Glomax multi e7031

The proliferation rate of MCF7 cells was determined using Cell Counting Kit-8 (CCK-8; Dojindo, Shanghai, China) according to the manufacturer’s instructions. Cells (5 × 10³ per well) were precultured in a tissue culture microplate with a clear bottom, followed by the addition of 10 μL of the CCK-8 solution to each well and incubation for 1 h at 37°C. The absorbance was measured at 450 nm using a microplate reader (GloMax^®-Multi E7031; Promega). All experiments were performed in triplicate.

Lactate production levels were determined using a lactate assay kit (DG-LAC200; DoGenBio, Korea). MDA-MB-231 cells (5 × 10⁴ per well) were plated in 24-well culture plates, and MCF7 cells (2 × 10⁴ per well) were seeded into the top chambers of a 24-well insert (BD Biosciences). After 24 h of incubation, the medium was completely removed and then each well with MCF7 cells was filled with fresh medium. The cells were incubated overnight in a single-culture state. The lactate assay was performed according to the manufacturer’s protocol using culture media collected from each sample. The absorbance was measured at 450 nm using a microplate reader (GloMax^®-Multi E7031; Promega).