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39 protocols using potassium bromide

1

Synthesis of Plasmonic Nanostructures

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Bismuth(III) nitrate pentahydrate (Bi(NO3)3·5H2O), potassium bromide (KBr), titanium(IV) oxide (TiO2), tetrachloroauric(III) acid tetrahydrate (HAuCl4·4H2O), sulfuric acid (H2SO4), and sodium sulfite (Na2SO3) were of analytical grade and supplied by Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). An ultrapure water purification system (Eco‐S15Q, Hitech Instruments Co. Ltd, Shanghai, China) was used to produce 18.2 MΩ cm−1 deionized water (DI water) in all the experiments.
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2

Polyethersulfone Membrane with Vitamin B12

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Polyethersulfone (PES, purity 99%, MV40000) was provided by Jida High-tech Materials Co., Ltd. (Changchun, China). Vitamin B12 (VB12, 1 g) was provided by Saiguo Biotechnology Co., Ltd., Guangzhou, China. Vancomycin hydrochloride (0.1 g) was provided by Shanghai Yuanye Bio-Technology Co., Shanghai, China. Other chemical reagents, such as Potassium bromide (KBr, purity 99%), N,N-Dimethylacetamide (DMAC, purity 99%), polyethylene glycol 400 (PEG400, purity 99%), methanol (CH3OH, purity 99%), concentrated sulfuric acid (H2SO4, purity 99%), polyvinylpyrrolidone (PVP, K30), formic acid (HCOOH, purity 99%), Ceric ammonium nitrate (CAN, purity 99%), acrylic acid (AAC, purity 99%), acrylamide (AAM, purity 99%), and N,N-Dimethylbisacrylamide (BIS, purity 99%) were all provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Reverse osmosis-derived water and deionized water (D.I. water) were used in all experiments.
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3

Irradiated Chitosan Biopolymer for Food Preservation

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The main materials used were natural starch (food grade), ethanol, n-hexane, acetic acid, disodium hydrogen phosphate, citric acid, calcium chloride anhydrous, sodium hydroxide, potassium bromide (KBr), magnesium oxide (MgO), nutrient agar, and carboxymethyl chitosan (CMCS); all were analytically pure materials and purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). Sodium alginate (SA) was purchased from Shanghai Maclean Biochemical Technology Co. (Shanghai, China). BCP (bromocresol purple plate count agar) medium with 0.2% soluble starch was purchased from Beijing Wokai Biotechnology Co. (Beijing, China). Two strains, Aeromonas sobria (ATCC43979) and Shwanella putrefaciens (ATCCBAA-1097), were purchased from Shanghai Preservation Biotechnology Centre (Shanghai, China). Sweet orange essential oil (SOEO) was sourced from sweet orange essential peel. The chitosan used in this experiment was treated with 10 KGY irradiation (Hubei Irradiation Experimental Center (Beijing, China), 60Co-ray; the irradiation dose rate was 6.25 Gy/min, and the irradiation doses were set at 20, 40, 60, 80, and 100 KGy.
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4

Characterization of Freeze-Dried Rose Starch

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The freeze-dried roses were provided by Shandong Huamei Biotechnology Co., Ltd. (Pingyin, Jinan, Shandong). Corn (amylose content, 33%), potato (amylose content, 36%) and pea starch (amylose content, 43%) were purchased from Shandong Fuyang Biological Starch Ltd. All reagents such as absolute ethanol, Folin-Ciocalteu, sodium carbonate, and potassium bromide were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
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5

Fresh Silver Carp Preparation

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Fresh silver carps (Hypophthalmichthys molitrix) were acquired and transported to the lab from a local fish market in Dalian, Liaoning Province, China. After removing the head, scales, and viscera, the fish were rinsed thoroughly in 4 °C deionized water and set away for later use. All chemicals used were of analytical grade except for potassium bromide which is spectrally pure; they were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) or Sigma-Aldrich (St. Louis, MO, USA).
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6

Chitosan Synthesis and Cell Culture

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Chitosan was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The degree of deacetylation was 90% and viscosity-average molecular weight was 5.3 × 104. Other reagents, such as sodium carbonate, acetic acid, ethanol, potassium bromide (KBr), and solvents, were analytical grade and were also supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). FBS (fetal bovine serum) and Dulbecco’s Modified Eagle Medium (DMEM) culture medium were purchased from Gibco Company (Langley, OK, USA). Penicillin–streptomycin for the cell culture, as well as trypsin, were purchased from Beyotime Bio Technology Co., Ltd. (Shanghai, China). MTT (M2128) was supplied by Sigma Company (St. Louis, MO, USA).
Fibroblasts, NCTC clone 929 (mouse connective tissue), were purchased from Cell Resources Center (Shanghai Academy of Life Sciences, Chinese Academy of Sciences, Shanghai, China) and preserved in the School of Pharmaceutical Sciences, Jiangnan University. L929 cells were grown in DMEM supplemented with 10% (v/v) FBS and 100 IU/mL Penicillin–streptomycin. The cells were maintained in a humid incubator (5% CO2) at 37 °C [32 (link)].
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7

Synthesis of Organic Compounds

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1,3-Diphenylguanidine, potassium bromide, deuterated dimethyl sulfoxide, potassium pyrophosphate, potassium oleate, potassium hydroxide, ammonia, sodium fluorosilicate, formaldehyde, ethylene glycol, and ethanol are purchased from Sinopharm Chemical Reagent Co., Ltd. Hydroxypropyl-β-cyclodextrin is purchased from Aladdin Reagent Shanghai Co., Ltd.
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8

Beef-based Gelatin Production Protocol

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Fresh beef was sourced from a local market in Hefei, Anhui province. The gelatin, pullulan polysaccharide, inulin, and whey protein isolate were of food grade quality (Source Leaf Biology (Shanghai, China)). Eu, sodium chloride, sodium hydroxide, hydrochloric acid, boric acid, trichloroacetic acid, potassium bromide, absolute ethanol and PCA medium were of analytical grade (Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China)).
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9

Beef-based Gelatin Production Protocol

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Fresh beef was sourced from a local market in Hefei, Anhui province. The gelatin, pullulan polysaccharide, inulin, and whey protein isolate were of food grade quality (Source Leaf Biology (Shanghai, China)). Eu, sodium chloride, sodium hydroxide, hydrochloric acid, boric acid, trichloroacetic acid, potassium bromide, absolute ethanol and PCA medium were of analytical grade (Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China)).
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10

Konjac Glucomannan and Chemical Reagents

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Konjac glucomannan was purchased from konson konjac Gum Co., Ltd (Hubei, China). Sodium chloride (NaCl), sodium carbonate (Na2CO3) and potassium bromide (KBr) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
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