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Scc 4

Manufactured by American Type Culture Collection
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The SCC-4 is a piece of laboratory equipment designed for cell culture applications. It provides temperature and CO2 control to maintain optimal conditions for cell growth and proliferation. The core function of the SCC-4 is to regulate the temperature and CO2 levels within a controlled environment to support the culturing of cells.

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Lab products found in correlation

Cal27, by American Type Culture Collection (26 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (22 mentions) Scc25, by American Type Culture Collection (17 mentions) Scc15, by American Type Culture Collection (11 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (10 mentions) Hydrocortisone, by Merck Group (9 mentions) Dmem f12, by Thermo Fisher Scientific (7 mentions) Scc 4, by Thermo Fisher Scientific (6 mentions) Penicillin, by Thermo Fisher Scientific (5 mentions) Streptomycin, by Thermo Fisher Scientific (5 mentions) Hsc 3, by Sekisui (4 mentions) Hsc 2, by American Type Culture Collection (3 mentions) Skn 3, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Schneider s drosophila medium, by Thermo Fisher Scientific (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Mda 231, by American Type Culture Collection (2 mentions) Skn 3, by Thermo Fisher Scientific (2 mentions) Nonessential amino acid, by Corning (2 mentions) Accutase, by Innovative Cell Technologies (2 mentions)

Close spelling variants of this product

SCC-4 cell line (2 citations)

43 protocols using scc 4

1

Establishment and Authentication of HNSCC Cell Lines

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Human HNSCC cell lines FaDu, Cal27, SCC4, SCC9 and SCC25 were purchased from ATCC (https://www.lgcstandards-atcc.org). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% foetal bovine serum (Invitrogen, Germany), 2 mM l-glutamine and 50 μg/ml penicillin-streptomycin in humidified and sterile conditions with 6% CO2 at 37 °C. Cell cultures were regularly screened to exclude mycoplasma contamination (Venor®GeM Classic Mycoplasma Detection Kit, Minerva Biolabs) according to manufacturer’s recommendation, and the authentication of all cell lines was confirmed by the Multiplex Human Cell Line Authentication Test (Multiplexion, Germany, latest update April 2019).
Rong C., Muller M.F., Xiang F., Jensen A., Weichert W., Major G., Plinkert P.K., Hess J, & Affolter A. (2020). Adaptive ERK signalling activation in response to therapy and in silico prognostic evaluation of EGFR-MAPK in HNSCC. British Journal of Cancer, 123(2), 288-297.
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2

Cell Line Maintenance and Identification

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CAL-27, SCC-4, SCC-9 and SCC-25 cell lines were purchased from the ATCC and maintained according to the ATCC recommendations. WSU-HN-4, HN-6, HN-13 cell lines and normal epithelial cells (NECs) were acquired and cultured as well as genetic identified as described in the previous study [18 (link), 19 (link)].
Cao W., Liu J., Xia R., Lin L., Wang X., Xiao M., Zhang C., Li J., Ji T, & Chen W. (2016). X-linked FHL1 as a novel therapeutic target for head and neck squamous cell carcinoma. Oncotarget, 7(12), 14537-14550.
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3

Oral Cancer Cell Lines RNA Extraction

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The oral cancer cell lines Cal27, SCC-4, SCC-9, and SCC-25 were obtained from ATCC and the oral dysplastic line DOK was obtained from the European Collection of Cell Cultures. Cell lines were maintained according to distributor recommendations. 293T and HMEC1 cells were received as a gift from Dr. Aly Karson and were cultured in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum (FBS) at 37°C. RNA was extracted from cells and SEVs using the miRCURY RNA Isolation Kit (Exiqon) per manufacturer instructions.
Dickman C.T., Lawson J., Jabalee J., MacLellan S.A., LePard N.E., Bennewith K.L, & Garnis C. (2017). Selective extracellular vesicle exclusion of miR-142-3p by oral cancer cells promotes both internal and extracellular malignant phenotypes. Oncotarget, 8(9), 15252-15266.
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4

