Mcf 7

Manufactured by Cobioer Biosciences

Sourced in China

The MCF-7 is a cell line derived from human breast adenocarcinoma. It is commonly used in laboratory research to study breast cancer biology and drug development.

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Lab products found in correlation

Mda mb 231, by Cobioer Biosciences (4 mentions) Bt 474, by Cobioer Biosciences (2 mentions) Mcf 10a, by Cobioer Biosciences (2 mentions) Ab6671, by Abcam (1 mentions) Mda mb 453, by Cobioer Biosciences (1 mentions) Mda mb 453, by MedChemExpress (1 mentions) Mda mb 231, by MedChemExpress (1 mentions) Gdc 0941, by MedChemExpress (1 mentions) Mda mb 468, by Cobioer Biosciences (1 mentions) Hepg2, by Cobioer Biosciences (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Mda mb 157, by Cobioer Biosciences (1 mentions) Si linc00461, by GenePharma (1 mentions) Mir 144 3p mimic, by GenePharma (1 mentions) Mir 144 3p inhibitor, by GenePharma (1 mentions) Lipofectamin 2000, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions)

7 protocols using mcf 7

Investigating Breast Cancer Cell Lines

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Breast cancer cell lines BT-474, MCF7, Hs-578-T, MDA-MB-231, MDA-MB-453, and MDA-MB-468 (Cobioer, Nanjing, China) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sunncell, Wuhan, China) with 10% fetal bovine serum (FBS) and certain cell growth factor. BT-549, HCC1937, and T47D cell lines were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Sunncell, Wuhan, China) with 10% or 20% FBS.
For the following function experiments, T47D, MCF7, MDA-MB-453, HCC1937, MDA-MB-231, and MDA-MB-468 cells were treated with 100 or 500 nM GDC-0941 (PI3K selective inhibitor, MedChemExpress, New Jersey, USA) or dimethyl sulfoxide (DMSO) for 24 h. In addition, MDA-MB-231 and HCC1937 cells were treated with 100 nM OSU-T315 (ILK inhibitor, SolelyBio, Beijing, China), tumor necrosis factor (TNF)-α, IgG, or TNF-α antibody (ab6671, Abcam, Cambridge, UK).
In addition, MCF7 and MDA-MB-453 cells were transfected with ILK, and MDA-MB-231 and HCC1937 cells were transfected with shILK.

Chen H., Cheng M., Gao P., Zhang X., Li G., Wang L., Qin L, & Li H. (2022). GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway. Bioengineered, 13(4), 10944-10955.

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Cell Cytotoxicity Assay Protocol

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All the cell lines used in this study were either purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (A549, HepG2, MCF-7, PC-3 and LO2) or from Nanjing Cobioer Biosciences Co., Ltd. (Karpas299). Cells were incubated with 5% CO₂ at 37 °C for 24 h. Subsequently the compounds and the reference were dissolved into the culture medium. Then, the cells were treated with different concentrations of test compounds and further incubated. After drug treatment, 20 μL of MTT solution at 5 mg mL⁻¹ was added and incubated for 4 h. 100 μL of DMSO was added into each well to dissolve the purple formazan formed. The absorbance was determined at 630 nm using a plate reader. The IC₅₀ was calculated using GraphPad Prism version 6.0 software (San Diego, USA) from the non-linear curve.

Cai D., Zhang Z.H., Chen Y., Ruan C., Li S.Q., Chen S.Q, & Chen L.S. (2020). Design, synthesis and biological evaluation of novel amide-linked 18β-glycyrrhetinic acid derivatives as novel ALK inhibitors. RSC Advances, 10(20), 11694-11706.

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Paclitaxel-Resistant MCF-7 Cell Line

Cited 1 time

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The human BC cell line MCF-7 was obtained from COBIOER (Nanjing, China) and incubated in a paclitaxel for over 6 months, and we gradually increased the paclitaxel concentration[4 (link)]. Thereafter, MCF-7/PR cell line was established. The DMEM medium (Gibco, Waltham, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) containing 100 mg/mL streptomycin (Sigma-Aldrich, St-Louis, MO, USA) and 100 U/mL penicillin (Sigma-Aldrich) was used to incubate cells. Cell incubation was under a humid condition containing 5% CO₂ at 37°C.

