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Mouse il 23 elisa kit

Manufactured by Thermo Fisher Scientific

The Mouse IL-23 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-23 (IL-23) in cell culture supernatants, serum, and plasma samples.

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2 protocols using mouse il 23 elisa kit

1

Cytokine Regulation of CD4+ T Cell

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CD4+CCR6+CXCR3- T cells were separated by cell sorting; CD11C+ APCs were separated using a CD11C positive selection kit (STEMCELL). Sorted CD4+CCR6+ CXCR3- T cells and CD11C+ APCs were co-cultured at a ratio of 2:1 with or without anti-GM-CSF (10 µg/mL; R&D Systems) or IL-38(10 ng/mL) for 24 hours. IL-23 levels in the supernatants were measured using mouse IL-23 ELISA kit (Thermo Fisher Scientific).
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2

ELISA Analysis of Cytokine Profiles

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After obtaining the PBMCs of healthy subjects and patients at different stages (initial onset, post‐treatment and recurrence due to SL), we conducted the following ELISA kit detection, obeying the manufacturer's instructions: human IL‐17A ELISA Kit (#88‐7176‐22), human IL‐23 ELISA Kit (#88‐7237‐22) and human GM‐CSF ELISA Kit (#88‐8337‐88). The supernatants from cell co‐culture medium were determined using the mouse IL‐23 ELISA Kit (#BMS6017). The serum from mice were determined using the mouse IL‐23 ELISA Kit (#BMS6017) and GM‐CSF mouse ELISA Kit (#BMS612) (Thermo Fisher Scientific).
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