Elyra ps 1 superresolution microscopy

Manufactured by Zeiss

Sourced in Germany

The ELYRA PS.1 is a superresolution microscopy system developed by Zeiss. It utilizes Structured Illumination Microscopy (SIM) technology to achieve resolutions beyond the diffraction limit of light, enabling detailed observation of cellular structures and processes.

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Lab products found in correlation

Slowfade diamond antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Celltracker cm dii dye, by Thermo Fisher Scientific (1 mentions) Prolong diamond anti fade mount, by Thermo Fisher Scientific (1 mentions) Zen 2, by Zeiss (1 mentions) Nucblue fixed cell readyprobes reagent, by Thermo Fisher Scientific (1 mentions) Ab56307, by Abcam (1 mentions) Ab175694, by Abcam (1 mentions) Ab150155, by Abcam (1 mentions)

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ELYRA PS.1 (119 citations)

3 protocols using elyra ps 1 superresolution microscopy

Visualizing pGFPns Delivery to HSPCs

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To visualize the delivery of pGFPns to HSPCs, at 24 and 72 hours, HSPCs from the coculture with Cy5-pGFPns were examined by ELYRA PS.1 Superresolution Microscopy (Zeiss). Briefly, cells were first stained with CellTracker CM-DiI Dye (Invitrogen) for lipid membrane staining and seeded onto poly-l-lysine–coated coverslips. After 10 min, cells were fixed with 4% paraformaldehyde for 10 min at room temperature. After washing with PBS thrice, samples were mounted with SlowFade Diamond Antifade Mountant with DAPI (Invitrogen) for imaging.

Kao C.Y, & Papoutsakis E.T. (2018). Engineering human megakaryocytic microparticles for targeted delivery of nucleic acids to hematopoietic stem and progenitor cells. Science Advances, 4(11), eaau6762.

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Immunohistochemical Staining Protocol for Cellular Structures

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4% Paraformaldehyde (PFA). Phosphate Buffered Saline (PBS). Ethanol (50%, 70%, 80%, 95%, 100%). Ethanol: Xylene (2:1, 1:1; 1:2). Xylene (100%). Xylene: Paraffin (2:1, 1:1, 1:2). Paraffin (100%). Paraffin oven. Paraffin cassettes. Rotary Microtome (Leica RM2125 RTS). Superfrost Plus Microscope Slides 15x75x1.0 mm (Fisherbrand, # 12–550 15). TRIS-buffered saline (TBS) plus 0.025% Triton X-100. 1% (Bovine serum albumin) BSA in TBS. Primary Antibodies (Cortactin (ab84208, Abcam), Tks5 (ab118575, Abcam Biotechnology), MMP-14 (ab56307, Abcam). Secondary Antibodies (Donkey F(ab’)2 Anti-Goat IgG H&L (Alexa Fluor 647) (ab150139, Abcam), Donkey F(ab’)2 Anti-Rabbit IgG H&L (Alexa fluoro-568) (ab175694, Abcam), Donkey F(ab’)2 Anti-Mouse IgG H&L 488 (ab150155, Abcam). DAPI (NucBlue Fixed Cell Ready Probes reagent, Life technologies - Catalog# R37606). Distilled water. Harris Hematoxylin solution. Lithium carbonate solution. 5% Eosin solution. Mounting medium (ProLong diamond Antifade Mount, Life technologies, #P36961). Coverslips (22mmX50mm 1.5 oz. cover class, Electron Microscopy Sciences Sciences. Catalog#72204–04. ZEISS ELYRA PS.1 super-resolution microscopy, Quorum Spinning Disk Confocal microscope. Imaging analyzing software: ZEISS ZEN 2.3 and Volocity 6.3.

Goertzen C., Eymael D, & Magalhaes M. (2018). Three-Dimensional Quantification of Spheroid Degradation-Dependent Invasion and Invadopodia Formation. Biological Procedures Online, 20, 20.

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Super-Resolution Microscopy Imaging Protocol

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SR-SIM images were acquired with Elyra PS.1 Super Resolution microscopy (Carl Zeiss, Germany) using a Plan-Apochromat 63×/1.4 Oil (NA of 1.4), 1.6× tube lens with a collar for correction according to coverslip thickness, and immersion medium (Immersol 518F with a refractive index of 1.518). Laser lines at 405, 488, 561, and 647 nm were used for excitation. SIM images (five different angular orientations of illumination for each SIM image) were captured with a cMOS camera (pco.edge 5.5, 2560 × 2160 pixels at 6.5 µm pixel size; 16-bit dynamic range, peak quantum efficiency >60% or equivalent and field of view ∼80 × 80 µm²).

Singh S.P., Raja S, & Mahalingam S. (2020). Viral protein X unlocks the nuclear pore complex through a human Nup153-dependent pathway to promote nuclear translocation of the lentiviral genome. Molecular Biology of the Cell, 31(4), 304-317.

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