Rabbit anti human β actin polyclonal antibody

RPMI-1640 cell culture medium and fetal bovine serum (FBS) were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Metformin, cisplatin, adenosine 5-monophosphate (AMP)-activated protein kinase (AMPK) signaling pathway blocker compound C and methyl thiazolyl tetrazolium assay (MTT) cell proliferation assay kit were purchased from Sigma (Merck & Co., Inc., Whitehouse Station, NJ, USA). Annexin V-FITC apoptosis detection kit was from Bogu Biological Science and Technology Co., Ltd. (Shanghai, China). Rabbit anti-human AMPK and p-AMPK polyclonal antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA) (dilution, 1:1,000; cat. nos. 2532 and 4184). Rabbit anti-human β-actin polyclonal antibody and goat anti-rabbit horseradish perox-idase-labeled secondary polyclonal antibody were purchased from Boster Biological Technology Co., Ltd. (Pleasanton, CA, USA) (dilution, 1:2,000; cat. nos. BM0627 and BA1054). RIPA protein lysate was purchased from Beijing Kangwei Century Biotechnology Co., Ltd. (Beijing, China).

Human breast cancer cell line MCF-7 and SK-BR-3 was a gift from Dr. Song at Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, USA. Tea Polyphenols was purchased from Sigma (USA). The 5-fluorourocil was purchased from Shanghai Xudong Pharmaceutical. Thiazolyl blue tetrazolium bromide was purchased from Fluka. Rabbit anti- human β-actin polyclonal antibody was purchased from Boster (Wuhan, China). Rabbit anti-human Cyclin D1, Cyclin E, PCNA and Survivin polyclonal antibody was purchased from Santa Cruz (USA).