Cell Line Characterization and Cultivation Protocol

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Cal-27, SCC-4, SCC-9, HeLa, H460, MDA-231, and PC-3 cells were obtained from ATCC (Manassas, VA, USA). HSC-3 cells were provided by Dr. Brian Schmidt at New York University College of Dentistry. Cell lines were authenticated by Genetica DNA Laboratories (Cincinnati, OH, USA) and maintained in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin and incubated at 37°C in 5% CO2. OKF6-TERT-2 cells were obtained from Harvard Medical School Cell Culture Core Collection, Cambridge, MA and cultured as previously reported (10 (link)). CHO cells overexpressing TRPV1 (CHO-TRPV1) were provided by Dr. Ardem Patapoutian at the Scripps Research Institute and cultured as described (11 (link)).
De La Chapa J.J., Singha P.K., Self K.K., Sallaway M.L., McHardy S.F., Hart M., McGuff H.S., Valdez M.C., Ruiz F I.I., Polusani S.R, & Gonzales C.B. (2019). The Novel Capsazepine Analog, CIDD-99, Significantly Inhibits Oral Squamous Cell Carcinoma In Vivo Through a TRPV1-Independent Induction of ER Stress, Mitochondrial Dysfunction, and Apoptosis. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48(5), 389-399.
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5

Cell Culture Protocol for Oral Squamous Carcinoma

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All TSCC cell lines, including Cal 27, SCC-9, SCC-25 and SCC-4 were purchased from the ATCC (Manassas, VA, USA). SCCO22 was derived from our previous publication[58 (link)]. All cells were grown in DMEM (Thermo Fisher Scientific, Waltham, MA, USA, #11965118) medium containing 10% fetal bovine serum (Biological Industries, Kibbutz Beit Haemek, Israel, #1809249),100 IU/ml penicillin and 100 mg/ml streptomycin (Thermo Fisher Scientific, #1935444).
Oral human keratinocytes (OHKCs) were isolated from tongue tissues according to a previously published protocol for isolation of keratinocytes from skin tissues[59 (link)]. The OHKCs were cultured in keratinocyte serum free medium (K-SFM, Thermo Fisher Scientific, #10725018) in culture dishes pretreated with a coating matrix containing type-I collagen (Gibco, R-011-K) and the medium was changed every 2 days.
Xu L., Zu T., Li T., Li M., Mi J., Bai F., Liu G., Wen J., Li H., Brakebusch C., Wang X, & Wu X. (2021). ATF3 downmodulates its new targets IFI6 and IFI27 to suppress the growth and migration of tongue squamous cell carcinoma cells. PLoS Genetics, 17(2), e1009283.
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6

Cell Culture and Viability Assay Protocol

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The cell lines used in the present study were: HGF—human primary gingival fibroblasts (ATCC® PCS-201-018™), SCC-4—human squamous cell carcinoma cell line (ATCC® CRL-1624™), HaCaT—immortalized human keratinocytes (CLS Cell Lines Service GmbH), and A375—human melanoma cells (ATCC® CRL-1619™).
For cell culture and cell viability assay, the following reagents were needed: specific culture medium—Fibroblast Basal Medium (ATCC PCS-201-030) and Fibroblast growth kit—low serum (ATCC PCS-201-041) for HGF cells, DMEM:F12 Medium (ATCC® 30-2006™)—for SCC-4 cells were acquired from ATCC, and Dulbecco’s Modified Eagle Medium (DMEM) high glucose −4.5 g/L for HaCaT and A375 cells, together with the other reagents used, as: trypsin—EDTA solution, PBS (phosphate saline buffer), fetal bovine serum (FCS), Trypan blue, Alamar blue (resazurin sodium salt) and hydrocortisone were purchased from Sigma Aldrich (Darmstadt, Germany) and Thermo Fisher Scientific (Waltham, MA, USA).
Alexa V.T., Galuscan A., Soica C.M., Cozma A., Coricovac D., Borcan F., Popescu I., Mioc A., Szuhanek C., Dehelean C.A, & Jumanca D. (2022). In Vitro Assessment of the Cytotoxic and Antiproliferative Profile of Natural Preparations Containing Bergamot, Orange and Clove Essential Oils. Molecules, 27(3), 990.
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7

Cell Lines Used for Oral Squamous Cell Carcinoma

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SCC4, SCC15, SCC90, and SCC152 cells were from ATCC (Manassas, VA USA). UMSCC47 (SCC47) was from Sigma‐Aldrich (St. Louis, MO USA). OCTT2 cell line was derived from a surgical specimen of an oral SCC tumor [30 (link)]. VU147T was from Dr. Hans Joenje, VU Medical Center, the Netherlands. All cancer cell lines were grown in submersion in high glucose Dulbecco's modified Eagle medium (Gibco; Gaithersburg, MD, USA) plus 10% FBS, penicillin/streptomycin mix (Gibco), and nonessential amino acids (Gibco). Primary gingival keratinocytes were from ATCC (Manassas, VA, USA) and used within 2 passages using dermal cell basal keratinocyte medium (ATCC).
Carey R.M., McMahon D.B., Miller Z.A., Kim T., Rajasekaran K., Gopallawa I., Newman J.G., Basu D., Nead K.T., White E.A, & Lee R.J. (2021). T2R bitter taste receptors regulate apoptosis and may be associated with survival in head and neck squamous cell carcinoma. Molecular Oncology, 16(7), 1474-1492.
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8

