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Atp determination kit

Manufactured by Beyotime
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Sourced in China

The ATP Determination Kit is a laboratory equipment designed to quantify the levels of adenosine triphosphate (ATP) in a sample. It provides a sensitive and reliable method for measuring ATP, which is a fundamental energy currency in living cells.

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Lab products found in correlation

Varioskan flash, by Thermo Fisher Scientific (2 mentions) Jc 1 staining, by Beyotime (2 mentions) Jc 1 mitochondrial membrane potential assay kit, by Beyotime (2 mentions) A1r confocal microscope, by Nikon (1 mentions) Jc 1 dye, by Beyotime (1 mentions) Mitotracker red, by Beyotime (1 mentions) Synergy 2 multi mode microplate reader, by Agilent Technologies (1 mentions) Nadp nadph assay kit, by Beyotime (1 mentions) Microplate luminometer, by Promega (1 mentions) Firefly luciferase, by Beyotime (1 mentions) Cu zn sod and mn sod assay kit with wst 8, by Beyotime (1 mentions) Dihydroethidium, by Beyotime (1 mentions) Varioskan flash fluorescence plate reader, by Thermo Fisher Scientific (1 mentions) Lactate assay kit, by Abcam (1 mentions) β actin 13e5 rabbit mab, by Cell Signaling Technology (1 mentions) Triton x 100 solution, by Beyotime (1 mentions) Rbc lysis buffer, by Solarbio (1 mentions) Western bright peroxide, by Advansta (1 mentions) Cleaved caspase 3 asp175 antibody alexa fluor 488 conjugate, by Cell Signaling Technology (1 mentions) Sulforhodamine b, by Solarbio (1 mentions)

51 protocols using atp determination kit

1

Mitochondrial Staining and ATP Quantification

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hiPSC-CMs were stained with either JC-1 dye (Beyotime, China) or prewarmed MitoTracker Red (0.2 μM; Beyotime, China) for 25 min at 37 °C according to the manufacturers’ instructions. For mitochondrial staining, the nucleus was stained first. Ten microliters Hoechst dye was added to 1 ml of medium, incubated for 16 min at 37 °C, and washed three times with PBS. Then, the mitochondria were stained. The staining and fluorescence intensity of the cells was evaluated using an A1R confocal microscope (Nikon, Japan). The exact amount of ATP is determined by an ATP determination kit (Beyotime, China) according to the manufacturer’s instructions.
Zhang X., Ye L., Xu H., Zhou Q., Tan B., Yi Q., Yan L., Xie M., Zhang Y., Tian J, & Zhu J. (2021). NRF2 is required for structural and metabolic maturation of human induced pluripotent stem cell-derived ardiomyocytes. Stem Cell Research & Therapy, 12, 208.
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2

ATP Quantification Protocol

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ATP measurements were obtained using an ATP determination kit (Beyotime Biotechnology, China).The ATP content was determined based on comparison with a concurrent standard curve.
Lv X.B., Liu L., Cheng C., Yu B., Xiong L., Hu K., Tang J., Zeng L, & Sang Y. (2015). SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer. Scientific Reports, 5, 17940.
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3

Quantifying Cellular ATP Levels

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ATP level was measured using an ATP Determination kit (S0027, Beyotime Biotechnology, China) according to the manufacturer’s protocol. Luminescence was recorded using a luminescence plate reader.
Huan Y., Quan H., Jia B., Hao G., Shi Z., Zhao T., Yuan Y., Yuan F., Dong Y, & Liang G. (2023). High-altitude cerebral hypoxia promotes mitochondrial dysfunction and apoptosis of mouse neurons. Frontiers in Molecular Neuroscience, 16, 1216947.
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4

ATP Quantification in H9C2 Cells

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An ATP determination kit (Beyotime, Nanjing, China) was employed for determining the content of ATP. A reaction buffer (100 ml) supplemented with dithiothreitol (1 μM), luciferase (12.5 mg/ml), and luciferin (0.5 μM) was added into H9C2 cells. A Varioskan Flash microplate reader was applied for measuring the luminance of the mixtures. The ATP content was defined to be the percentage of the total level in the control group.
Chen L., Yan L, & Zhang W. (2022). Benzoylaconine improves mitochondrial function in oxygen-glucose deprivation and reperfusion-induced cardiomyocyte injury by activation of the AMPK/PGC-1 axis. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 26(5), 325-333.
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5

