Acetonitrile

The following chemicals were used in this research: aceton (Lach-Ner, Neratovice, Czech Republic); acetonitrile (Applichem, Darmstadt, Germany); ammonium formate (GramMol, Zagreb, Croatia); argon, 99.9995% (Messer, Bad Soden am Taunus, Germany), certified element reference material (CPAChem, Bogomilovo, Bulgaria); distilled water and nitric acid (Gram-Mol, Zagreb, Croatia); helium, 6.0 (Messer, Bad Soden am Taunus, Germany); methanol (GramMol, Zagreb, Croatia); mixture of magnesium sulphate, sodium chloride, and citrate salts (Citrate-Kit-01, BEKOlut, Bruchmühlbach-Miesau, Germany); mixture of magnesium sulphate salt, primary secondary amine, and GCB (PSA-Kit-06, BEKOlut, Bruchmühlbach-Miesau, Germany); sodium hydroxide (T.T.T., Sveta Nedjelja, Croatia); petrol ether (Applichem, Chicago, IL, USA); sulfuric acid (Chemistry, Zagreb, Croatia); and hydrogen peroxide (Gram-Mol, Zagreb, Croatia).

Ovalbumin (Ova, ≥90%),
α-chymotrypsinogen A (α-CT) from bovine pancreas, myoglobin
(Myo) from equine skeletal muscle (≥95%), horseradish peroxidase
type VI-A (HRP), and β-lactoglobulin (β-LG) from bovine
milk (≥90%) were obtained from Sigma-Aldrich (Steinheim, Germany).
Appropriate amounts of protein powder were dissolved in SEC buffer.
Ultrapure water (MQ) was obtained with a Milli-Q system from Merck
Millipore (Darmstadt, Germany). Trifluoroacetic acid and acetonitrile,
both HPLC-grade, were purchased from AppliChem (Darmstadt, Germany).
All other chemicals used for the preparation of mobile phases were
obtained from Carl Roth (Karlsruhe, Germany).

The reference standard OLEA (≥90%) was purchased from Toronto Research Chemicals (North York, ON, Canada) and hydroxytyrosol (OH-TY) was obtained from Extrasynthese (Genay, France). The reagents methanol, acetonitrile (ACN), and formic acid were acquired from AppliChem, Panreac Quimica SLU (Barcelona, Spain). Ultrapure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).

OLC (≥95% purity) was purchased from Merck (Darmstadt, Germany), and OLE (≥90% purity) and OLA (≥95% purity) from Toronto Research Chemicals (North York, ON, Canada). MeOH, acetonitrile (ACN) and formic acid were acquired from AppliChem, Panreac Quimica SLU (Barcelona, Spain). Hexane, 4-hydroxybenzaldehyde, chloroform-d, tetramethylsilane and gentisic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(4-hydroxy-3-methoxyphenyl)propionic acid was purchased from Fisher Scientific (Waltham, MA, USA) and cycloHexane from Carlo Erba (Madrid, Spain). Ultrapure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).

Acetonitrile, ethanol, methanol, formic acid, and acetic acid were purchased from AppliChem, Panreac Quimica SA (Barcelona, Spain). Hexane, methyl tert-butyl ether (MTBE), all-E-α-carotene, all-E-β-carotene, all-E-lutein, all-E-lycopene caffeic acid, p-coumaric acid, chlorogenic acid, ferulic acid, isolariciresinol, larisiresinol, luteolin, naringenin, oleuropein, pinoresinol, protocatchuic acid, quercetin, rutin, and secoisolariciresinol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Narigenin-7-O-glucoside, hydroxytyrosol, and ethyl gallate were acquired from Extrasynthese (Genay, France), vanillic acid and apigenin from Fluka (St. Louis, MO, USA), and verbascoside from HWI Analytic. C18 ODS SPE bulk sorbent was purchased from Agilent Technologies (Santa Clara, CA, USA). Ultrapure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).

Water was purified using a Milli-Q system (Millipore, Merck Russia, Moscow, Russia). Formic acid (pure, 98+%, Acros organics, Germany), ammonium formate (for LC-MS, Sigma Aldrich, Moscow, Russia), HPLC-grade methanol (LiChrosolv, Merck, Germany), and acetonitrile (Panreac, AppliChem, Germany) were used for the preparation of the standard solutions of mycotoxins and mobile phases. acetonitrile (pure, Criochrom, St. Petersburgh, Russia) was used for sample extraction. The pH of water for tea infusing was adjusted by citric acid monohydrate (≥99.8%, Lenreactiv, St-Petersburg, Russia), sodium hydroxide (>98%, Reakhim, Moscow, Russia), sodium bicarbonate (99.5–100.5%, Sigma Aldrich, Moscow, Russia).
Three types of water were used to prepare infusions: distilled water (DW) (average pH 5.4 (n = 6, CV = 2.1%); total dissolved solids, average TDS = 4.4 mg/L (n = 5, CV = 12%)), bottled deep-well water (D-WW) (average pH 7.9 (n = 6, CV = 6,4%); average TDS = 155 mg/L (n = 5, CV = 0.8%)) and bottled still natural mineral water (NMW) (average pH 7.6 (n = 5, CV = 2.2%); average TDS = 238 mg/L (n = 5, CV = 0.4%)). TDS were measured by portable conductometer COM-360 (HM-digital, Culver City, CA, USA). Bottled water was purchased from the local market.

Docosahexaenoic acid, Docosahexaenoic acid—d5, Eicosapentaenoic acid, Eicosapentaenoic acid—d5, Arachidonic acid, Arachidonic—d8, Linoleic acid, and α-Linolenic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Linolenic Acid—d4 and α-Linolenic acid—d14 were purchased from Cayman Chemical Company (Ann Arbor, MI, USA).
Methanol and Hexane were purchased from Honeywell (Seelze, Germany), potassium hydroxide from Chimreactiv (Bucharest, Romania), acetonitrile from AppliChem GmbH (Darmstadt, Germany), isopropanol, formic acid, and ammonium acetate from Merck (Darmstadt, Germany), and water from VWR International (Radnor, Pennsylvania, USA). All reagents were HPLC-grade.