Recoverease dna isolation kit
The RecoverEase DNA Isolation Kit is a laboratory tool designed for the extraction and purification of DNA from various sample types. It provides a simple and efficient method to isolate high-quality DNA suitable for downstream molecular biology applications.
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17 protocols using recoverease dna isolation kit
UV-Induced Mutagenesis in Transgenic Mice
Intestinal DNA Extraction Protocol
Extraction and Phage Recovery from Lung DNA
Isolation of 6-TG-Resistant Mutants
Genomic Sequencing of Taiwanese Insect
Genomic DNA Extraction and Phage Packaging
Bacterial Lacl Mutant Screening
Isolation of High-Quality Genomic DNA
Mutational Analysis of Organoid-Derived DNA
Quantifying gpt Mutation Frequency
the small intestine mucosa by means of a RecoverEase DNA Isolation Kit (Agilent
Technologies, Santa Clara, CA, USA), and lambda EG10 phages were recovered with Transpack
Packaging Extract (Agilent Technologies). Escherichia coli YG6020 were
infected with the recovered phages, plated on M9 salt plates containing chloramphenicol
(Cm) and 6-thioguanine (6-TG), and then incubated for 72–90 h at 37°C. This incubation
enabled selection of colonies harboring a plasmid carrying both the gene for
chloramphenicol acetyltransferase and a mutated gpt gene.
gpt-Mutant frequency was calculated by dividing the number of mutated
colonies growing on agar plates containing Cm and 6-TG by the number of colonies growing
on agar plates containing Cm alone. The mutants exhibiting the 6-TG-resistant phenotype
were cultured overnight at 37°C in Luria–Bertani broth containing 25 µg/mL Cm, harvested
by centrifugation (7000 rpm, 10 min), and then stored at −80°C. A 739-bp DNA fragment
containing gpt was amplified by means of the polymerase chain reaction
and sequenced as described previously12 (link),13 (link)).
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