Quantifying Cellular ATP Levels

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The ATP levels were measured using a firefly luciferase-based ATP Determination Kit (Beyotime, S0026). Cells were lysed and centrifuged at 12,000×g for 5 min, the supernatant was mixed with 100 μL ATP detection working solution, and the luminescence value was measured by using a microplate reader (Thermo, Varioskan Flash). The ATP level was expressed as the percentage of the level that was observed in the control groups.
Zhang R., Li G., Zhang Q., Tang Q., Huang J., Hu C., Liu Y., Wang Q., Liu W., Gao N, & Zhou S. (2018). Hirsutine induces mPTP-dependent apoptosis through ROCK1/PTEN/PI3K/GSK3β pathway in human lung cancer cells. Cell Death & Disease, 9(6), 598.
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6

ATP Quantification in Cells

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A total of 1 × 10 4 cells were harvested and incubated with 200 µL cell lysis buffer according to the ATP determination kit (Beyotime). Cells were aspirated and centrifuged at 1200 × g for 5 min. Next, 100 µL supernatants were moved to prepared wells for 5 min. ATP luminescence was detected using a microplate reader (Synergy 2 Multi-Mode microplate reader, BioTek, Winooski, VT).
Lv X., Qiu J., Hao T., Zhang H., Jiang H., & Tan Y. (2021). The Mechanism of Catalpol in Alzheimer’s Disease by Modulating Mitochondrial Function Through miR-124-Mediated STIM2.
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7

Quantifying Cellular ATP Levels

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The firefly luciferase-based ATP Determination Kit (Beyotime Company, S2006) was used to measure ATP levels according to the manufacturer’s instructions. Cells treated with various concentrations of MPP+ were lysed and centrifuged, and ATP detection working solution was added to the supernatant. The luminescence value, which was determined with a microplate reader (Thermo, Varioskan Flash), was used as an index of the ATP level. The ATP level was normalized to that of the control group (100%).
Zhang Q., Hu C., Huang J., Liu W., Lai W., Leng F., Tang Q., Liu Y., Wang Q., Zhou M., Sheng F., Li G, & Zhang R. (2019). ROCK1 induces dopaminergic nerve cell apoptosis via the activation of Drp1-mediated aberrant mitochondrial fission in Parkinson’s disease. Experimental & Molecular Medicine, 51(10), 116.
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8

Quantifying ATP Levels in Biological Samples

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ATP concentration was quantitatively determined using an ATP determination kit (Beyotime, Nanjing, China) according to the manufacturer's protocol. ATP content was expressed as nmol/mg protein.
Zhao C., Chen Z., Qi J., Duan S., Huang Z., Zhang C., Wu L., Zeng M., Zhang B., Wang N., Mao H., Zhang A., Xing C, & Yuan Y. (2017). Drp1-dependent mitophagy protects against cisplatin-induced apoptosis of renal tubular epithelial cells by improving mitochondrial function. Oncotarget, 8(13), 20988-21000.
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9

Measuring ATP and NADPH in Reoxygenation

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The levels of ATP and NADPH in cells were measured 24 h after reoxygenation by using the ATP determination Kit and NADP/NADPH assay kit (Beyotime, Haimen, China) according to the manufacturer's recommendations.
Zeng S., Zhao Z., Zheng S., Wu M., Song X., Li Y., Zheng Y., Liu B., Chen L., Gao C, & Liu H. (2021). The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR. Redox Biology, 45, 102058.
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10

Intracellular ATP Quantification Assay

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After indicated treatment, cells were lysed with RIPA lysis solution, and cell lysate were harvested via centrifugation at 12,000g for 5 min at 4 °C. Intracellular ATP content was tested with an ATP Determination Kit (Beyotime Biotechnology, Shanghai, China) using a luciferase-based assay according to the manufacturer’s instructions. Briefly, cell lysate and ATP standard solutions were diluted with luciferase buffer, respectively. The luminescence was measured using a Microplate Luminometer (GloMax 96; Promega, USA). Finally, ATP level was calculated based on the standard curve.
Kang T., Han Z., Zhu L, & Cao B. (2024). TFR1 knockdown alleviates iron overload and mitochondrial dysfunction during neural differentiation of Alzheimer’s disease-derived induced pluripotent stem cells by interacting with GSK3B. European Journal of Medical Research, 29, 101.
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