LC-ESI-MS was
performed on a LXQ system (Thermo Scientific) with
an Agilent Poroshell 300SB-C8 column (5 μm, 75 × 1 mm).
Fc samples were reduced in 40 mM tris (2-carboxyethyl) phosphine at
37 °C for 20 min before LC-ESI-MS measurement. LC separation
of the resulting monomers was performed at 40 °C eluting with
a linear gradient of 20–40% acetonitrile containing 0.1% formic
acid within 10 min at a flow rate of 0.25 mL/min. Percent glycan transfer
was determined by comparing mass spectral abundances of oxime-GlcNAc
Fc2 and oxime-(S2/G2) Fc2.
performed on a LXQ system (Thermo Scientific) with
an Agilent Poroshell 300SB-C8 column (5 μm, 75 × 1 mm).
Fc samples were reduced in 40 mM tris (2-carboxyethyl) phosphine at
37 °C for 20 min before LC-ESI-MS measurement. LC separation
of the resulting monomers was performed at 40 °C eluting with
a linear gradient of 20–40% acetonitrile containing 0.1% formic
acid within 10 min at a flow rate of 0.25 mL/min. Percent glycan transfer
was determined by comparing mass spectral abundances of oxime-GlcNAc
Fc2 and oxime-(S2/G2) Fc2.