L ornithine

Phosphate buffer saline solution (PBS) was made in-house and buffered to a pH of 7.3 using chemicals purchased from Fisher Scientific. NOHA was purchased from CALBIOCHEM. L-arginine was procured from Fisher Scientific. L-ornithine and L-citrulline were obtained from Sigma-Aldrich. All experiments, unless indicated, were performed using a Gamry 600+ Potentiostat and a BASi C3 Static Cell Faraday cage. Gamry software was used to record data, and the collected data was graphically displayed using Excel.
Rotating disk electrode (RDE) experiments were performed in a three-electrode assembly within a Pine Research Wavedriver RDE system containing a glassy carbon working electrode, platinum auxiliary electrode, and a Ag/AgCl reference electrode.
For fast scan cyclic voltammetry (FSCV) experiments, carbon fiber microelectrodes (CFE) were prepared by pulling an 11 μm carbon fiber into a glass capillary. The glass capillary was pulled to a fine tip in a pipette puller and sealed using epoxy resin (Epon 828 with a 14% m-phenlylenediamine by weight). The electrode was then polished to a fine tip using a diamond wheel polish at a 45° angle prior to use. More details can be found in published resources.^{22 ,23 (link)}