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B6 thy1

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B6 Thy1.1 is a purified monoclonal antibody produced in mouse. It is designed for use as an immunoglobulin isotype control in flow cytometry and other immunoassays.

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Lab products found in correlation

C57bl 6, by Jackson ImmunoResearch (4 mentions) B6 ly5, by Jackson ImmunoResearch (3 mentions) B6 rag1 mice, by Jackson ImmunoResearch (2 mentions) B6 il27ra mice, by Amgen (2 mentions) B6 nr4a1egfp nur77gfp, by Jackson ImmunoResearch (2 mentions) B6 mhc 2, by Jackson ImmunoResearch (2 mentions) B6 rag1, by Jackson ImmunoResearch (1 mentions) Cd11ccre gfp, by Jackson ImmunoResearch (1 mentions) Cd4ko, by Jackson ImmunoResearch (1 mentions) Rosa26lsl yfp, by Jackson ImmunoResearch (1 mentions) Rorcgfp gfp, by Jackson ImmunoResearch (1 mentions) Rorc cre, by Jackson ImmunoResearch (1 mentions) Cd4 cre, by Jackson ImmunoResearch (1 mentions) B6 cd45.1 mice, by Charles River Laboratories (1 mentions) C57bl 6j b6, by Jackson ImmunoResearch (1 mentions) B6 cd45, by Jackson ImmunoResearch (1 mentions)

8 protocols using b6 thy1

1

Partial Bone Marrow Ablation for Chimera

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Bone marrow (BM) chimera was established as previously described19 (link). For BM transplantation, BM recipient male mice (6–8 weeks old) were injected intraperitoneally with busulfan. The dose included a 1:1 solution of DMSO and deionized water (30 mg/kg/100 μl) once daily for 2 days resulting in partial ablation of the bone marrow19 (link). Alternative approach of BM ablation using whole body irradiation, results in systemic toxicities. Moreover, irradiation resulted in inflammation of skin. To circumvent these issues, a widely used myelo-supressive approach for partial bone marrow ablation using busulfan was selected. Forty  eight  hours after the last dose of busulfan, donor BM cells isolated from femur were injected to recipient mice via tail vein injection (100 μl) to recipient mice. Engraftment in recipient mice was allowed to take place over a duration of 4 weeks after which engraftment was ascertained by determining the presence of GFP cells in the BM and the blood. The study utilized two types of recipient mice: (i) C57BL/6 obtained from Envigo laboratories and (ii) B6 Thy1.1 (Jackson# 000406) with CD90.1 allele.
Sinha M., Sen C.K., Singh K., Das A., Ghatak S., Rhea B., Blackstone B., Powell H.M., Khanna S, & Roy S. (2018). Direct conversion of injury-site myeloid cells to fibroblast-like cells of granulation tissue. Nature Communications, 9, 936.
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2

Mouse Strains for Immunology Research

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C57BL/6, B6 Ly5.1, B6 Thy1.1, B6 Rag1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). B6 Il27ra−/− mice were obtained from Amgen (Thousand Oaks, CA). B6 Foxp3GFP 41 (link) and B6 Lag3−/− mice were obtained from Drs. Yasmine Belkaid and Christophe Benoist, respectively. All animal procedures were conducted according to the guidelines of the Institutional Animal Care and Use Committee.
Do J.S., Visperas A., Sanogo Y.O., Bechtel J.J., Dvorina N., Kim S., Jang E., Stohlman S.A., Shen B., Fairchild R.L., Baldwin WM I.I.I., Vignali D.A, & Min B. (2015). An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell suppressive function and therapeutic efficacy. Mucosal immunology, 9(1), 137-145.
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3

Mouse Strains for Immunology Research

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C57BL/6, B6 Ly5.1, B6 Thy1.1, B6 Rag1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). B6 Il27ra−/− mice were obtained from Amgen (Thousand Oaks, CA). B6 Foxp3GFP 41 (link) and B6 Lag3−/− mice were obtained from Drs. Yasmine Belkaid and Christophe Benoist, respectively. All animal procedures were conducted according to the guidelines of the Institutional Animal Care and Use Committee.
Do J.S., Visperas A., Sanogo Y.O., Bechtel J.J., Dvorina N., Kim S., Jang E., Stohlman S.A., Shen B., Fairchild R.L., Baldwin WM I.I.I., Vignali D.A, & Min B. (2015). An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell suppressive function and therapeutic efficacy. Mucosal immunology, 9(1), 137-145.
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4

Mouse Strains for Immunological Studies

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C57BL/6, B6 Ly5.1, B6 Thy1.1, B6 IFNγR−/−, B6 Rag1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). IFNγR−/− Rag−/− mice were bred at the animal facility of the Lerner Research Institute. All animal procedures were conducted according to the guidelines of the Institutional Animal Care and Use Committee.
Do J.S., Asosingh K., Baldwin WM I.I.I, & Min B. (2014). IFNγR signaling in non-T cell targets regulates T cell-mediated intestinal inflammation through multiple mechanisms. Journal of immunology (Baltimore, Md. : 1950), 192(6), 2537-2541.
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5

