AST (Albumin-floxStop-SV40 large T antigen [Tag]) mice were provided by N.G. and G.J.H. from the German Cancer Research Center (Stahl et al., 2009 (link)). TCRSV40-I transgenic (B6.Cg-Tg(TcraY1,TcrbY1)416Tev/J) (Staveley-O’Carroll et al., 2003 (link)), Cre-ERT2 (B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/ J), Alb:Cre (B6.Cg-Tg(Alb-cre)21Mgn/J), C57BL/6J Thy1.2, and Thy1.1 (B6.PL-Thy1a/CyJ) mice were purchased from The Jackson Laboratory. TCRSV40-I mice were crossed to Thy.1.1 mice in our animal facility to generate TCRSV40-I Thy.1.1 mice. AST mice were crossed to Cre-ERT2 or Alb:Cre mice to obtain ASTxCre-ERT2 and ASTxAlb:Cre mice, respectively. OT-I TCR transgenic mice (Hogquist et al., 1994 (link)) were a gift from M. Bevan (University of Washington). Ly5.1 mice were purchased from The Jackson Laboratory. OT-I TCR transgenic mice were crossed to Ly5.1 mice to generate OT-I Ly5.1 mice. All mice were bred and maintained in a specific-pathogen-free barrier facility at the University of Washington and in the animal facility at Memorial Sloan Kettering Cancer Center. Experiments were performed in compliance with the University of Washington and Memorial Sloan Kettering Cancer Center Institutional Animal Care and Use Committee regulations.
Ly5.1 mice
Manufactured by Jackson ImmunoResearch
Ly5.1 mice are a strain of laboratory mice with a specific genetic marker. They express the Ly5.1 allele, which is a cell surface antigen. These mice can be used in various immunological research applications that require the Ly5.1 marker.
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Lab products found in correlation
2 protocols using ly5.1 mice
1
Murine Models for T Cell Immunology
2
Genotyping Gata1 Mutant Mice
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Gata1Δex2 mice (referred to as Gata1s or G1s), which have a deletion of exon 2 that results in unique expression of GATA1s was provided by Stuart Orkin (Children’s Hospital, Boston, MA). For genotyping, tail tips were collected at weaning, digested, and then the recovered DNA was amplified using primers sets wild-type (WT) (forward 5′-AACCCTTTCTGTCCTC-3′, reverse 5′-TCTATGGCAACCCTGA-3′) and G1s (forward 5′-GATGGAGTAGTAGAGG-3′, reverse 5′-TAGCATAAGGTGAGCC-3′). Ly5.1 mice were ordered from The Jackson Laboratory (stock number. 002014). Mice were housed in a barrier facility. Animal studies were approved by the St. Jude Children’s Research Hospital Institutional Animal Care and Use Committee.
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