Calcein

SPC (purity ≥ 98%, lot no. SY-SI-190601) was obtained from Lipoid (Ludwigshafen am Rhein, Germany). Cholesteryl oleate (CO, purity ≥ 85%), glycerol trioleate (GT, purity ≥ 97%, lot no. D1915109), DHA (purity ≥ 98%, lot no. J1818126), and sodium 1-hexanesulfonate (purity ≥ 98%) were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). CQ (purity ≥ 98%, lot no. C10331390) and cyanine-5.5 (purity ≥ 95%, lot no. C11249895) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Calcein (lot no. LJC0R33) was obtained from J&K Chemical Co., Ltd. (Beijing, China). DOPE, DOPC, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG₂₀₀₀, lot no. RJ0190413), and DSPE-PEG₂₀₀₀-cRGD (lot no. RJ0190813) were provided by Xi’an ruixi Biological Technology Co., Ltd. (Shaanxi, China). Chromatographic grade organic reagents were obtained from Aladdin Reagent Co., Ltd.

Poly(ethylene glycol) (PEG, Mw ~8000), Poly(vinyl alcohol) (PVA, Mw: 89,000–98,000, 99% hydrolyzed), Poly(2-ethyl-2-oxazoline) (PEtOx, Mw~50, 000), sodium citrate, Poly(ethylene glycol) diacrylate (PEGDA, Mn~575), sodium alginate, 2-Hydroxy-4′-(2-hydroxyethoxy)−2-methylpropiophenone (Irgacure 2959), N,N′-methylenebisacrylamide (MBAA), were purchased from Sigma-Aldrich. Dextran (DEX, Mw ~10,000, 40,000, and 500,000) and Nile red were purchased from Macklin. Acrylamide and lithium phenyl-2,4,6 trimethylbenzoylphosphinate (LAP) were purchased from TCI. Rhodamine 6 G, Fluorescein isothiocyanate (FITC) labeled Poly-l-lysine (FITC-PLL, Mw ~30,000–70,000), and Fluorescein isothiocyanate–dextran (FITC-dextran, Mw ~40, 000) were purchased from Sigma-Aldrich. Rhodamine-labeled PEG (Rh-PEG, Mw ~40,000) was purchased from Aladdin. Nucleic acid sequences of Cy5-DNA were synthesized by Integrated DNA Technology (IDT). Calcein was purchased from J&K scientific. Millipore Milli-Q water (18.2 MΩ, pH = 7) was used in all experiments. All materials were used as received without further purification.