMEM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Hank’s Buffered Salt Solution (HBSS), 5-chloromethylfluorescein diacetate (CMFDA) and trypsin/EDTA were purchased from Invitrogen (Grand Island, NY, USA). TDx kit of MTX was purchased from Abbott Laboratories (Abbott Park, IL, USA). Other reagents were HPLC grade or reagent grade. Milli-Q plus water (Millipore, Bedford, MA, USA) was used throughout this study.
4
Silane-Functionalized Aniline Protocol
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Aniline (ANI), N-[3-(trimethoxysilyl)propyl]Aniline (APTMS), diisopropylethylamine
(DIEA), ammonium persulfate, and phenol red also known as phenolsulfonphthalein
(PSP) were supplied by Sigma-Aldrich. All solvents used were of analytical
grade, and all solutions were made with Milli-Q water of 18 MΩ·cm
resistivity.
(DIEA), ammonium persulfate, and phenol red also known as phenolsulfonphthalein
(PSP) were supplied by Sigma-Aldrich. All solvents used were of analytical
grade, and all solutions were made with Milli-Q water of 18 MΩ·cm
resistivity.
5
Zebrafish microRNA Modulation Assay
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Adult zebrafish were mated and spawned for 10e15 min, and the fertilized eggs were quickly collected for microinjection. MicroRNA mimics and inhibitors for miR-96 and miR-184 were synthesized by GenePharma (Shanghai, China) and used as received. Concentrations of mimics or inhibitors were adjusted to 200 ng mL À1 and 2 nL of this solution were injected into each egg (Pli-100A, Warner Instruments, Hamden, USA), and equal amount of 0.1% phenolsulfonphthalein (Sigma, St. Louis, USA) solution was injected as the negative control group; any dead embryos were removed every 12 h.
Thirty embryos or larvae from each treatment were randomly selected and anesthetized with 0.03% tricaine methanesulfonate (buffered MS-222, Sigma, St. Louis, USA) for 30 s before observation using an optical microscope (DM2700 M, Leica, Heidelberg, Germany).
Thirty embryos or larvae from each treatment were randomly selected and anesthetized with 0.03% tricaine methanesulfonate (buffered MS-222, Sigma, St. Louis, USA) for 30 s before observation using an optical microscope (DM2700 M, Leica, Heidelberg, Germany).
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