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Human tslp elisa max deluxe sets

Manufactured by BioLegend
Sourced in United States

The Human TSLP ELISA MAX Deluxe Sets are enzyme-linked immunosorbent assay (ELISA) kits used for the quantitative measurement of human thymic stromal lymphopoietin (TSLP) in biological samples such as cell culture supernatants, serum, and plasma. The kits provide all the essential components required to perform the ELISA assay, including pre-coated plates, detection antibodies, and calibration standards.

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2 protocols using human tslp elisa max deluxe sets

1

Quantifying TSLP and IgA Levels

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Serum levels of TSLP and IgA were measured using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and an IgA Human ELISA Kit (ab137980; Abcam) according to the manufacturers’ protocols. The absorbance was read at 450 and 550 nm using a Varioskan Flash 2.4 System (Thermo Fisher scientific, Waltham, MA, USA). The sensitivity of the assay was 2 pg/mL for TSLP and 1.5 ng/mL for IgA.
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2

Optimizing IgA Production in FDC Clusters

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For IgA production analysis in FDC-associated clusters, the base cell culture media was supplemented with TW at 1 μg/mL, 5 μg/mL, or 10 μg/mL for the initial 7 days of culture. After collection of supernatants, qualitative detection of TSLP and IgA in cell supernatants was performed using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and IgA Human ELISA Kit (ab137980; Abcam).
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