Soluble cd14

Manufactured by R&D Systems

Sourced in United States

Soluble CD14 is a protein that is naturally present in the human body and plays a role in the immune response. It is a component of the innate immune system and can be detected in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti cx3cr1 apc, by BioLegend (1 mentions) Anti cd14 fitc, by Thermo Fisher Scientific (1 mentions) Anti ccr5 apc cy7, by BD (1 mentions) Anti cd16 pecy7, by BioLegend (1 mentions) Lsrii flow cytometer, by BD (1 mentions) I fabp, by R&D Systems (1 mentions) Neopterin, by Aviva Systems Biology (1 mentions) Microplate reader, by BMG Labtech (1 mentions) Interleukin 4 (il 4), by BD (1 mentions) Fixable viability dye efluor 450, by Thermo Fisher Scientific (1 mentions) Multiskan fc microplate photometer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Soluble CD14 (sCD14) Soluble (s)CD14 Human soluble CD14 Quantikine ELISA Kit

8 protocols using soluble cd14

Quantifying Inflammatory Markers in HIV-related Cardiovascular Disease

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

We focused on inflammatory and immune activation markers that have been associated with stroke and cardiovascular disease in HIV and non-HIV populations[28 (link)–37 (link)]. We tested inflammatory and immune activation markers in cryopreserved biospecimens from the most recent WIHS visit preceding the TCD study. IL-6 and CRP were measured in plasma samples using a multiplex electrochemiluminescence assay (Meso Scale Discovery, MD, USA). Soluble CD14 (R&D Systems, MN, USA) and CD163 (Aviscera Bioscience, CA, USA) were measured by ELISA. Details of peripheral blood mononuclear cell (PBMC) laboratory testing have been described previously[38 (link)]. Cryopreserved PBMCs were thawed in batches and stained with viability dye LIVE/DEAD® Fixable Blue Dead Cell Stain Kit (Life Technologies, NY, USA). Cells were washed and stained with fluorescent conjugated antibodies for cell surface markers. To measure CD4+ and CD8+ T cell activation and identify monocytes, PBMCs were stained as described previously[38 (link)]. Subpopulations of monocytes were evaluated with stains for anti-CD14 FITC (eBioscience, CA, USA), anti-CD16 PE-Cy7 (Biolegend, CA, USA), anti-CCR2 PerCPCy5.5 (Biolegend), anti-CX3CR1 APC (Biolegend), anti-CD163 PE (R&D systems) and anti-CCR5 APC-Cy7 (BD, NJ, USA). Cellular markers were detected by flow cytometry using LSRII flow cytometer (BD).

Chow F.C., Ma Y., Manion M., Rupert A., Lambert-Messerlian G., Bushnell C.D., Cedars M.I., Sereti I., Sorond F.A., Hsue P.Y, & Tien P.C. (2021). Factors associated with worse cerebrovascular function in aging women with and at risk for HIV. AIDS (London, England), 35(2), 257-266.

+ Open protocol

+ Expand

Biomarkers of Gut Damage and Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Circulating proteins associated with an increased plasma lipopolysaccharide content were assessed by measuring lipopolysaccharide-binding protein (Cell Sciences, Inc., Canton, MA) and soluble CD14 concentrations (R&D Systems, Inc., Minneapolis, MN) via an Enzyme Linked ImmunoSorbent Assay (ELISA) following the manufacturer’s instructions. Serum concentration of intestinal fatty acid binding protein (Hycult Biotech, Inc., Wayne, PA) and fecal calprotectin (Genova Diagnostics, Ashville, NC) also were detected by an ELISA following the manufacturer’s instructions and were used as markers of gut damage and inflammation, respectively. All measurements were performed in duplicate.

Alemán J.O., Henderson W.A., Walker J.M., Ronning A., Jones D.R., Walter P.J., Daniel S.G., Bittinger K., Vaughan R., MacArthur R., Chen K., Breslow J.L, & Holt P.R. (2021). Excess dietary fructose does not alter gut microbiota or permeability in humans: A pilot randomized controlled study. Journal of Clinical and Translational Science, 5(1), e143.

