Embryos were dechorionated and placed in halocarbon oil (H8898, Sigma) to prevent dehydration during long-term recordings. Videos were recorded by a stereomicroscope (SZX16, Olympus, Japan) equipped with an XCD-V60 CCD camera Newly hatched larvae were gently washed in distilled water and then placed on an apple-juice agar plate. After a 3-minute acclimation, the crawling movements were recorded for 1 minute by a stereomicroscope (SZX16, Olympus, Japan) equipped with an XCD-V60 CCD camera (30 frames/sec). The number of larval peristalses (sequential muscular contractions across all segments) was manually counted by using ImageJ software (National Institute of Health). Stride duration was calculated as the average time needed for accomplishing a single forward peristalsis.
Szx16
Manufactured by Olympus
Sourced in Japan, United States, Germany, China
The SZX16 is a stereo microscope designed for a variety of laboratory and industrial applications. It features a zoom range of 6.3x to 100x and provides high-resolution, detailed observations. The SZX16 is equipped with a dual-LED illumination system for optimal sample visibility.
Automatically generated - may contain errors
Lab products found in correlation
Dp71 camera, by Olympus (8 mentions)
Lsm 880, by Zeiss (8 mentions)
Szx16 stereomicroscope, by Olympus (5 mentions)
Vhx 5000, by Keyence (5 mentions)
Fv1000, by Olympus (5 mentions)
Su8010, by Hitachi (4 mentions)
U lh100hg, by Olympus (4 mentions)
Leica rm2265, by Leica Microsystems (4 mentions)
Powershot g12, by Olympus (4 mentions)
Focus software, by Helicon (4 mentions)
Bx53 compound microscope, by Olympus (4 mentions)
Cellsens software, by Olympus (4 mentions)
Sigma 300, by Zeiss (4 mentions)
Lsm 780, by Zeiss (4 mentions)
Lsm 710, by Zeiss (4 mentions)
Cellsens standard software, by Olympus (4 mentions)
Axiovert 200m, by Zeiss (4 mentions)
Lsm 700, by Zeiss (4 mentions)
Jsm 6610lv, by JEOL (4 mentions)
Ckx41, by Olympus (3 mentions)
709 protocols using szx16
1
Larval Crawling Analysis Pipeline
2
Chimeric Embryo Generation via EGFP-cviTSCs and cviXENCs
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After culturing for 15 passages, EGFP-cviTSCs were collected. For 4-cell-embryo injection, these cells were injected into ICR 4-cell-embryo with 5–8 cells. The embryos were cultured in vitro until the blastocyst stage. For blastocyst injection, EGFP-cviTSCs were microinjected into ICR blastocysts with 9–15 cells in one blastocyst. Blastocysts were transferred into pseudopregnant ICR females. After 9–10 days, the embryos and placentas were separated from uterus. The injected cells were traced under fluorescent a microscope (Olympus SZX16).
cviXENCs were labeled with red fluorescence by transfecting with vector FUW-DsRed. The process for blastocyst injection was the same with that described above. After transplanting them into pseudopregnant mice for 4 and 5 days, embryos were collected and observed under fluorescent microscopes (Olympus SZX16 and Olympus X51).
cviXENCs were labeled with red fluorescence by transfecting with vector FUW-DsRed. The process for blastocyst injection was the same with that described above. After transplanting them into pseudopregnant mice for 4 and 5 days, embryos were collected and observed under fluorescent microscopes (Olympus SZX16 and Olympus X51).
3
Fixation and Dissection of Staged Rat Embryos
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Staged rat embryos were obtained from timed pregnancies. The whole embryo/fetus was fixed in 4% paraformaldehyde/0.1 M phosphate buffered saline (PBS, pH 7.4) at 4°C for 3 hr and immersed in PBS or 18% sucrose/PBS. Images of the embryos and microdissected fetal organs (CNS and enteric gut) were obtained using a fluorescent stereoscopic microscope (model SZX16, Olympus, Japan). Hindbrains from embryos were dissected, and then the dorsal midline was cut to make an open-book preparation, as described previously [45 (link)]. The dissected hindbrain was placed on a glass slide and flattened by covering the ventricular surface with a cover slip for examination under a microscope (models BX51 and SZX16, Olympus).
4
Zebrafish Skeletal Morphology Analysis
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Morphological analysis of zebrafish larvae at 4 dpf was performed by using a stereoscopic microscope (Olympus SZX16, Tokyo, Japan). Whole-mount skeletal staining of zebrafish larvae at 5 dpf was conducted with Alcian blue staining (Sigma Chemical, Co., St Louis, MO, United States), as described by others (Neuhauss et al., 1996 (link); Walker and Kimmel, 2007 (link)), and was visualized by a stereoscopic microscope (Olympus SZX16, Tokyo, Japan) as previously described (Carvan et al., 2004 (link)).
5
Baculovirus Infection in Silkworm
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As previously described when the Bombyx mori larvae reached the fifth instar stage, they were injected with 5 uL (~1 × 106 VG) each of two the recombinant baculoviruses (or sterile PBS buffer as uninfected control) near the proleg (forward from the body cavity) [15 (link)]. For the observation of pathological symptoms after the injection, the larvae were narcotized on ice, and low-magnification imaging was carried out by stereomicroscope (Olympus SZX16, Shinjuku-ku, Tokyo, Japan) to obtain the whole-larvae fluorescence images. Infected larvae were washed three times with PBS buffer (pH 7.4, Gibco, Grand Island, NY, USA) to collect and stored at −80 °C.
