Beckman fc500

Manufactured by Beckman Coulter

Sourced in United States

The Beckman FC500 is a flow cytometer designed for high-performance cell analysis. It is equipped with multiple lasers and detectors to measure various parameters of cells or particles in a fluid sample.

Automatically generated - may contain errors

Lab products found in correlation

Annexin 5 fitc pi apoptosis detection kit, by Merck Group (3 mentions) Fitc annexin 5 apoptosis detection kit, by BD (3 mentions) Edta free trypsin, by Thermo Fisher Scientific (1 mentions) Annexin 5 fitc apoptosis kit, by BestBio (1 mentions) Hoechst 33258, by Solarbio (1 mentions) Anti il 17a pe, by BD (1 mentions) Leukocyte activation cocktail, by BD (1 mentions) Anti cd4 fitc, by BD (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Yeasen (1 mentions) Alexa fluor 488, by Cell Signaling Technology (1 mentions) Wincycle software, by Beckman Coulter (1 mentions) Paraformaldehyde (pfa), by Solarbio (1 mentions) Annexin 5 fitc pi kit, by BestBio (1 mentions) Cell death detection elisa kit, by Roche (1 mentions) Cell cycle kit, by BestBio (1 mentions) Annexin 5 fitc pi apoptosis kit, by BestBio (1 mentions)

20 protocols using beckman fc500

Apoptosis and Cell Cycle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Apoptosis assay was performed by fluorescein isothiocyanate-conjugated Annexin V (Annexin V-FICT) and propidium iodide (PI) double staining after cell culture for five days, when a confluence of 80% or more was achieved. The cells were harvested with 0.25% trypsin, washed with PBS and stained with Annexin V-FITC/PI apoptosis kit (Bestbio, China) according to the manufacturer’s instructions and analyzed by flow cytometry (Beckman FC500, USA).
Cells for cell cycle assay were cultured in RPMI serum-free medium for 24 hrs. The medium was changed to RPMI with 10% fetal bovine serum and the cells were cultured for 24 hrs. Cells were collected, fixed in ice-cold 75% ethanol and stored in −20°C for one hour. The fixed cells were washed with PBS, treated using cell cycle kit (Bestbio, China) according to the manufacturer’s instructions and analyzed by flow cytometry (Beckman FC500, USA).

Peng H.S., Liao M.B., Zhang M.Y., Xie Y., Xu L., Zhang Y.J., Zheng X.F., Wang H.Y, & Chen Y.F. (2014). Synergistic Inhibitory Effect of Hyperbaric Oxygen Combined with Sorafenib on Hepatoma Cells. PLoS ONE, 9(6), e100814.

+ Open protocol

+ Expand

Quantifying Cell Apoptosis via Annexin V

Check if the same lab product or an alternative is used in the 5 most similar protocols

Annexin V (FITC) Apoptosis Detection kit (BD Biosciences, San Diego, CA, USA) was recruited to test cell apoptosis. After 48 hrs of lentivirus infection, NCI-N87 and MKN28 cells were collected with 0.25% of EDTA‑free trypsin (Thermo Fisher Scientific) and washed with PBS for one time. Subsequently, the cells were incubated with 100 μL of 1X binding buffer solution containing 5 μL of Annexin V and 5 μL PI solution for 15 mins in the dark. Then, the cells were washed with 1X of binding buffer for three times and resuspended with 500 μL of 1X binding buffer. Cell apoptosis was detected with a Beckman FC500 flow cytometer (Beckman Coulter, Inc., Brea, California, USA) and analyzed by FlowJo 7.6 software.

Chen W., Wu G., Zhu Y., Zhang W., Zhang H., Zhou Y, & Sun P. (2019). HOXA10 deteriorates gastric cancer through activating JAK1/STAT3 signaling pathway. Cancer Management and Research, 11, 6625-6635.