Culturing Human Gingival Fibroblasts and Squamous Cell Carcinoma

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The following cell lines were used in this study: human gingival fibroblasts—HGF (PCS-201-018™) and squamous cell carcinoma cell line—SCC-4 (CRL-1624™), both purchased from ATCC. The acquired cells came as frozen vials and were kept in liquid nitrogen. The cell lines were cultured in their specific growth medium: HGF in Fibroblast Basal Medium which was completed with the Fibroblast Growth Kit-Low Serum; 10% FCS and 1% antibiotic mixture penicillin–streptomycin–amphotericin B Solution, 0.5 mL (Final concentration penicillin: 10 Units/mL, streptomycin: 10 µg/mL, and amphotericin B: 25 ng/mL); and SCC-4 in DMEM: F12 Medium supplemented with 400 ng/mL hydrocortisone 21-hemisuccinate sodium salt, 10% FCS, and 1% penicillin (100 U/mL)–streptomycin (100 μg/mL). The cells were cultivated under standard culture conditions at 37 °C and 5% CO2 in a humidified atmosphere.
Surducan D.A., Racea R.C., Cabuta M., Olariu I., Macasoi I., Rusu L.C., Chiriac S.D., Chioran D., Dinu S, & Pricop M.O. (2022). Eugenol Induces Apoptosis in Tongue Squamous Carcinoma Cells by Mediating the Expression of Bcl-2 Family. Life, 13(1), 22.
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9

Culturing Oral Cancer Cell Lines

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All cell lines were cultured in 10 cm diameter cell culture dishes at 37°C with 5% CO2. Human oral squamous cell carcinoma (SCC) cell line, HSC3 (Sekisui XenoTech, Kansas City, KS), non-tumorigenic cell line HaCaT (ThermoFisher), were cultured in DMEM (Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (FBS; Corning) and penicillin/streptomycin (P/S, 50 U/mL, Corning; DMEM-COMP). SCC cell line SCC-4 (ATCC, Manassas, VA) was cultured in DMEM/F12 (Gibco) supplemented with 10% FBS and P/S. Dysplastic oral keratinocyte cell line DOK (Sigma-Aldrich, St. Louis, MO) was cultured in DMEM supplemented with 10% FBS, P/S, and 5μg/mL hydrocortisone (Sigma-Aldrich). Human cell line authenticity was independently verified by PCR using short tandem repeat profiles (ATCC). Mouse oral SCC cell lines MOC1 and MOC2 (Kerafast) were cultured in IMDM/F12 (2:1; Gibco) supplemented with 5% FBS, P/S, 5 μg/mL insulin (Sigma-Aldrich), 40 ng/mL hydrocortisone, and 5 ng/mL epidermal growth factor (EMD Millipore; MOC-COMP) Cell pellets for gene expression analysis from all cell lines were collected from a passage number less than 14.
McIlvried L.A., Atherton M.A., Horan N.L., Goch T.N, & Scheff N.N. (2022). Sensory neurotransmitter calcitonin gene-related peptide modulates tumor growth and lymphocyte infiltration in oral squamous cell carcinoma. Advanced biology, 6(9), e2200019.
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10

Cell Culture Protocol for Oral Cancer Cell Lines

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HSC-2, CAL-27 and SCC-4 cell lines were purchased from ATCC and were cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) containing with 10% fetal bovine serum at 37°C in a 5% CO2 incubator. The normal human oral keratinocyte (NHOK) cells which served as control cells were obtained from the ScienCell Research Laboratories, Inc. (cat. no. 2610) and cultured as previously described (15 (link)).
He L., Liao L, & Du L. (2020). miR-144-3p inhibits tumor cell growth and invasion in oral squamous cell carcinoma through the downregulation of the oncogenic gene, EZH2. International Journal of Molecular Medicine, 46(2), 828-838.
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