+ Open protocol

+ Expand

Comprehensive Evaluation of Liver Disease in SCD

Check if the same lab product or an alternative is used in the 5 most similar protocols

A cohort of SCD patients followed prospectively in a natural history protocol at the National Institutes of Health (NIH) was evaluated (NCT00081523 & NCT00001971). All co-authors had access to the data and reviewed and approved the final manuscript. Baseline demographic, clinical and laboratory data were collected. Patients with suspected liver-related complications of SCD were referred for complete hepatologic evaluation by the hepatology consult service between January 2005 through July 2008. Reasons for referral included persistent elevation of alanine aminotransferase (ALT), alkaline phosphatase (ALP) or direct bilirubin (DB), elevation of ferritin or other iron parameters, and evidence of viral hepatitis. Because the focus was on chronic liver injury, all laboratory analyses were performed at least 2 weeks after or before any acute illness. If clinically indicated, patients were offered liver biopsy. Liver biopsies were preferentially performed by the transjugular route which allowed for estimation of the hepatic venous pressure gradient (HVPG). Hepatic iron load was evaluated (see supplemental methods) and soluble CD14 was measured according to the manufacturer’s protocol (R&D systems, Minneapolis, MN, USA).

Feld J.J., Kato G.J., Koh C., Shields T., Hildesheim M., Kleiner D.E., Taylor J.G., Sandler N.G., Douek D., Haynes-Williams V., Nichols J.S., Hoofnagle J.H., Liang T.J., Gladwin M.T, & Heller T. (2015). Liver Injury is Associated with Mortality in Sickle Cell Disease. Alimentary pharmacology & therapeutics, 42(7), 912-921.

+ Open protocol

+ Expand

Plasma Protein Quantification Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma protein quantifications were performed using commercial kits according to the manufacturers’ instructions: soluble CD14 (R&D Systems, Minneapolis, Minnesota, USA), soluble CD163 (Invitrogen, Thermo-Fisher Scientific), Intestinal-fatty acid binding protein (I-FABP, R&D Systems), LPS-binding protein (LBP, R&D Systems), and soluble CD40L (Invitrogen, Thermo-Fisher Scientific). Experimental duplicates were performed for each sample.

Underwood M.L., Park B., Uebelhoer L.S., Gu G., Kunkel L.E., Korthuis P.T., Cook R.R., Sekaly R.P., Ribeiro S.P, & Lancioni C.L. (2022). Chronic Alcohol Exposure Among People Living with HIV Is Associated with Innate Immune Activation and Alterations in Monocyte Phenotype and Plasma Cytokine Profile. Frontiers in Immunology, 13, 867937.

+ Open protocol

+ Expand

Biomarkers of Intestinal Dysfunction in EED

Check if the same lab product or an alternative is used in the 5 most similar protocols

A range of biomarkers were selected to characterise four domains of EED [12] (link). Briefly, we measured markers of (1) intestinal inflammation (stool neopterin and myeloperoxidase), (2) small intestinal damage and repair (plasma intestinal fatty acid binding protein (I-FABP), plasma citrulline, stool regenerating gene 1β (REG-1B)), (3) intestinal permeability (stool alpha-1 antitrypsin (A1AT)), and (4) microbial translocation and systemic inflammation (plasma soluble CD14, kynurenine:tryptophan ratio (KTR), and C-reactive protein (CRP)). Plasma samples were tested by ELISA according to manufacturers’ instructions for CRP (limit of detection (LOD) 0.01 ng/mL), soluble CD14 (LOD 125pg/mL) (both R&D Systems, Minneapolis, MN, USA); and I-FABP (LOD 47pg/mL) (Hycult Biotechnology, Uden, The Netherlands). Plasma citrulline (LOD 100 ng/mL), kynurenine (LOD 40 ng/mL), and tryptophan (200 ng/mL) were assayed by ultrahigh-performance liquid chromatography tandem mass spectrometry with electrospray ionisation (Waters, Wilmslow, U.K.) at Imperial College, London.
Stool samples were tested by ELISA according to manufacturers’ instructions for neopterin (LOD 0.7 nmol/L; GenWay Biotech Inc, San Diego, USA), myeloperoxidase (LOD 1.6 ng/mL; Immundianostik, Bensheim, Germany), A1AT (LOD 1.5 ng/mL; BioVendor, Brno, Czech Republic), and REG‐1β (LOD 0.625 ng/mL; TECHLAB Inc, Blacksburg, USA).