+ Open protocol

+ Expand

Annexin V-FITC/PI Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell apoptosis was analyzed by Annexin V-FITC/PI Apoptosis Detection Kit according to the manufacturer's protocol (Sigma-Aldrich Co., St. Louis, MO). Briefly, cells (H1650, A549, and PC9) were seeded in 6-well plates. After 24 h of culture, cells were treated with increased doses of FZKA and then incubated at 37°C for 24 h. Afterwards, cells were collected, centrifuged for 5 min at 1500 rpm, and resuspended in 1x binding buffer. Finally, 5 μL Annexin V-FITC and 5 μL PI were added to the cells at room temperature for 15 min. The cells were then analyzed using flow cytometer (Beckman FC 500, Beckman Coulter, Inc., CA, USA).

Wang S., Long S., Xiao S., Wu W, & Hann S.S. (2018). Decoction of Chinese Herbal Medicine Fuzheng Kang-Ai Induces Lung Cancer Cell Apoptosis via STAT3/Bcl-2/Caspase-3 Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 8567905.

+ Open protocol

+ Expand

RABV-Induced Apoptosis Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

NA cells were infected with RABV at an MOI of 3 and harvested at 24 hpi. Cells were stained with the Annexin V-FITC apoptosis kit (BestBio, Shanghai, China) according to the manufacturer’s protocols. Flow cytometry was performed on a Beckman FC500 flow cytometer (Beckman Coulter, Fullerton, CA, USA). Data were analyzed using CXP Software.

Luo J., Zhang Y., Zhang Q., Wu Y., Zhang B., Mo M., Tian Q., Zhao J., Mei M, & Guo X. (2019). The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication. Viruses, 12(1), 4.

+ Open protocol

+ Expand

Annexin V-FITC/PI Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell apoptosis was performed using Annexin V-FITC/PI apoptosis detection kit according to the manufacturer’s protocol (Sigma-Aldrich Co. St. Louis, MO), as previously performed [13 ]. In brief, NSCLC cells (A549 and PC9) were grown in 6-well plates. After 24 h of culture, cells were treated with FZKA and Gefitinib in different groups and then incubated at 37 °C for 24 h. Afterwards, cells were collected, centrifuged for 5 min at 1500 rpm, and resuspended in 1× binding buffer. Finally, 5 μL Annexin V-FITC and 5 μL PI were added into the cells at room temperature for 15 min. The cells were then analyzed using flow cytometer (Beckman FC 500, Beckman Coulter, Inc, CA, USA).

Wang S., Peng Z., Li W., Long S., Xiao S, & Wu W. (2020). Fuzheng Kang-Ai decoction enhances the effect of Gefitinib-induced cell apoptosis in lung cancer through mitochondrial pathway. Cancer Cell International, 20, 185.

+ Open protocol

+ Expand

Apoptosis Detection and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

An Annexin V‐FITC/PI apoptosis detection kit (Catalog: G003‐1–3) was used to detect cell apoptosis. After the experimental procedure, the cells of the different treatment groups were collected and washed three times with PBS. Then, the cells were resuspended with a prepared binding buffer (100 μL) and adjusted to a density of 1 × 10⁶ /ml. 100 μL of cell suspension was inoculated into a 5‐mL flow tube and stained with 5 μL Annexin V‐FITC and 5 μL PI that incubate away from light at room temperature for 15 min. Finally, the apoptosis rate was analyzed by flow cytometry (Beckman FC500, Beckman Coulter, Inc.).
Hoechst‐33258 (Beijing Solarbio Science & Technology, Beijing, China, Catalog: IH0060) is a specific DNA dye that can detect cell apoptosis through fluorescence intensity. After the experimental procedure, the cell culture medium of 24‐well plates in each group was discarded, and H9c2 cardiomyocytes were washed three times with PBS. Then, 500 μL/well of Hoechst‐33258 staining solution (10 μg/mL) was added to each group of cells and incubated at 37 ℃ for 20 min. Next, the dye was removed, and the cells were rinsed twice with PBS, and a fluorescence microscope was used for fluorescence image collection. Image‐Pro Plus 6.0 software was used for quantification.

Zheng B., Qi J., Liu P., Zhang M., Zhang Y., Xue Y., Han X., Xu S, & Chu L. (2021). 10‐Gingerol alleviates hypoxia/reoxygenation‐induced cardiomyocyte injury through inhibition of the Wnt5a/Frizzled‐2 pathway. Food Science & Nutrition, 9(7), 3917-3931.

