CaPA preparations were prepared from brain tissue isolated from Acta2-GCaMP5-mCherry mice and incubated with FITC-conjugated isolectin B4 (10 μg/mL) in aCSF for 30 min. Fluorescence was visualized with an Andor Revolution confocal system (Andor Technology, UK) equipped with an upright 10x objective (NA 0.25) and CCD camera.
Revolution confocal system
Manufactured by Oxford Instruments
Sourced in United Kingdom
The Revolution confocal system is a high-performance microscope designed for advanced imaging applications. It provides high-resolution, three-dimensional imaging capabilities that enable researchers to visualize and study complex biological samples with exceptional clarity and detail. The core function of the Revolution confocal system is to capture and process high-quality, distortion-free images through the use of a confocal scanning mechanism and advanced optical components.
Automatically generated - may contain errors
Lab products found in correlation
Axio observer z1, by Zeiss (3 mentions)
Planapo n objective, by Olympus (1 mentions)
Plan apochromat objective, by Zeiss (1 mentions)
Csu x1 spinning disk head, by Yokogawa (1 mentions)
Csu10 spinning disk confocal scanner, by Yokogawa (1 mentions)
Ix81 microscope, by Oxford Instruments (1 mentions)
Slowfade gold antifade reagent, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
4 protocols using revolution confocal system
1
Brain Tissue-Derived CaPA Visualization
2
Brain Tissue-Derived CaPA Visualization
3
Visualizing Kinetochore Dynamics in C. elegans
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Gravid adult hermaphrodite worms were dissected in M9 buffer, early embryos were transferred onto a 2% agarose pad using a mouth pipet, covered with a 22×22 mm coverslip, and imaged at 20°C. To assay kinetochore recruitment, embryos were imaged on an Andor Revolution confocal system (Andor) coupled to a CSU-10 spinning disk confocal scanner (Yokogawa) and an electron multiplication back-thinned charge-coupled device camera (iXon, Andor), using a 100× 1.4 NA Plan Apochromat objective; or with an inverted Axio Observer Z1 (Zeiss) microscope with a CSU-X1 spinning disk head (Yokogawa) and a 100× 1.3 NA Plan Apochromat Lens (Zeiss). A 6×2 μm z-stack was collected every 10 or 20 seconds.
For assaying mitotic timing and chromosome dynamics, one-cell embryos expressing GFP::H2b were imaged on a widefield deconvolution microscope (DeltaVision) connected to a charge-coupled device camera (pco.edge 5.5 sCMOS; PCO) and a 60× 1.42NA PlanApo N objective (Olympus). A 5×2 μm z-stack was collected every 10 seconds.
For assaying mitotic timing and chromosome dynamics, one-cell embryos expressing GFP::H2b were imaged on a widefield deconvolution microscope (DeltaVision) connected to a charge-coupled device camera (pco.edge 5.5 sCMOS; PCO) and a 60× 1.42NA PlanApo N objective (Olympus). A 5×2 μm z-stack was collected every 10 seconds.
4
Exosome Uptake in Recipient Cells
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Exosomes were isolated from transduced cells expressing a red fluorescent protein. Recipient WT cells without a red fluorescent protein were seeded onto glass coverslips at a density of 4
x 10 5 per coverslip. Isolated exosomes were added to coverslips and incubated in a humidification box for 4 hours. Following incubation cells on coverslips were fixed with 4%
Paraformaldehyde. DNA in cell nuclei was then stained using DAPI (Invitrogen) and the slides mounted using slowfade gold anti-fade reagent (Invitrogen). Immunofluorescent Z-stack images (0.1 μm steps) were captured using an Olympus IX81 Microscope fitted with an Andor Revolution Confocal system (Andor, Belfast, Northern Ireland), 60× oil immersion objective lens and an EMCCD Andor iXonEM + camera.
x 10 5 per coverslip. Isolated exosomes were added to coverslips and incubated in a humidification box for 4 hours. Following incubation cells on coverslips were fixed with 4%
Paraformaldehyde. DNA in cell nuclei was then stained using DAPI (Invitrogen) and the slides mounted using slowfade gold anti-fade reagent (Invitrogen). Immunofluorescent Z-stack images (0.1 μm steps) were captured using an Olympus IX81 Microscope fitted with an Andor Revolution Confocal system (Andor, Belfast, Northern Ireland), 60× oil immersion objective lens and an EMCCD Andor iXonEM + camera.
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