Church J.A., Rukobo S., Govha M., Gough E.K., Chasekwa B., Lee B., Carmolli M.P., Panic G., Giallourou N., Ntozini R., Mutasa K., McNeal M.M., Majo F.D., Tavengwa N.V., Swann J.R., Moulton L.H., Kirkpatrick B.D., Humphrey J.H, & Prendergast A.J. (2021). Associations between biomarkers of environmental enteric dysfunction and oral rotavirus vaccine immunogenicity in rural Zimbabwean infants. EClinicalMedicine, 41, 101173.

+ Open protocol

+ Expand

Plasma Cytokine Quantification in Surgery

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood collected at the time of surgery were separated for plasma by centrifugation at 1,200 x g for 10 min at 4°C. Plasma at two different dilutions were used for ELISA quantification of lipopolysaccharide-binding protein (1:1,000; Hycult Biotech) and soluble CD14 (1:500 dilution; R&D Systems). Assays were carried out as per manufacturer’s instructions and measured colorimetrically by a microplate reader (BMG LABTECH).

Ha C.W., Martin A., Sepich-Poore G.D., Shi B., Wang Y., Gouin K., Humphrey G., Sanders K., Ratnayake Y., Chan K.S., Hendrick G., Caldera J.R., Arias C., Moskowitz J.E., Ho Sui S.J., Yang S., Underhill D., Brady M.J., Knott S., Kaihara K., Steinbaugh M.J., Li H., McGovern D.P., Knight R., Fleshner P, & Devkota S. (2020). Translocation of Viable Gut Microbiota to Mesenteric Adipose Drives Formation of Creeping Fat in Humans. Cell, 183(3), 666-683.e17.

+ Open protocol

+ Expand

Serum Biomarkers in Cardiovascular Disease

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All blood samples were drawn after a 12-hour fast. Serum oxLDL was measured by enzyme-linked immunosorbent assay (ELISA) (Mercodia, Uppsala, Sweden). The intra and inter-assay variability were 6.3% and 4.7%, respectively. Soluble CD163 (Trillium Diagnostics, intra-assay variability 3–6% and inter-assay variability 5–8%)) and soluble CD14 (R&D Systems, intra-assay variability 4.8–6.4% and inter-assay variability 4.8–7.4%) were measured by ELISA. Direct LDL, total cholesterol, high-density lipoprotein (HDL), triglycerides, glucose, hemoglobin A1c, and lipoprotein-associated phospholipase A₂ (Lp-PLA₂) were measured as previously described [19 (link)].

NOU E., LU M.T., LOOBY S.E., FITCH K.V., KIM E.A., LEE H., HOFFMANN U., GRINSPOON S.K, & LO J. (2016). Serum Oxidized LDL Decreases in Response to Statin Therapy and Relates Independently to Reductions in Coronary Plaque in Patients with HIV. AIDS (London, England), 30(4), 583-590.

+ Open protocol

+ Expand

Profiling Soluble Factors in Skin Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supernatants of skin explant cultures or MonoDCs were collected and analyzed for TSLP (eBioscience)
and soluble CD14 (R&D Systems) production, respectively. After MLR, 10 5 T cells were restimulated with PMA/ionomycin for 1 day and supernatants were evaluated for IL-4 production (BD-Biosciences). ELISA were performed in duplicates according to the manufacturer's recommendations.
The absorbance was detected at 450nm using a Thermo Fisher Scientific Multiskan FC Microplate Photometer. Dead cells stained by Fixable Viability Dye-eFluor450 (eBioscience) were excluded from analysis.

Brulefert A., Hoste A., Muller Q., Fauny J.D., Mueller C.G, & Flacher V. (2021). Vitamin D3-elicited CD14+ human skin dendritic cells promote thymic stromal lymphopoietin-independent type 2 T-helper responses. Allergy, 76(7).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!