Median whole blood genomic DNA extraction kit (Beijing BioTeke Corp., Beijing, China); HLA-SSO genotyping kit (One Lambda Inc., Canoga Park, CA, USA); ultramicro ultraviolet spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA); PCR amplifier (Applied Biosystems, Foster City, CA, USA); and a flow fluorescence detector (Luminex, Austin, TX, USA) were used in the present study.
Ultra micro ultraviolet spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Ultra-micro ultraviolet spectrophotometer is a laboratory instrument used to measure the absorbance or transmittance of light in the ultraviolet range by small sample volumes. It is designed to analyze the spectral characteristics of materials with high accuracy and precision.
Automatically generated - may contain errors
Lab products found in correlation
Ecl reagent, by Thermo Fisher Scientific (2 mentions)
Mmp 2, by Santa Cruz Biotechnology (2 mentions)
Immun blot pvdf membranes, by Solarbio (2 mentions)
Mmp 9, by Abcam (2 mentions)
β actin, by Abcam (2 mentions)
β actin, by Bio-Rad (2 mentions)
Bcl2 associated x (bax), by Abcam (2 mentions)
Imagej software, by Bio-Rad (2 mentions)
Radioimmunoprecipitation assay buffer, by Solarbio (2 mentions)
Low temperature high speed centrifugal machine, by Thermo Fisher Scientific (1 mentions)
Continuous wavelength multifunctional microplate reader, by Tecan (1 mentions)
4 protocols using ultra micro ultraviolet spectrophotometer
1
Genomic DNA Extraction and HLA Typing
2
Multimodal Neurological Assessments
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Instuments used in the study were: Ultramicro-ultraviolet spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA); continuous wavelength multi-functional microplate reader (TECAN, Salzburg, Austria); WMT-100 Morris water maze video analysis system (Chengdu Taimeng Science and Technology Co., Ltd., Chengdu, China); and low-temperature high-speed centrifugal machine (Thermo Fisher Scientific, Inc.).
3
Protein Expression Analysis of ESCC Cells
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ESCC cells were lysed at 4°C in radio immunoprecipitation assay buffer (Solarbio) mixed with protease and phosphatase inhibitors. The concentration of total protein was assessed using Ultra-micro ultraviolet spectrophotometer (Thermo, USA). Proteinlysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immun-Blot PVDF membranes (Solarbio). and immersed in a blocking solution containing 5% non-fat milk and 0.1% Tween-20 for 1 h.
After blocking, membranes were incubated with speci c primary antibodies against Bcl-2 (Beyotime biotechonology), Bax (Abcam), MMP-9 (Abcam), MMP-2( Santa Cruz) and β-actin (Abcam) at 4°C overnight, and then with secondary antibodies for 2 h at room temperature. After washing, The blots were developed with ECL reagents (Thermo), quanti ed by densitometry, then analyzed using the Image J software (BIO-RAD Company, USA), and normalized to the corresponding β-actin bands.
After blocking, membranes were incubated with speci c primary antibodies against Bcl-2 (Beyotime biotechonology), Bax (Abcam), MMP-9 (Abcam), MMP-2( Santa Cruz) and β-actin (Abcam) at 4°C overnight, and then with secondary antibodies for 2 h at room temperature. After washing, The blots were developed with ECL reagents (Thermo), quanti ed by densitometry, then analyzed using the Image J software (BIO-RAD Company, USA), and normalized to the corresponding β-actin bands.
4
Protein Expression Analysis of ESCC Cells
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ESCC cells were lysed at 4°C in radio immunoprecipitation assay buffer (Solarbio) mixed with protease and phosphatase inhibitors. The concentration of total protein was assessed using Ultra-micro ultraviolet spectrophotometer (Thermo, USA). Proteinlysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immun-Blot PVDF membranes (Solarbio). and immersed in a blocking solution containing 5% non-fat milk and 0.1% Tween-20 for 1 h.
After blocking, membranes were incubated with speci c primary antibodies against Bcl-2 (Beyotime biotechonology), Bax (Abcam), MMP-9 (Abcam), MMP-2( Santa Cruz) and β-actin (Abcam) at 4°C overnight, and then with secondary antibodies for 2 h at room temperature. After washing, The blots were developed with ECL reagents (Thermo), quanti ed by densitometry, then analyzed using the Image J software (BIO-RAD Company, USA), and normalized to the corresponding β-actin bands.
After blocking, membranes were incubated with speci c primary antibodies against Bcl-2 (Beyotime biotechonology), Bax (Abcam), MMP-9 (Abcam), MMP-2( Santa Cruz) and β-actin (Abcam) at 4°C overnight, and then with secondary antibodies for 2 h at room temperature. After washing, The blots were developed with ECL reagents (Thermo), quanti ed by densitometry, then analyzed using the Image J software (BIO-RAD Company, USA), and normalized to the corresponding β-actin bands.
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