6538 uhd accurate mass q tof lc ms spectrometer

All the chemicals, reagents, single-stranded oligos (dA₂₀, dT₂₀, dG₂₀, dC₂₀), self-complementary oligos {d(ATAT)₅, (D1)_mix, Drew-AT, Calf-Thymus DNA (CT–DNA), (DM1, DM2, DM3, DM4, DM5, DM6 and DM7)}, RNA, Hoechst 33258 and Cresol Violet were purchased from Sigma–Aldrich. ¹H and ¹³C NMR spectra were recorded on a Bruker AV-400 MHz spectrometer with chemical shifts reported as parts per million (ppm) (in DMSO-d₆, tetramethylsilane as an internal standard) at 20°C. UV-vis absorption and emission spectra were measured in quartz cuvettes of 1 cm path length. High resolution mass spectra (HRMS) were obtained on Agilent Technologies 6538 UHD Accurate-Mass Q-TOF LC/MS spectrometer.

All the chemicals, reagents, self-complementary Drew-AT, Hoechst 33258, phosphate buffer saline (PBS), 2′,7′-dichlorofluorescein (DCFDA), 5-bromo-2′-deoxyuridine (BrdU), doxorubicin (dox), hydrogen peroxide (H₂O₂), tert-butyl hydroperoxide (TBHP), potassium superoxide (KO₂), sodium hypochlorite (NaOCl), diethylamine NONOate sodium salt hydrate, sodium nitrite (NaNO₂) and N-acetyl-l-cysteine (NAC) were purchased from Sigma-Aldrich. All the synthesized compounds were purified by column chromatography using Rankem silica gel (60–120 mesh). ¹H and ¹³C NMR spectra were recorded on a Bruker AV-400 MHz spectrometer with chemical shifts reported as parts per million (ppm) (in CDCl₃, DMSO-d₆, tetramethylsilane as an internal standard) at 20 °C. High resolution mass spectra (HRMS) were obtained on an Agilent Technologies 6538 UHD Accurate-Mass Q-TOF LC/MS spectrometer. The UV-vis absorption and emission spectra were recorded on Agilent Technologies Cary series UV-vis-NIR absorbance and Cary Eclipse fluorescence spectrophotometers, respectively. UV-vis absorption and emission spectra were obtained in quartz cuvettes with a path length of 1 cm.