Diverse Murine Strains for Immunology

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C57Bl/6 (B6), B6.CD45.1, B6.Thy1.1, B6.Nr4a1eGFP (Nur77GFP), B6.CD80/CD86 KO, IL-15Ra KO, CD11c-Cre-GFP, I-AB-flox, CD4 KO and B6.MHC II− were obtained from the Jackson Laboratory. B6.TCRα/β KO mice were obtained from R.Welsh (UMass Chan), Y-linked B6.OT-II mice from L.Bradley (The Scripps Research Institute, La Jolla, CA) and were originally published by F.Carbone’s group 47 (link), BALB/c.HNT from D. Lo 48 (link) (The Scripps Research Institute, La Jolla, CA), IFNAR KO from J.Sprent (Garvan Institute), CD11cTg.H2-Ab1−/− mice from T.Laufer originally and all have been bred and maintained at the UMass Chan animal facility. Mice were at least 8 weeks old prior to use, controls were age and sex-matched, both female and male mice were used where possible. Experimental animal procedures were done in accordance with UMass Chan Animal Care and Use Committee guidelines.
Bussey H., Kaback D.B., Zhong W., Vo D.T., Clark M.W., Fortin N., Hall J., Ouellette B.F., Keng T, & Barton A.B. (1995). The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.. Proceedings of the National Academy of Sciences of the United States of America, 92(9), 3809-3813.
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6

Generation and Characterization of Conditional Knockout Mice

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Rag1−/−, Rorcgfp/gfp, Rorc-cre, Cd4-cre, Rosa26LSL-YFP/+, and B6-Thy1.1 mice were purchased from Jackson laboratory. Rag2−/−Il2rg−/− mouse was purchased from Taconic Biosciences. Smarca4flox/flox(Smarca4f/f) mouse was generated previously.43 (link)Rag1−/−Smarca4f/f mice were crossed to Rag1−/−Smarca4f/fRorc-cre mice to generate littermate Rag1−/−Smarca4f/f and Rag1−/−Smarca4ΔILC3 mice, which were used for experiments in this study. Littermate Rag1−/−Smarca4f/f and Rag1−/−Smarca4ΔILC3 mice were kept co-housed after weaning and during experiments. And for cases in which Rag1−/−Smarca4f/f and Rag1−/−Smarca4ΔILC3 from different litters were used for experiments, they were gender/age matched and co-housed for more than 2 weeks. For mice of other genotypes used in this study, Brg1-deficient mice and control mice were littermate controlled and co-housed. Both male and female mice were used in this study. All mice were on C57BL/6 background and maintained in specific pathogen free facilities at Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences. All mice experiments were performed in compliance with the guide for the care and use of laboratory animals, approved by the institutional biomedical research ethics committee of the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
Qi X., Qiu J., Chang J., Ji Y., Yang Q., Cui G., Sun L., Chai Q., Qin J, & Qiu J. (2020). Brg1 restrains the pro-inflammatory properties of ILC3s and modulates intestinal immunity. Mucosal Immunology, 14(1), 38-52.
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7

Mouse Strain Characterization for Immunology

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C57BL/6J (B6), C57BL/6-Tg (UBC-GFP)30Scha/J (ubiquitin-GFP), Tg(CAG-ECFP)CK6Nagy/J (actin-ECFP), B6.129S2(C)-Itgaetm1Cmp/J (CD103KO), B6.Cg-Tg(Itgax-Venus)1Mnz/J, and B6 Thy1.1 mice were purchased from The Jackson Laboratory. B6 CD45.1 mice were purchased from Charles River Laboratories. All mice were maintained in specific pathogen free conditions at the University of Minnesota. iFABP-OVA, P14/CD45.1, and OT-I/CD45.1 mice were bred and maintained in house. Kaede mice were a generous gift from Dr. Kristin Hogquist (University of Minnesota). P14 and OT-I reporter mice were generated by crossing the TCR Tg mice with ubiquitin-GFP or actin-ECFP mice. P14-CFP+ CD103−/− mice were generated by crossing P14-CFP+ mice to P14+ CD103−/− mice. Kaede B6 mice were crossed to OT-I mice to generate OT-I/Kaede reporter mice (Tomura et al., 2008 ). Adult male and female adult mice ranging from 10–32 weeks of age were used in experiments. All mice were housed under standard conditions in animal facilities and used in accordance with the Institutional Animal Care and Use Committees guidelines at the University of Minnesota.
Thompson E.A., Mitchell J.S., Beura L.K., Torres D., Mrass P., Pierson M.J., Cannon J.L., Masopust D., Fife B.T, & Vezys V. (2019). Interstitial migration of CD8αβ T cells in the small intestine is dynamic and is dictated by environmental cues. Cell reports, 26(11), 2859-2867.e4.
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8

Adoptive Transfer of T Cells

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C57BL/6J (B6), B6.CD45.1, B6.Thy1.1, B6.Nr4a1eGFP (Nur77GFP), and B6.MHC-II− were obtained from the Jackson Laboratory. Y-linked B6.OT-II mice were obtained from Dr. Linda Bradley and were originally published by Dr. Frank Carbone’s group (49 (link)); JhD mice were obtained from Dr. Mark Shlomchik; CD11cTg.H2-Ab1−/− mice were obtained from Dr. Terri Laufer; BALB/c.HNT were obtained from Dr. David Lo (50 (link)), and these strains were bred and maintained at the UMMS animal facility. Mice were at least 8 wk old prior to use.
Devarajan P., Vong A.M., Castonguay C.H., Kugler-Umana O., Bautista B.L., Jones M.C., Kelly K.A., Xia J, & Swain S.L. (2022). Strong influenza-induced TFH generation requires CD4 effectors to recognize antigen locally and receive signals from continuing infection. Proceedings of the National Academy of Sciences of the United States of America, 119(8), e2111064119.
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