Cyanine3 (cy3)

Manufactured by Affinity Biosciences

Sourced in China

Cy3 is a fluorescent dye molecule commonly used in biological research applications. It has an excitation maximum at 550 nm and an emission maximum at 570 nm, making it suitable for a variety of fluorescence-based detection and imaging techniques.

Automatically generated - may contain errors

Lab products found in correlation

Sybr qpcr master mix, by Vazyme (1 mentions) Cd45 fitc, by BioLegend (1 mentions) Cd44 fitc, by BioLegend (1 mentions) Cd90 fitc, by BioLegend (1 mentions) Fitc phalloidin, by Solarbio (1 mentions) Fetal bovine serum (fbs), by Procell (1 mentions) Hiscript 2 first strand cdna synthesis kit, by Vazyme (1 mentions) Reactive oxygen species assay kit, by Solarbio (1 mentions) Dmem f12, by Procell (1 mentions) Cd34 fitc, by Santa Cruz Biotechnology (1 mentions) Aggrecan, by Affinity Biosciences (1 mentions) Calcein am pi double stain kit, by Beyotime (1 mentions) Freezol reagent kit, by Vazyme (1 mentions) 4 6 diamidino 2 phenylindole dapi, by Biosharp (1 mentions) Cell counting kit 8 (cck8), by Biosharp (1 mentions) Mitochondrial membrane potential assay kit with jc 1, by Solarbio (1 mentions) P ampk, by Abcam (1 mentions) Confocal microscope, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Yeasen (1 mentions) Fluorescein isothiocyanate (fitc), by Affinity Biosciences (1 mentions)

2 protocols using cyanine3 (cy3)

Mesenchymal Stem Cell Characterization

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Neochlorogenic
acids were purchased from Selleck.
CD44-FITC, CD45-FITC, and CD90-FITC were purchased from Biolegend
(United States). CD34-FITC was purchased from Santa Cruz Biotechnology
(United States). Aggrecan, Nrf2, HO-1, Nqo-1, Actin, and Cy3 antibodies
were purchased from Affinity Biosciences (China).
Annexin V-fluorescein
isothiocyanate (FITC)/PI Apoptosis Detection Kit was purchased from
KeyGEN BioTECH (China). Reactive oxygen species assay kit and Mitochondrial
Membrane Potential Assay Kit with JC-1 and FITC Phalloidin were purchased
from Solarbio (China). GelMA hydrogel was purchased from Suzhou Yongqinquan
Intelligent Equipment Co., Ltd. Calcein-AM/PI Double Stain Kit was
purchased from Beyotime Biotechnology (China). 4′,6-Diamidino-2-phenylindole
(DAPI) and Cell Counting KIT 8 was purchased from Biosharp (China).
DMEM/F12 and fetal bovine serum were purchased from Procell (China).
Osteogenesis, adipogenesis, and chondrogenesis differentiation kits
were purchased from OriCell(United States). FreeZol reagent kit, Hiscript
II First Strand cDNA Synthesis kit, and SYBR qPCR Master Mix were
purchased from Vazyme Biotech (China).

Fang Y., Hu J., Zou Y., Wang Z., Ye Y, & Zhang C. (2024). Neochlorogenic Acid Combined with Bone Marrow Mesenchymal Stem Cells Encapsulated into GelMA Hydrogel for Transplantation to Repair Intervertebral Disk Degeneration. Biomacromolecules, 25(2), 729-740.

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Immunofluorescence Imaging of p-AMPK and SIRT1

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Cells were prepared in a 6-well plate and fixed with 10% formalin, for 1 h. The fixed cells were washed three times with PBS and permeabilized with 0.3% Triton X-100, for 30 s. They were washed four times with PBS and blocked with 10% normal goat serum (ZSGB-BIO, Beijing, China) for 45 min. After covering with primary p-AMPK (1: 500, Abcam, Cambridge, MA, USA) and SIRT1 (1: 250, Affinity, Jiangsu, China), for 12 h at 4 °C, the cells were incubated with the secondary antibody IgG (H&L), conjugated with FITC (1: 250, Affinity), and the IgG secondary antibody (H&L), conjugated with CY3 (1: 250, Affinity), for 45 min. The cell nuclei were stained with DAPI (Yeasen, Shanghai, China). Then, the fluorescence photographs were acquired, using a confocal microscope (Olympus, Tokyo, Japan).

Chen Y., Li J., Zhang M., Yang W., Qin W., Zheng Q., Chu Y., Wu Y., Wu D, & Yuan X. (2022). 11β-HSD1 Inhibitor Alleviates Non-Alcoholic Fatty Liver Disease by Activating the AMPK/SIRT1 Signaling Pathway. Nutrients, 14(11), 2358.

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