Each of the OMP preparations (5 μg) were run on the Novex Bis-Tris pre-cast gel system with MOPS running buffer according to the manufacturer's instructions (Life Technologies). Ammoniacal silver staining was carried out to visualize proteins. Western blotting was carried out using nitro-cellulose membranes (Bio-Rad) and standard protocols68 . All mouse (AD6 anti-HMW1/2A34 (link); 1F4 anti-Hia35 (link)) and rabbit (LB1 anti-OMP P5 (ref. 28 (link)); chimV4 anti-OMP P5; anti-OMP P5) primary antibodies were used at a dilution of 1:2,500; all chinchilla (anti-OMP P2; anti-LPD27 (link); anti-PDM27 (link); anti-OMP P5/P6) primary antibodies at a dilution of 1:250. Anti-mouse-AP and anti-rabbit-AP secondary antibodies were used at a dilution of 1:5,000 (Sigma-Aldrich); protein A-AP secondary antibody was used at a dilution of 1:500 (Sigma-Aldrich) in blots where chinchilla primary antibodies were used. Blots were developed using SigmaFAST NBT/BCIP tablets according to the manufacturer's instructions (Sigma-Aldrich). All the primary antibodies were raised by the authors laboratories, with specific references for those described previously.
Protein a ap secondary antibody
Manufactured by Merck Group
Protein A-AP secondary antibody is a laboratory reagent used in various immunoassays and immunochemical techniques. It is composed of Protein A, a bacterial protein that binds to the Fc region of immunoglobulins, coupled to alkaline phosphatase (AP), an enzyme that catalyzes a color-producing reaction. This secondary antibody can be used to detect and quantify the presence of primary antibodies in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose membrane, by Bio-Rad (2 mentions)
Mops running buffer, by Thermo Fisher Scientific (2 mentions)
Novex bis tris pre cast gel system, by Thermo Fisher Scientific (2 mentions)
Anti mouse ap and anti rabbit ap secondary antibodies, by Merck Group (2 mentions)
Sigmafast nbt bcip tablets, by Merck Group (2 mentions)
2 protocols using protein a ap secondary antibody
1
Protein Expression Analysis by Western Blot
2
Protein Visualization and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Each of the OMP preparations (5 μg) were run on the Novex Bis-Tris pre-cast gel system with MOPS running buffer according to the manufacturer's instructions (Life Technologies). Ammoniacal silver staining was carried out to visualize proteins. Western blotting was carried out using nitrocellulose membranes (Bio-Rad) and standard protocols68 . All mouse (AD6 anti-HMW1/2A34 (link); 1F4 anti-Hia35 (link)) and rabbit (LB1 anti-OMP P5 (ref. 28 (link)); chimV4 anti-OMP P5; anti-OMP P5) primary antibodies were used at a dilution of 1:2,500; all chinchilla (anti-OMP P2; anti-LPD27 (link); anti-PDM27 (link); anti-OMP P5/P6) primary antibodies at a dilution of 1:250. Anti-mouse-AP and anti-rabbit-AP secondary antibodies were used at a dilution of 1:5,000 (Sigma-Aldrich); protein A-AP secondary antibody was used at a dilution of 1:500 (Sigma-Aldrich) in blots where chinchilla primary antibodies were used. Blots were developed using SigmaFAST NBT/BCIP tablets according to the manufacturer's instructions (Sigma-Aldrich). All the primary antibodies were raised by the authors laboratories, with specific references for those described previously.
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