hDFCs were seeded in a 24-well plate at a density of 1 × 104 cells/well in GM and were cultured in GM for 4 days until 80% confluent. A scratch was then made on the monolayer using a Cell Scratcher (AGC Thecno Glass, Tokyo, Japan), leaving two separated cell monolayers with a cell-free gap that was approximately 2-mm wide. Then, the medium was replaced with GM, OIM-FBS, or OIM-PRGFs. After 72 hours, photographs of hDFCs were taken using a DP12 Microscope Digital Camera (Olympus, Tokyo, Japan). The areas of migratory cells were measured using Image J (National Institutes of Health, Bethesda, MD, USA). Results were expressed as percent of the migratory cell area per mm2.
Dp12 microscope digital camera
Manufactured by Olympus
Sourced in Japan
The DP12 Microscope Digital Camera is a high-quality camera designed for microscopy applications. It captures detailed digital images of microscopic specimens with a maximum resolution of 3.3 megapixels. The camera is compatible with a wide range of microscopes and connects via a USB interface to enable easy integration with computer systems for image capture and analysis.
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Lab products found in correlation
2 protocols using dp12 microscope digital camera
1
Scratch Assay for hDFC Migration
2
Microscopic Evaluation of Blood Parasites
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Blood smears were performed immediately after the blood sample collection. The blood smear was fixed with methanol (Merck®, Germany) until dry. Blood smears were stained with 10% Giemsa stain for approximately 30 min. Morphological examinations were performed using a microscope (OPTIKA®, Italy) at 1000× magnification with immersion oil (Merck®). Images were captured using Dp12 microscope digital camera (Olympus®, Japan) and parasite measurements were conducted using the ImageJ software (National Institutes of Health and the Laboratory for Optical and Computational Instrumentation, University of Wisconsin, USA). Parasitemia levels in blood smear samples were counted only in samples that showed positive results based on the polymerase chain reaction (PCR). Parasitemia levels were determined by counting the number of blood parasites per 2000 red blood cells. Parasitemia levels in blood samples were considered high if there were >10 blood parasites in 500 red blood cells and low if there were <10 blood parasites in 500 red blood cells [7 ].
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