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Peroxidase conjugated anti mouse and anti rabbit antibodies

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Peroxidase-conjugated anti-mouse and anti-rabbit antibodies are secondary antibodies that are conjugated with the enzyme peroxidase. These antibodies are commonly used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and quantify target proteins or molecules in biological samples.

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Lab products found in correlation

Mouse anti akt, by BD (1 mentions) Mouse anti pan erk, by BD (1 mentions)

Spelling variants of this product

Horseradish-peroxidase conjugated goat anti-mouse and goat anti-rabbit secondary antibodies (6 citations) Anti-rabbit antibodies (5 citations) Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse antibodies (2 citations) Anti-mouse and anti-rabbit immunoglobulin G (IgG) horseradish peroxidase-conjugated antibodies (2 citations) Horseradish peroxidase (HRP)‐conjugated polymer anti‐rabbit and anti‐mouse antibodies (2 citations) Anti-TM (1 citations)

2 protocols using peroxidase conjugated anti mouse and anti rabbit antibodies

1

Immunoblot Protein Detection Techniques

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Immunoblots were performed as described [27 (link)]. Anti-GADPH was purchased from Calbiochem. Rabbit anti-human HCK was purchased from Millipore. From BD bioscience, mouse anti-pan ERK, mouse anti-AKT and mouse anti-AKT pT308. From Cell Signaling, rabbit anti-mouse T202/Y204 p-P44/42 MAPK, phospho-pan-PKC rabbit mAb. Peroxidase-conjugated anti-mouse and anti-rabbit antibodies were purchased from DAKO. Membranes were stripped and re-probed with antibodies for total and phosphorylated proteins, when required.
Gato-Cañas M., de Morentin X.M., Blanco-Luquin I., Fernandez-Irigoyen J., Zudaire I., Liechtenstein T., Arasanz H., Lozano T., Casares N., Chaikuad A., Knapp S., Guerrero-Setas D., Escors D., Kochan G, & Santamaría E. (2015). A core of kinase-regulated interactomes defines the neoplastic MDSC lineage. Oncotarget, 6(29), 27160-27175.
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2

Immunoblot Detection of iNOS, GAPDH, and P450R

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Immunoblots were performed as described, using the iNOS-specific antibodies [33 (link)]. Mouse anti-iNOS antibodies were purchased from Cell Signaling, and anti-GADPH from Calbiochem. Polyclonal anti-P450R antibodies were purchased from Abcam. Peroxidase-conjugated anti-mouse and anti-rabbit antibodies were purchased from DAKO.
Liechtenstein T., Perez-Janices N., Gato M., Caliendo F., Kochan G., Blanco-Luquin I., Van der Jeught K., Arce F., Guerrero-Setas D., Fernandez-Irigoyen J., Santamaria E., Breckpot K, & Escors D. (2014). A highly efficient tumor-infiltrating MDSC differentiation system for discovery of anti-neoplastic targets, which circumvents the need for tumor establishment in mice. Oncotarget, 5(17), 7843-7857.
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