Celltrace oregon green 488

Manufactured by Thermo Fisher Scientific

CellTrace Oregon Green 488 is a fluorescent dye used for cell proliferation and cell tracking studies. It provides bright and stable fluorescence that can be detected using standard green fluorescence detection methods.

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Lab products found in correlation

Lsrfortessa x 20, by BD (1 mentions) Celltrace far red, by Thermo Fisher Scientific (1 mentions) Anti cd64 10, by BioLegend (1 mentions) Tcs sp8, by Leica (1 mentions) Celltrace far red cell proliferation kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Oregon Green 488 (75 citations) Oregon Green (29 citations) CellTrace Oregon Green (5 citations) CellTrace Oregon Green 488 Carboxy-DFFDA (2 citations)

2 protocols using celltrace oregon green 488

Tumor-Macrophage Interaction Assay

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Tumor cells were labeled with CellTrace Oregon Green 488 (Invitrogen) and incubated with antibodies at 37°C in 5% CO₂ for 15 minutes. Then, human monocyte-derived macrophages (MDM) labeled with CellTrace Far Red (FR; Invitrogen) were added to tumor cells (1:2 E: T) and incubated at 37°C in 5% CO₂ for 3 hours before analysis. For some experiments, macrophages were incubated with 10 μg/mL anti-CD16 (3G8, BioLegend), anti-CD32 (FLI8.26, STEMCELL), and anti-CD64 (10.1, BioLegend) for 15 minutes to neutralize Fc receptors prior to tumor coculture. Data were collected on a flow cytometer (BD LSR Fortessa X-20) and analyzed using FlowJo software.

Cao A., Yi J., Tang X., Szeto C.W., Wu R., Wan B., Fang X., Li S., Wang L., Wang L., Li J., Ye Q., Huang T., Hsu K., Kabbarah O, & Zhou H. (2022). CD47-blocking Antibody ZL-1201 Promotes Tumor-associated Macrophage Phagocytic Activity and Enhances the Efficacy of the Therapeutic Antibodies and Chemotherapy. Cancer Research Communications, 2(11), 1404-1417.

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Quantifying Angiogenesis in 3D Spheroids

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Before spheroid formation, ECs were stained with CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) and MSCs were stained with CellTrace™ Far Red Cell Proliferation Kit (both from Invitrogen). Confocal microscopy was performed using the Leica TCS SP8 (Leica, Wetzlar, Germany). Images were acquired as 2D slices and processed and analyzed in ImageJ (NIH, Bethesda, MD). For analysis of images, confocal images were converted to 500 × 500 pixel size, converted to binary, and quantified using the Angiogenesis Analyzer plugin. Segments were defined as lines connected to other components on both ends. Branches were defined as lines that are connected to a segment on one end and free on the other. Meshes were defined as closed loops of segments. Total length was defined as the total of segments, branches and isolated elements in the analyzed area [24 ]. Values are reported by cell population channel acquired for each spheroid group.

Vorwald C.E., Joshee S, & Leach J.K. (2020). Spatial localization of endothelial cells in heterotypic spheroids influences NOTCH signaling. Journal of molecular medicine (Berlin, Germany), 98(3), 425-435.

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