4 chlorobenzenesulfonate salt

Manufactured by Thermo Fisher Scientific

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4-chlorobenzenesulfonate salt is a chemical compound used as a reagent in laboratory settings. It serves as a precursor for various synthetic reactions and analysis techniques. The compound provides a source of the 4-chlorobenzenesulfonate ion, which can be utilized in research and development applications.

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1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4 Chlorobenzenesulfonate Salt (DiD) (6 citations) 4-chlorobenzenesulfonate salt (DiD) (5 citations) 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,4-chlorobenzenesulfonate salt (DiD) cell-labeling solution (2 citations) 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine,4 Chlorobenzenesulfonate Salt (DiD) dye (2 citations)

3 protocols using 4 chlorobenzenesulfonate salt

Fluorescent Labeling of Cell Membranes

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Chemicals including chitosan oligosaccharide lactate (Mw = 5000), dithiothreitol (DTT), and acetone were purchased from Sigma-Aldrich. Fluorophores including 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD, excitation/emission = 644/665 nm) and 3,3’-dioctadecyloxacarbocyanine perchlorate (DiO, excitation/emission = 484/501 nm) were purchased from ThermoFisher Scientific. Poly (lactic-co-glycolic) acid (PLGA, 50:50, 0.67 dL/g) was purchased from LACTEL Absorbable Polymers. Packed human red blood cells (RBCs) were purchased from ZenBio, Inc., from which cell membrane was derived according to a previously published protocol.^{[14 (link)]}

Zhang Y., Gao W., Chen Y., Escajadillo T., Fang R.H., Nizet V, & Zhang L. (2017). Self-Assembled Colloidal Gel Using Cell Membrane-Coated Nanosponges as Building Blocks. ACS nano, 11(12), 11923-11930.

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Lipid Probes for Membrane Characterization

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The lipids DOPC, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), DOPS, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidomethyl)cyclohexane-carboxamide] (16:0 MCC-PE), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (DSPE-PEG-Biotin) were obtained from Avanti Polar Lipids (Alabaster, AL). Alexa Fluor 488 SDP ester, Alexa Fluor 594 C5 maleimide, and the lipid fluorophore 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD), and BODIPY-FL-DHPE (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionyl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) were from ThermoFisher Scientific (USA). Streptavidin-conjugated microsphere beads (6 μm diameter) were from Polysciences (Warrington, PA). All common reagents used for the buffer preparation, such as HEPES, Tris, NaCl, Na₂HPO₄, NaH₂PO₄, ethylenediaminetetraacetic acid (EDTA), and dithiothreitol (DTT), were from Fisher Scientific (USA). β-casein from bovine milk was obtained from Sigma-Aldrich (USA). Unless otherwise specified, all chemicals from commercial sources were used without further purification.

Mondal S., Narayan K.B., Powers I., Botterbusch S, & Baumgart T. (2020). Endophilin recruitment drives membrane curvature generation through coincidence detection of GPCR loop interactions and negative lipid charge. The Journal of Biological Chemistry, 296, 100140.

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Lipid Nanoparticle Formulation and Characterization

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Hydrogenated soya phosphatidylcholine (HSPC) and methoxypolyethyleneglycol (MW 2000)-distearylphosphati-dylethanolamine (mPEG2000-DSPE) were purchased from Lipoid (Ludwigshafen, Germany), maleimide-PEG2000 distearoylphosphatidylethanolamine (Mal-PEG2000-DSPE) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate (DiO), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, and 4-chlorobenzenesulfonate salt (DiD) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ammonium sulfate, cholesterol, α-tocopherol, XTT, PMS and DXR hydrochloride were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). TAT peptide analog equipped with 3G as spacer and a C for conjugation (CGGG-RK-KRRQRRRGYG) was synthesized by Peptron Inc. (Daejeon, South Korea) with purity of 98%. Commercially available Caelyx was purchased from pharmacy. All other solvents and reagents were used as chemical grade.

Amin M., Bagheri M., Mansourian M., Jaafari M.R, & ten Hagen T.L. (2018). Regulation of in vivo behavior of TAT-modified liposome by associated protein corona and avidity to tumor cells. International Journal of Nanomedicine, 13, 7441-7455.

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