The neurons or synaptosomes were treated with 21% O2, 5% CO2, and 4.1% sevoflurane [2 minimum alveolar concentration (MAC)] for 4 hours as described by Dong et al. 49 (link). We used an anesthesia machine to deliver 21% O2, 5% CO2, and 4.1% sevoflurane to a sealed plastic box in a 37 °C incubator containing six-well plates seeded with the neurons. A gas analyzer (Dash 4000; GE Healthcare, Milwaukee, WI) was used to continuously monitor the concentrations of delivered carbon dioxide, oxygen, and sevoflurane as performed in our previous studies 49 (link). In the interaction studies, 50 μM MG132 50 (link),51 (link) or 10 μM Nutlin-3 52 (link),53 (link) was administrated to the neurons 1 hour before the sevoflurane treatment. Although different in vitro experiments were performed at different times, the anesthesia and control experiments always occurred concurrently, aiming to reduce bias.
Dash 4000
Manufactured by GE Healthcare
Sourced in United States
The Dash 4000 is a compact patient monitor designed for use in a variety of healthcare settings. It provides continuous monitoring of key vital signs, including heart rate, oxygen saturation, blood pressure, and temperature. The Dash 4000 features a user-friendly interface and intuitive controls to enable efficient patient monitoring.
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Lab products found in correlation
9 protocols using dash 4000
1
Sevoflurane Neurotoxicity Study Protocol
2
Assessing Mandibular Anesthesia Efficacy
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Following the protocol of Vongsavan et al.7 PBF of the mandibular first molars and canines were measured prior to IANB injection as the baseline recordings. Thereafter, PBF changes were monitored at 1, 5, 10, 15, 20, 25, 30 min post injection, and afterwards at 15-min intervals until PBF returned to the baseline. To assess pulpal anesthesia in both teeth, EPT readings were made before IANB injection, at 2, 4, 6, 8, 10 min post injection, and afterwards at 10-min intervals until the tooth sensitivity returned to baseline. Systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR) were also monitored by the cardiovascular monitoring device (DASH 4000; GE medical systems, Milwaukee, WI, USA) before injection, at 2, 10 min post injection and subsequently at 20-min intervals until the completion of the study.
3
Respiratory Rate Monitoring in ICU Patients
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Patients were monitored (DASH 4000, General Electrics, Fairfield, Connecticut, USA; sample frequency, 100 Hz) for a period of at least 48 hours. Monitoring was performed under routine clinical conditions at the intensive coronary care unit, after performing the acute medical care in the emergency room and catheter lab. The ECG channel of the surveillance monitors was stored on a central server. The respiratory rate was extracted from the ECG recording. Technical details of the methodology of assessment of the respiratory rate from ECG recordings have been described elsewhere.12 To calculate the average NRR, the period from 0:00 to 6:00 from the first night after complete admission was used. In addition, we calculated the NRR from shorter recordings, after just 1 hour (0:00 to 01:00) and 2 hours (0.00 to 02.00).
4
Electrocardiography Monitoring and Analysis
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Electrocardiography (ECG) was performed using a bedside monitor (DASH4000; GE healthcare, Tokyo, Japan), with data transferred to a personal computer. The analog ECG wave output from the monitor was A/D-converted at a sampling rate of 1000 Hz using an A/D-conversion PC card (ADA16-32/2(CB)F, Contec Co., Osaka, Japan). The sequential average instantaneous HR every 10 s was outputted in the CSV format by the HRV analysis software program (MemCalc/Tonam; Suwa Trust, Tokyo, Japan).
5
Continuous Cardiovascular Monitoring Protocol
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A continual ECG Holter monitoring device (MARS 5000, GE Marquette Medical Systems, Milwaukee, United States) was used for measuring heart rate. A Portapress Model 2 (Finapres Medical System B.V. Amsterdam, The Netherlands), was used for non-invasive continuous blood pressure measurement. Additionally, outside the scope of the present study, a patient monitor (Dash 4000, GE Healthcare, Illinois, United States) was used for monitoring of breathing rate and blood oxygen saturation.
6
Radial Artery Pulse Pressure Measurement
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Radial artery pulse pressure sensor (Tuoren, China) which is a mechanical device that does not require a power supply was used. When it is connected with a monitor (Dash 4000, GE company, USA), during the observation, the data are observed in real time (Figure 1(a) ). By using an invasive pressure transducer, we used a three-way valve (Sujia, China) that included a valve connected with the tracheal tube (Tuoren, China), a valve connected with radial artery pulse pressure sensor, and a valve connected with a 10 mL syringe. In order to avoid air leakage during pressure measurement, the air injection valve was closed before each air injection. Radial artery pulse pressure sensor was not linked with the syringe when air was injected into the tracheal cuff so as to avoid inaccurate gas injection (Figure 1(b) ).
7
Etomidate and Remazolam for Gastroenteroscopy
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All patients need regular fasting for 12 hours and drinking for 8 hours before examination. Lateral prone position is taken, oxygen is inhaled by nasal catheter at 3.0 L/min, and ECG and heart rate are monitored by DASH4000 multifunctional monitor (GE). In the single-drug group, 0.9% normal saline is injected, and etomidate (Jiangsu Hengrui Pharmaceutical Co., Ltd., Production lot no. 20171015) is injected 2 min later at a rate of 0.2 mg/(kg/min). On the basis of the single-drug group, anesthesia induction is performed with remazolam (Jiangsu Hengrui Pharmaceutical Co., Ltd., Batch No. 200725) at 12 mg/(kg/h), and anesthesia is maintained at 1.0-2.0 mg/(kg·h). After the patient's consciousness disappeared, gastroenteroscopy (Olympus, Japan, model: CV-170) is performed. According to the patient's physical reaction during the examination, 4–6 mg etomidate is added appropriately. After the gastroenteroscopy reached the ileocecal area, the administration is stopped.
8
Fluid Flow Rates with Needleless Connectors
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We requested sample devices from 8 major manufacturers of needleless connectors available in the United States and received 5 models from 4 manufacturers (Table 1). Flow rates without (control) and with an in-line needleless connector were measured during simulated massive transfusion. Fluids for infusion were room temperature lactated Ringer's solution (Hospira, Lake Forest, IL) or 4 to 10°C RBCs (mixed batches of four units of undiluted, type-matched discarded human banked Adenine-Saline-3 RBCs; Hartford Hospital Transfusion Services). A Level 1 ® H-1200 Fast Flow Fluid Warmer (41°C, D-100 insert; Smiths Medical, St. Paul, MN) delivered fluid under pressure into a graduated cylinder; we determined flow rates by using a stopwatch to determine the amount of time required for a given volume of fluid to be delivered. Delivered volumes were 100 mL for crystalloid trials and 10 to 50 mL (depending on flow rate) for RBC trials. A pressure transducer (ICU Medical, San Clemente, CA) and physiologic monitor (Dash 4000, GE Healthcare, Little Chalfont, Buckinghamshire, UK) measured the output pressure of the Level 1 warmer.
9
Ambulatory Blood Pressure Monitoring
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Before the PWA analysis office brachial diastolic and systolic BP values have been obtained from the portable bedside monitoring automatic BP device (Dash 4000, General Electric Healthcare, Dallas, TX, USA). After PWA analysis ambulatory BP (24hABP) monitoring was done for 24-hour using a Schiller BR-102 plus monitor (Schiller, Switzerland). BP was recorded every 20 minutes during the day and every 30 minutes during the night. The cuff of the BP monitor was applied to the upper portion of the arm, and the patients were instructed to attend to their usual activities and medications.
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