DNA was extracted from both initial mixtures (0-h incubation) and end point co-cultures (72-h incubation) using the DNeasy Power Biofilm extraction kit (DNeasy PowerBiofilm, Qiagen, Mississauga, ON) as described previously [5 (link)], except that both planktonic (i.e. supernatant of each well) and biofilm fractions (scraped with a pipette tip from the bottom of the 96-well plates) were combined for DNA extraction. Negative controls containing kit reagents only were included for all batches of DNA extractions to identify any systemic contamination of reagents and control for cross-contamination, and these controls were carried through the PCR, library preparation and sequencing process.
Dneasy power biofilm extraction kit
Manufactured by Qiagen
The DNeasy Power Biofilm extraction kit is a laboratory tool designed to extract DNA from biofilm samples. It is intended to facilitate the isolation and purification of DNA for further analysis or processing.
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Lab products found in correlation
2 protocols using dneasy power biofilm extraction kit
1
Comprehensive DNA Extraction from Biofilm and Planktonic Fractions
2
DNA Extraction from Planktonic and Biofilm Fractions
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DNA was extracted from both initial mixtures (0 h incubation) and end point co-cultures (72 h incubation) using the DNeasy Power Biofilm extraction kit (DNeasy PowerBiofilm, Qiagen, Mississauga, ON) as described previously [5] (link), except that both planktonic (i.e. supernatant of each well) and biofilm fractions (scraped from the bottom of the 96-well plates) were combined for DNA extraction. Extraction controls (reagents only) were included for all batches of DNA extractions and these controls were carried through the PCR, library preparation and sequencing process.
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