Hematoxylin eosin

Gross specimen observations, histological observations, and behavioral evaluations were performed at 1, 3, 6, and 12 weeks after transplantation. The evaluation criteria for neurological function were based on the Combined Behavioral Score Scale (Kerasidis et al., 1987). Evaluation categories included movement in open space, toe stretching, ground touching, reflex, posture change, ramp climbing, and swimming. The maximum score is 100 points for normal activity and 0 points for complete paralysis of the hind limbs.
Histological changes in the spinal cord after SCI were evaluated by hematoxylin-eosin staining (Beyotime, Shanghai, China). Stained sections can show the repair of tissue structure and the regeneration of blood vessels and neurons. The histology of the spinal cord was also examined by Pischingert’s methylene blue staining (Beyotime, Shanghai, China). Nissl bodies were dyed blue with methylene blue, which can reveal neuronal regeneration. The neuronal results were observed with an optical microscope (CX23; Olympus).

The freshly removed tissue from the Control, NC, and IDO groups, as well as the tumor tissue from the HepG2 group, was fixed in 4% paraformaldehyde for 24 h, dehydrated, paraffin-embedded, sectioned, and stained with Hematoxylin and eosin according to the protocol described previously [22 (link)]. The acquired paraffin slices were deparaffinized, stained with hematoxylin-eosin (Cat #C0105 M, Beyotime), dried, and then mounted. Using an optical microscope, histological changes were found in the tissue sections (Nikon DS-U3).