+ Open protocol

+ Expand

Apoptosis Analysis by Flow Cytometry

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed as described previously (Wang et al., 2018 (link)). Cell apoptosis was analyzed by Annexin V-FITC/PI apoptosis detection kit according to the manufacturer’s protocol (Sigma-Aldrich Co. St. Louis, MO). Briefly, cells (CNE-2, H1299, and HSF) were seeded in 6-well plates. After 24 h of culture, cells were treated with CKI, IR, or IR combined with CKI, and then incubated at 37°C for 24 h. Afterwards, cells were collected, centrifuged for 5 min at 1,500 rpm, and resuspended in 1 × binding buffer. Finally, 5 μL Annexin V-FITC and 5 μL PI were added into the cells at room temperature for 15 min. The cells were then analyzed using flow cytometer (Beckman FC 500, Beckman Coulter, Inc., CA, United States).

Zheng J., Li G., Wang J., Wang S., Tang Q., Sheng H., Wu W, & Wang S. (2021). Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim. Frontiers in Pharmacology, 12, 753068.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Cell Cycle and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were quantified using a Cell Cycle and Apoptosis Analysis kit (cat. no. C1052; Beyotime Institute of Biotechnology), according to the manufacturer's protocol. MC-4 cells were pretreated with pPeOp (30, 60 or 90 µg/ml), 90 µg/ml PVP or 100 µg/ml 5-FU prior to culturing at 37°C for 24 h in a humidified atmosphere containing 5% CO2. The cells were subsequently washed with phosphate-buffered saline (PBS) three times and fixed with 70% ice-cold ethanol at 4°C overnight. The cells were centrifuged, washed with PBS, treated with 20 µl RNase A in a water bath at 37°C for 30 min, and placed in the dark at 4°C for 30 min with 300–500 µl propidium iodide solution. The cell cycle distribution of the cells was determined using a Beckman FC500 flow cytometer (Beckman Coulter, Inc., Brea, CA, USA) with red fluorescent light at a wavelength of 488 nm.

Yang Y.L., Gong W.Y., Chen F.F., Chen L.C, & Chen Y.T. (2017). pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC-4 gastric tumor cells. Oncology Letters, 14(1), 533-540.

+ Open protocol

+ Expand

Phenotyping Cell Differentiation via Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

In order to determine the different phenotypes of cell differentiation, flow cytometry was used. PBMCs and splenocytes from CIA mice, as well as cultured Th17 cells in vitro, were collected. Cells were then washed twice with PBS and activated with leukocyte activation cocktail (BD Pharmingen) for 4 h. After staining of Th17 cells with anti-CD4-FITC (BD Pharmingen) and anti-IL-17A-PE (BD Pharmingen) for 30 min, intracellular cytokines were analyzed by flow cytometry (Beckman FC-500, Beckman Coulter, Inc., Brea, CA, USA).

Di Y., Zhang M., Chen Y., Sun R., Shen M., Tian F., Yang P., Qian F, & Zhou L. (2021). Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels. Yonsei Medical Journal, 63(1), 56-65.

+ Open protocol

+ Expand

Apoptosis Evaluation of C. albicans

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed using the Annexin V-FITC/PI Apoptosis Detection Kit (Yeason, Beijing, China). After the treatment of 15 μg/mL EVs and PBS control at 37 °C for 6 h, C. albicans was centrifuged at 300 g for 5 min at 4 °C. The precipitate was washed twice with pre-cooled PBS. After the centrifugation to collect the precipitate, PBS was discarded, and then resuspended with 100 μL binding buffer. Then, 5 μL Annexin V-FIFC and 10 μL PI were added and the samples were mixed well. After the reaction at room temperature for 10–15 min in the dark, 400 μL binding buffer was added. A flow cytometer (Beckman FC500, Carlsbad, CA, USA) was used for analysis, and the excitation and emission wavelengths were set to 488 and 525 nm, respectively. All experiments were performed in triplicate.

Wei Y., Wang Z., Liu Y., Liao B., Zong Y., Shi Y., Liao M., Wang J., Zhou X., Cheng L, & Ren B. (2022). Extracellular vesicles of Candida albicans regulate its own growth through the l-arginine/nitric oxide pathway. Applied Microbiology and Biotechnology, 107(1), 355-367.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!