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4 protocols using sulfuric acid

1

Antioxidant Assays Using Chemical Compounds

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Aluminum chloride, quercetin, vanillin, diosgenin, ascorbic acid, 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,7-diclorodihydrofluorescein diacetate (H2DCFDA), butylated hydroxytoluene (BHT), tert-butylhydroperoxide (TBHP), trichloroacetic acid (TCA), ferrozine, and potassium ferricyanide, were purchased from Sigma-Aldrich (St. Louis, MO, USA). The following were also purchased for use: (Ethylenedinitrilo) tetraacetic acid (EDTA) (Neon, São Paulo, SP, Brazil), ferric chloride (Merk, Darmstadt, Hesse, Germany), sulfuric acid (Labsynth, São Paulo, SP, Brazil), sodium phosphate (Quimiobras, Rio de Janeiro, RJ, Brazil), ammonium molybdate (Vetec, Jaragua do Sul, SC, Brazil), Luria Bertani Medium (Kasvi, São José do Pinhais, PR, Brazil), yeast fermente (Kasvi, São José do Pinhais, PR, Brazil), Nematode Growth Medium (NGM): bacteriological agar and pepton (Kasvi, São José do Pinhais, PR, Brazil), cholesterol (Vetec, Jaragua do Sul, SC, Brazil), calcium chloride (Synth, Diadema, SP, Brazil), magnesium sulfate (Sigma-Aldrich, São Paulo, SP, Brazil), and sodium chloride (Sigma-Aldrich, St. Louis, MO, USA).
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2

Carbon Synthesis via Glycerol Conversion

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Carbon was produced utilizing glycerol 99% from Vetec (São Paulo, São Paulo), sulfuric acid >98% from LabSynth (Diadema, São Paulo) and acetone from Cosmoquimica Indústria e Comércio (Barueri, São Paulo). Characterization techniques used KBr 99.5% from Sigma-Aldrich (São Paulo, São Paulo), NaOH 99% from Vetec (São Paulo, São Paulo), NaHCO3 99.5% from Sigma-Aldrich (São Paulo, São Paulo), HCl 36.5–38.0% from Vetec (São Paulo, São Paulo). Catalytic tests were performed with D(-)fructose >99,9% from Sigma Aldrich (São Paulo, São Paulo) and Dimethylsulfoxide >99,9% from Synth (Diadema, São Paulo). All chemicals were used as received and without any treatment.
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3

Synthesis and Purification of Phenylboronic Acid

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Tetrahydrofuran (THF, analytical grade) and acid phenylboronic (PBA, 97% purity) were supplied by Sigma-Aldrich Corp. (Steinheim, Germany); methanol (HPLC grade) was acquired from Tedia Company Inc. (Fairfield, OH, USA); hexane, acetone, sulfuric acid, sodium bicarbonate, sodium sulfate and ammonium sulfate (analytical grade) were purchased from Labsynth (Diadema, SP, Brazil); diethyl ether (HPLC grade) was from Vetec (Duque de Caxias, RJ, Brazil); and ultrapure water was obtained from a Milli-Q Plus system (Millipore, Bedford, MA, USA).
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4

Analytical Standards for PP-3-MCPD and PP-3-MCPD-d5

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Analytical standards of 3-MCPD-1,2-dipalmitoyl ester (PP-3-MCPD) and 3-MCPD-1,2-dipalmitoyl-d 5 ester (PP-3-MCPD-d 5 ) at a purity higher than 98% were purchased from Toronto Research Chemicals (North York, ON, Canada). Stock solutions at a concentration of 0.5 mg ml -1 were prepared by dissolving the standards in tetrahydrofuran (THF, analytical grade).
Other chemicals used in the experiments included: acid phenylboronic (PBA, 97% purity), supplied by Sigma-Aldrich Corp. (Steinheim, Germany); methanol (HPLC grade), acquired from Tedia Company Inc. (Fairfield, OH, USA); tetrahydrofuran (HPLC grade), supplied by JT Baker (USA); hexane, acetone, petroleum ether, diethyl ether, concentrated hydrochloric acid, sulfuric acid, sodium bicarbonate, sodium sulfate, and ammonium sulfate (analytical grade), purchased from Labsynth (Diadema, SP, Brazil); silver nitrate (99.0-100.5% purity) and potassium chromate (≥99.5% purity), acquired from Merck (Darmstadt, Germany); and ultrapure water, obtained from a Milli-Q Plus system (Millipore, Bedforte, MA, USA).
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