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6 protocols using toluene

1

Preparation of Stimulus-Responsive Polymer Particles

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All reagents, methacrylic acid (MAA), 2-vinylpyridine (2-VP), ethylene glycol dimethacrylate (EDGMA), azobisisobutyronitrile (AIBN), polyvinyl alcohol (PVA), lauryl sulfate (SDS), formic acid, diclofenac (DCF) sodium, and naproxen (NPX) were purchased from Sigma-Aldrich (Darmstadt, Germany). The organic solvents, methanol, and toluene were obtained from Tedia Company Inc. (Fairfield, Ohio, USA). Hydrochloric acid (HCl) and potassium hydroxide (KOH) were supplied by Merck (State of Mexico).
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2

Polyelectrolyte Multilayer Coatings

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Poly(isobutylene-alt-maleic anhydride) (PIAMA, Mw: 6 kDa), poly(allylamine hydrochloride) (PAH, Mw: 58 kDa), l-histidine methyl ester dihydrochloride, 3-aminopropyltrimethoxysilane (APTMS), N,N-diisopropylethylamine (DIPEA), copper(ii) nitrate trihydrate (Cu(NO3)2 × 3H2O), sodium chloride, sea salt and sodium hydroxide (all from Sigma Aldrich), N,N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), toluene, methanol, acetone and isopropanol (all from Tedia) were used directly as received without further purification. Dialysis membrane tubing (MWCO: 3.5 kD) was received from Fisher Scientific. Silicon wafers (Latech Scientific Supply Pte. Ltd) were 0.6 mm thick, with one side polished and with a natural silicon dioxide layer. QSX 303 Silicon dioxide 50 nm quartz crystal microbalance (QCM) chips were obtained from Analytical Technologies Pte Ltd. Deionized (DI) water with 18 MΩ cm–1 resistivity was obtained from a Millipore Nanopure system.
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3

Synthesis and Characterization of Poloxamer-Based Biomaterials

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O,O’-Bis(2-aminopropyl) polypropylene glycol-block-polyethylene glycol-block-polypropylene glycol (Poloxamer, PLX, PPG–PEG–PPG, Mn = 900), l-alanine, hydrogen bromide (HBr/acetic acid), and a LIVE/DEAD staining kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tacrolimus (FK506) was obtained from LC Labs (Woburn, MA, USA). Trifluoroacetic acid-d (TFA-d) and trifluoroacetic acid (TFA) were purchased from Alfa Aesar (Wall Hill, MA, USA). l-lysine-(Z) was commercially obtained from ACROS (Morris Plains, NJ, USA). Diethyl ether, hexane, and ethanol (95%) were purchased from Echo Chemicals (Toufen, Miaoli, Taiwan). Dimethyl sulfoxide (DMSO) was obtained from J.T. Baker. Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), and antimycotics–antibiotics were purchased from Gibco (Grand Island, NY, USA). Toluene and tetrahydrofuran (THF) were obtained from TEDIA (Fairfield, OH, USA), and chloroform, dichloromethane (DCM), and N,N-dimethylformaide (DMF) were purchased from AVANTOR (Center Valley, PA, USA). All solvents were dried over CaH2 before used.
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4

Luminescent Polymer Sensor Fabrication

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The glass substrate (0.7 mm) was purchased from Corning (New York, NY, USA); platinum(II) meso-tetrakis (pentafluorophenyl) porphyrin (PtTFPP) was purchased from Frontier Scientific, (Logan, UT, USA); cellulose acetate (CA) powder was purchased from Showa Chemicals (Tokyo, Japan) and ethyl cellulose (EC) was purchased from Tokyo Chemical Industry Co., LTD (TCI) (Chuo City, Japan). Other reagents such as EtOH (99.5%) and SiO2 (4 micron, 99.9%) were purchased from ECHO Chemical co Ltd. (Miaoli, Taiwan). Eosin Y (99%) and acetic acid (99%) were purchased from Sigma Aldrich (St. Louis, MO, USA). Tetrahydrofuran (THF, 99.9%) and toluene (99.8%) were purchased from TEDIA (Fairfield, CT, USA). The received chemicals were used without any further purification.
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5

Pesticide Quantification Protocol

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The pesticides malathion 97.8 %, chlorpyrifos ethyl 99.1%, chlorothalonil 99.5%, and atrazine as the internal standard 99.2% were purchased from AccuStandar Inc. (New Haven, CT, USA). All solvents—toluene, acetonitrile, methanol, acetic acid, formic acid, and water—were obtained from Tedia (Carson City, CA, USA).
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6

Analytical Protocols for Environmental Samples

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All chemicals used in the digestion process are extra pure: hydrochloric acid (36.5–38.0%); hydrofluoric acid and nitric acid (69.0–71.0%) (Merck Sigma-Aldrich, USA); optima grade hydrogen peroxide (H2O2) assay (30–32%) (Fisher Scientific, UK); DIW-ASTM Type I (deionized water, ultrapure water, Type I water is defined by the American Society for Testing and Materials (ASTM)).
The chemical products used for extraction and cleaning were purchased as follows: Florisil® 0.15–0.25 mm (60–100 mesh) from Merck, Germany; silica gel 60, 0.063–0.200 mm (70–230 mesh) from Merck, Germany; and extra pure anhydrous sodium sulfate (Fluka, Switzerland). Solvents used are as follows: dichloromethane of 97% purity, n-hexane of 97% purity, and toluene of 98% purity were purchased from Tedia, USA; petroleum ether (40–60°C) of 95% purity was purchased from Carlo Erba, Italy; ethanol absolute of 99% purity, n-nonane of 98.7% purity, and isooctane of 98.7% purity were purchased from Riedel de-Haën, Hannover, Germany; acetone of 99% purity was purchased from GCC, UK.
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