Chen Y., Li X., Xu J., Xiao H., Tang C., Liang W., Zhu X., Fang Y., Wang H, & Shi J. (2022). Knockdown of nuclear receptor binding SET domain-containing protein 1 (NSD1) inhibits proliferation and facilitates apoptosis in paclitaxel-resistant breast cancer cells via inactivating the Wnt/β-catenin signaling pathway. Bioengineered, 13(2), 3526-3536.

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Culturing MCF-7 Breast Cancer Cells

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The human breast cancer cell line MCF-7 was purchased from Cobioer Biosciences Corporation (Nanjing, China) and cultured in DMEM containing 10% FBS at 37 °C in a humidified atmosphere of 5% CO₂ and 95% air.

Wang B., Xi C., Liu M., Sun H., Liu S., Song L, & Kang H. (2018). Breast fibroblasts in both cancer and normal tissues induce phenotypic transformation of breast cancer stem cells: a preliminary study. PeerJ, 6, e4805.

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Breast Cell Line Transfection Protocol

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Human normal breast epithelial cell line MCF 10A (CBP60419) and breast cancer cell lines AU565 (CBP60353), MCF-7 (CBP60380), MDA-MB-157 (CBP60381) and MDA-MB-231 (CBP60382) were all purchased from Cobioer (Nanjing, China). Plasmids including si-NC, si-LINC00461, inhibitor NC, miR-144-3p inhibitor, mimic NC, miR-144-3p mimic, oe-NC, oe-KPNA2 were ordered from GenePharma (Shanghai, China), and the siRNAs were sequenced as below: si-LINC00461: 5′-CTGCAAAGAAGCATAAAATGA-3′; si-NC: 5′-TTCTCCGAACGTG TCACGT-3′.
Before transfection, cells were grown in 6-well plates (3 × 10⁵ cells/well) until the density reached 50%. 250 μL of serum-free Opti-MEM (51985042, Gibco, Gaitherburg, MD, USA) was used to dilute the target plasmids (4 μg) and Lipofectamin 2000 reagent (10 μL; 11668-019, Invitrogen, NY, California, USA), respectively. Thereafter, the dilutions were mixed, and then dripped into cells after 20 min. All cells were maintained in 5% CO₂ at 37 °C for 6 h, and cultured for additional 36–48 h with fresh mediums.

Zhang Q., Jin X., Shi W., Chen X., Pang W., Yu X, & Yang L. (2020). A long non-coding RNA LINC00461-dependent mechanism underlying breast cancer invasion and migration via the miR-144-3p/KPNA2 axis. Cancer Cell International, 20, 137.

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Culturing Normal and Breast Cancer Cells

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Normal MCF‐10A cells and BC cell lines (MCF‐7, MDA‐MB‐231, BT‐549, and T47D) were purchased from COBIOER. BC cell lines were cultured in the RPMI‐1640 medium (COBIOER) plus 1% penicillin–streptomycin (COBIOER) and 10% fetal bovine serum (FBS, COBIOER). MCF‐10A cells were grown in MEBM BulletKit. All medium was maintained at 37°Cwith 5% CO₂.

Liu Y., Liu Y., Luo J., Zhao W., Hu C, & Chen G. (2022). Hsa_circ_0002082 up‐regulates Centromere Protein F via abolishing miR‐508‐3p to promote breast cancer progression. Journal of Clinical Laboratory Analysis, 36(11), e24697.

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Culturing Breast Cancer Cell Lines

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Human TNBC line MDA-MB-231, luminal B type of breast cancer cell-line BT474, luminal A type of breast cancer cell-line T47D and MCF-7, and MCF-10A cells (human mammary epithelial cells) were purchased from COBIOER (Nanjing, China). L-15 medium (Thermo Fisher Scientific, Waltham, MA, USA) and DMEM medium with 10% FBS (fetal bovine serum, Thermo Fisher) were used to culture MDA-MB-231 and other cell lines at 37°C under a circumstance containing 5% CO₂, respectively.

Yang B., Wang F, & Zheng G. (2021). Transmembrane protein TMEM119 facilitates the stemness of breast cancer cells by activating Wnt/β-catenin pathway. Bioengineered, 12(1), 4856-4867.

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