Goldview

All DNA oligonucleotides (Supporting Information, Table S1) were synthesized and purified by Sangon (Shanghai, China). The DNA oligonucleotides were dissolved in 1 × Tank buffer (20 mM Tris,125 mM NaCl, 20 mM KCl, pH 7.5) and diluted in appropriate buffer prior to use or stored at −20 °C. DNA Marker, GoldView, SYBR Premix Ex TaqTM II and PrimescriptTM RT reagent Kit with gDNA Eraser were purchased from TaKaRa (Dalian, China). Dulbecco’s Modified Eagle Medium (DMEM) and Fetal Bovine Serum (FBS) were obtained from Gibco (Shanghai, China). CCK-8 assay was obtained from Dojindo (Shanghai, China). Phospho-HER2/ErbB2 Antibody Sampler Kit and β-Actin polyclonal antibody were obtained from Cell Signaling Technology (Shanghai, China). HRP anti-mouse and rabbit IgG were from Beyotime (Jiangsu, China).New Super ECL Assay was from KeyGen (Nanjing, China). RIPA (50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton x-100, 1% sodium deoxycholate, 0.1% SDS, sodium orthovanadate, sodium fluoride, EDTA, and leupeptin), phenylmethanesulfonyl fluoride (PMSF) and Bradford protein dye reagent were used in western blot. SK-BR-3and MDA-MB-231 (the human breast cancer cell lines) cells were obtained from the American Type Culture Collection (ATCC) (Rockville, MD, USA). All other reagents were of analytical grade, and Millipore-Q water (≥18 МΩ) was used in all experiments.

Xanthomonas oryzae pv. Oryzae (CCTCC AB 91123) was obtained from China-Center for Type Culture Collection, Wuhan University (Wuhan, China), and cultured as previously described (Byul et al. 2011 (link)). Both E. coli DH5α used for gene cloning and E. coli BL21 (DE3) serving as an expression host were purchased from TaKaRa Biotechnology Co. Ltd (Dalian, China). E. coli strains were routinely grown in LB broth or on LB agar plates with 100 µg/ml of ampicillin at 37 °C. The pMD19-T vector and pET-22b (+) vector were purchased from Invitrogen Corp. (Shanghai, China), and used for cloning and expression studies, respectively.
DNA Marker, Protein Marker, GoldView, T4 DNA ligase, PrimeSTAR HS DNA Polymerase, QuickCutTM Nde I and QuickCutTM Xho I restriction enzymes were purchased from TaKaRa Biotechnology Co., Ltd (Dalian, China). A bacterial genome extraction kit, a DNA fragment purification kit, an agarose gel DNA purification kit and a MiniBEST Plasmid Purification Kit were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China). All aqueous solutions and buffers were prepared with water purified with an in-house Milli-Q Plus System (Millipore, Inc., Billerica, MA, USA). All the other chemicals were of analytical grade.