Lactate assay kit

Information of antibodies was documented in Table S1. DOX and verteporfin were from MedChem Express (USA). TUNEL stain kit, CCK-8 kit, DHE and DCF reagents were purchased from Beyotime Institute of Biotechnology (Shanghai, China). ATP assay kits was obtained from Jiancheng Bioengineering Institute (Nanjing, China). ELISA kits for plasma cTnI and Gal-3 determination and lactate assay kit were from Elabscience Biotechnology Co., Ltd (Wuhan, China).

Antibodies against NF-κB (#8242), p-NF-κB (#3033), SIRT1 (#8469), and Histone H3 (#4499) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against STING (#A21051) were purchased from ABclonal Technology (Wuhan, China). Antibodies against Nrf2 (SC-365949) were purchased from Santa Cruz Biotechnology (Shanghai, China). Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) was purchased from OriGene Technology (#TA326711, Rockville, MD, USA). Antibodies against GAPDH were purchased from Sigma-Aldrich (#9545, St. Louis, MO, USA). The dilution factor of the antibodies was ascertained according to the manufacturer’s manual. NMN was purchased from Aladdin (#N131850, Shanghai, China). A lactate assay kit was purchased from Elabscience (#E-BC-K044, Wuhan, China). A protein carbonyl assay kit was purchased from Abcam (#ab178020, Boston, MA, USA).

Huh-7 cells at logarithmic growth stage were inoculated into a 96-well plate with density of 1 × 10⁴ cells/well. After indicated transfection for 24 h, the supernatant of the cells in each group was taken, and the levels of glucose and lactate in the culture supernatant was, respectively, detected with the glucose assay kit (Elabscience) and lactate assay kit (Elabscience).