Like the larvae, pupae were injected with 5 µL (~1 × 106 VG) each of two recombinant baculoviruses (or sterile PBS buffer as uninfected control). For the observation of pathological symptoms after the injection, low-magnification imaging was carried out by stereomicroscope (Olympus SZX16, Shinjuku-ku, Tokyo, Japan) to obtain the whole-pupae fluorescence image. Infected pupae were stored at −80 °C to use.
Like the larvae, pupae were injected with 5 µL (~1 × 106 VG) each of two recombinant baculoviruses (or sterile PBS buffer as uninfected control). For the observation of pathological symptoms after the injection, low-magnification imaging was carried out by stereomicroscope (Olympus SZX16, Shinjuku-ku, Tokyo, Japan) to obtain the whole-pupae fluorescence image. Infected pupae were stored at −80 °C to use.
6
Nematode Isolation from Beetle Trachea
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50 ml Erlenmeyer flasks containing B. cinerea growing in autoclaved (121°C, 30 min) barley medium (10 g barley in 15 ml water) for 2 weeks at 25°C were inoculated with 1000 propagative nematodes. After the nematodes had fed for 5 days, autoclaved pine wood sawdust layer was spread on top of the barley layer of the rearing flasks and cultured in 4°C for another 2 weeks. When the color of the pupae’s eyes turned dark, they were put on the nematode-infected sawdust layer until 6 days after eclosion. Emerged adult beetles were dissected by ventral filleting, and the tracheal tubes located on both sides of the thorax were dissected and carefully deprived of surrounded muscle under a stereomicroscope (Olympus SZX16, Japan). The excised tracheal tubes and the left part of the body were washed in PBST (Solarbio, P1033) to irrigate the nematodes and presence of nematode was confirmed under a stereomicroscope (Olympus SZX16, Japan). The total number of isolated nematodes per beetle was counted in plates with an inverted microscope (Olympus CKX41, Japan).
7
Microscopic Imaging Techniques Protocol
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Images were taken using microscopes (BX51 and SZX16, Olympus) equipped with a CCD-camera (DP71, Olympus), a confocal laser microscope (FV1000, Olympus), a virtual slide microscope (VS120-L100, Olympus) and a microscope (SZX16, Olympus) equipped with an HD-color camera (CSD240, Ikeda) and a recorder (VISK IR-100, Chunichi Denshi).
8
Dry Seed and Seedling Phenotyping
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The image displaying the dry seed phenotype was acquired via stereomicroscopy (OLYMPUS, SZX16). The images displaying the seedling phenotype were acquired with a digital camera (CANON 600D). The seed length and width were measured using the “measuring tool” of the incident software of OLYMPUS SZX16. The hundred-grain weight was measured with an analytical balance (OHAUS, AR224CN).
9
Borneol Toxicity in Zebrafish Embryos
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At 24 hpe (hours post-exposure), Borneol-/VHC-exposed zebrafish were anesthetized in tricaine 20 (link) and positioned in 0.1% regular agarose G-10 (111,860, Biowest, Shanghai, China) in Milli-Q water for examination using a stereomicroscope (Olympus SZX16, Tokyo, Japan) with cellSens Standard software 2.2. Dead fish were recorded (defining with heart beating) and LC 10 and LC 50 were calculated by using a dose-mortality curve. Four borneol concentrations (200, 300, 400, and 500 μM) below LC 10 were used for zebrafish intoxication from 3 dpf. And the morphology of embryos (including pericardium, swimming bladder, body shape, and yolk absorption) was examined every 24 h between 24 and 72 hpe using an Olympus SZX16 microscope as described above.
10
Quantifying Cell Death and Oxidative Stress
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Dye exclusion experiments were performed with fully expanded leaves from 24-day-old plants, for 20 min with immersion treatment or for 2 h with 5 µl droplets of a 0.05% solution of toluidine blue stain as described by Tanaka et al. (2004) (link). The stained areas were measured with ImageJ according to Cui et al. (2016) (link). Wounding-induced cell death was determined from leaves punctured with a needle. Wounded leaves at 6 days post wounding (dpw) were subjected to trypan blue staining to visualize the cell death. Samples were photographed with a stereomicroscope (Olympus SZX16, Japan) and measured with ImageJ. Each lesion was measured four times through its center. The mean of the four lengths of each wound was taken as the length of spread of cell death. For assessment of H2O2 production and Botrytis-induced cell death, spray-infected rosette leaves were stained with 3,3′-diaminobenzidine (DAB; D8001, Sigma-Aldrich) at 16 hours post infection (hpi) and trypan blue (T6146, Sigma) at 36 hpi. The stained leaves were mounted in water to eliminate reflection before being photographed with a stereomicroscope (Olympus SZX16, Japan). The DAB- and trypan blue-stained area and whole leaf area in each sample were measured with ImageJ. The percentage stained area was calculated by dividing the stained area by the whole leaf area.
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