For this study, we used the mouse line B6.Cg-Mir146tm1.1Bal/J (stock no. 016239) from Jackson Laboratory. Mice were housed and handled in accordance with current best practices. Their well-being was monitored daily by a contractor as a paid service (Animalliance). Homozygous mice at 6–8 months of age start to develop lymphoproliferative and myeloproliferative diseases, subsequently leading to anemia, thrombocytopenia, and lymphopenia ([25 (link)] and Jackson Laboratory). As such, all molecular and behavioral analyses described in this study were performed before mice reach 6 months old (4 months old at the latest for behavioral tests), before the appearance of any inflammatory disease signs. All experiments performed were approved by an independent ethics committee of Paris Descartes University (approval number 2015101215207380) and declared to the French Ministry of Research.
B6 cg mir146tm1.1bal j
Manufactured by Jackson ImmunoResearch
B6.Cg-Mir146tm1.1Bal/J is a laboratory mouse strain that carries a targeted mutation in the miR-146a gene. This strain is used in research to study the role of miR-146a in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using b6 cg mir146tm1.1bal j
1
Murine Lymphoproliferative Disease Model
2
Generating miR-146a Knockout mdx52 Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
mdx52 mice (dmd−/− or dmd−/Y) on a C57/BL/6 background are bred in house22 (link). miR-146a−/− (B6.Cg-Mir146tm1.1Bal/J) have been previously described23 (link) and were purchased from The Jackson Laboratory (Strain # 016239) and are on a C57/BL/6J background. To generate mdx52 mice with deletion of miR-146a, dmd−/−;146a+/+ females were crossed with dmd+/Y;146a−/− males to generate dmd−/+:146a+/− females and dmd−/Y;146a+/− males. dmd−/+;146a+/− females and dmd−/Y;146a+/− males were then crossed to generate dmd−/−;146a−/− and dmd−/Y;146a−/− breeders. Breeders were crossed to generate dmd−/Y;146a−/− mice (146aX). For all experiments only males were utilized as DMD is an X-linked disorder. Genotyping for mdx52 and miR-146a KO alleles was performed using TransnetYX with custom primers in Dmd exon 3 and a neomycin cassette for mdx52 and with the standard JAX protocol for miR-146a−/− with forward primer 24664 (5′-GCT TAT GAA CTT GCC TAT CTT GTG-3′) and reverse primer 24665 (5′-CAG CAG TTC CAC GCT TCA-3′).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!