Bdh aristar ultra concentrated nitric acid

Dulbecco’s modified Eagle’s medium (DMEM), GlutaMAX, sodium pyruvate and non-essential amino acids were obtained from Life Technologies. Fatty acid-free, ultra-pure BSA and protease inhibitor cocktail were obtained from Roche. BCS, copper chloride, 8-bromo-cAMP, dibutyryl cAMP, IBMX, isoproterenol, and free glycerol reagent were obtained from Sigma-Aldrich. BDH Aristar Ultra concentrated nitric acid was obtained from VWR. NEFA kits were obtained from Wako. H-89 dihydrochloride, cilostamide, and rolipram were obtained from EMD Biosciences. CAY10499 and cyclic AMP assay kits were obtained from Cayman Chemical. Restriction endonucleases, Phusion High-Fidelity DNA polymerase, T4 DNA ligase, and Gibson Assembly Master Mix were obtained from New England Biolabs. TaKaRa ExTaq DNA polymerase was purchased from Clontech. Primers were purchased from Integrated DNA Technologies. All other materials used in biochemical assays were purchased from Sigma unless otherwise noted. Baculoviral particles were generated using the BaculoGold™ transfection kit from BD Biosciences. All DNA constructs were verified by sequencing by Quintara Biosciences, and all plasmids used for transfections were prepared using the EndoFree Maxi Kit (Qiagen).